The aim of this study is to find a suitable alternative to fetal bovine serum for amplification of mesenchymal stem cells products and study the differentiation potential of the amplication products.Adherent screening adult bone marrow mesenchymal stem cells were isolated and cultured,and different passages of the expanded cells were used to differentiate into osteoblasts and fat cells.Osteoblasts were identified by Vonkossa,alkaline phosphatase staining of osteoblasts,and fat cells by oil red staining of adipogenic.In the configuration with 2% adult serum complete medium,different passages of adult bone marrow mesenchymal stem cells still had a bone,adipogenic differentiation potential.The adult bone marrow mesenchymal stem cells expanded in this medium can be incuced into osteoblasts,fat cells.The proposed method may be ideal for expansion of adult bone marrow mesenchymal stem cells in vitro.The amplication products may be useful to bone tissue engineering.

The aim of this study was to explore the changes in cellular senescence related indexes of bone marrow mesenchymal stem cells (BMMSCs) after total body irradiation (TBI). At different time points after 4 Gy irradiation, BMMSCs were isolated from male C57BL/6 mice and cultured. Morphology, senescence-associated beta-galactosidase (SA-beta-gal) staining and cell cycle analysis were used to evaluate the changes in BMMSCs at cellular level while real-time RT-PCR was used to detect the alterations in senescence related gene expression including p16INK4a, p21Cip1/Waf1, p53 and TGF-beta1. The results showed that within 4 weeks after exposure to 4 Gy TBI, the morphology of BMMSCs and the expression level of SA-beta-gal were not significantly changed, the cellular senescence-related cell cycle arrest was not occurred and the senescence related gene expression level was not increased. It is concluded that at the early stage after 4 Gy TBI, the related molecular level of cellular senescence in BMMSCs is not changed.