The hydrolytic conditions of konjak gum for producing glucomannan oligosaccharides were studied with high activity β-mannanase from Aspergillus niger E-56.Based on single factor test,the optimum conditions were obtained by orthogonal test.The optimum hydrolytic conditions were as follows: konjak gum solution 240 g/L(with deionized water),hydrolytic temperature 50 ℃,β-mannanase dosage 120 U/g konjak gum,and hydrolytic time 8 h.With these conditions,the range of average polymerization degree(DP) was 1.8~1.9 in hydrolysates.

Based on the tests of solid-state fermentation by Aspergillus niger LW-1-9 strain with shake flask and koji tray,the industrial production of β-mannanase was carried out in 30 m3 solid-state fermentor(Φ 4.5×2.0 m).The amount of base material(wheat bran 700~800 kg,soybean or rapeseed cake flour 200~300 kg) was 1000 kg,the ratio of based material to waster was 1∶1.8~2.0,natural pH,complemented with konjac powder 3~5%,corn steep liquor 3~5%,(NH4)2SO4 1~1.5%,CaCl2 0.05~1%,MgSO4 0.05~0.1%,KH2PO4 0.3~0.5%(related to based materials).The β-mannanase activity reached 26 725~29 218 IU/g dry koji at 32~36 ℃ for 72~84 h by adding water and turning koji at 20~24 h and 36~40 h.

在大田条件下研究了不同基础群体和结构对中药元胡产量的影响.结果表明,元胡的产量随着基础群体的增大而增加,且增加的主要是二级品产量(直径≤10 mm);基础群体大小相同,群体结构不同时,产量有明显的差异,并以每穴1棵种茎种植方式的元胡总产量和优质元胡产量最高,但优质元胡所占的百分比则以每穴3棵时最大,为84.17%.

探讨了不同浓度几丁聚糖对青花菜花球的酚类物质和品质的影响,结果表明,浓度为60 mg/L几丁聚糖对花球的生长影响不大,浓度为50 mg/L几丁聚糖使花球的酚类物质及品质有所提高,浓度为40 mg/L时则能显著提高花球酚类物质含量及品质,其中叶绿素、类黄酮、绿原酸、可溶性总糖、可溶性蛋白分别较对照提高64.4%、282.5%、35.6%、16.2%、22.7%,但蔗糖含量却有所降低;并初步探讨了酚类物质与跟其形成有关的一些酶(PAL、PPO)的相互关系.