OBJECTIVE:To study the effects of Jinmaitong capsule on oxidative stress and cell apoptosis of dorsal root ganglion (DRG) in rats with diabetic peripheral neuropathy.METHODS:Sixty male SD rats were randomly divided into normal group and model groups. The diabetic rat models were established using Streptozotocin (STZ) method (60 mg/kg of intraperitoneal injection), and then randomly divided Jinmaitong low, middle, and high-dose groups and vitamin C group. All the experimental rats were sacrificed at 16-week and then the DRG was isolated. The morphological changes of DRG were observed using the Nissl's staining, and the NADPH oxidase subunit p22-phox, Cyt C, Bcl-2, and Caspase-3 of DRG in rats were detected by immunohistochemistry and quantitative reverse transcription PCR (qRT-PCR). Cell apoptosis was detected by TUNEL.RESULTS:Compared with the model group, the expressions of NADPH oxidase subunit p22-phox protein, Cyt expression of C protein, Caspase-3 protein, and mRNA cell apoptosis rate in each treatment group significantly decreased whereas the expressions of Bcl-2 mRNA and protein significantly increased (P<0.05 or P<0.01). The Jinmaitong high-dose group had the best effect and was significantly different from that of the vitamin C group (P<0.01).CONCLUSIONS:Jinmaitong capsule can prevent the nerve injury in rats with diabetic peripheral neuropathy by inhibiting oxidative stress and decreasing the apoptosis. The high-dose Jinmaitong capsule has the best effect and is superior to vitamin C.

Objective:To establish a headspace GC fingerprint chromatogram for analysis volatile compounds of Fufang Nanxing Zhitong Gao,and evaluated its quality.Methods:The sample was conducted by GC with HP-5 column(30 m×0.25 mm,0.25 μm).The carrier gas was N2 at the flow rate of 1.0 mL · min-1.Split ratio was 5:1.The detection was FID.Ten batches of Fufang Nanxing Zhitong Gao was calculated to their similarity.Results:Six main peaks were clearly separated in 20 min.The similarity of ten batches was over 0.95.Conclusion:The headspace GC fingerprint chromatogram is successfully used on analysis and quality control of Fufang Nanxing Zhitong Gao.

文章通过对风吹雪雪害地区不同路基高度的风雪流场的观测与分析,初步掌握了公路路基高度与风雪流场的关系,确定了风吹雪雪害地区不易积雪的合理路基高度,为公路风吹雪雪害防治技术研究提供有力的科学依据。