Objective To observe the expression levels of p27 and PCNA protein in patients with chronic gastric diseases and in patients with gastric cancer,and to explore the correlation between the expressions of p27,PCNA protein and pathogenesis of gastric cancer.Methods A total of 155 biopsy specimens of gastric mucous membrane by gastroscopy were collected including 32 cases of chronic non-atrophic gastritis,25 cases of chronic atrophic gastritis, 30 cases of intestinal metaplasia,32 cases of atypical hyperplasia and 36 cases of gastric carcinoma. The expression levels of p27 and PCNA protein in gastric mucosa were detected by immunohistochemistry (SP) and statistically analyzed. Results The colouring of P27 protein in gastric cancer tissue superficial parts was relatively strong,however,which in deep part was relatively weak. The expression levels of p27 were gradually decreased in the order of chronic non-atrophic gastritis, chronic atrophic gastritis, intestinal memplasia,atypical hyperplasia and gastric carcinoma,however, the expression levels of PCNA were gradually increased with the order,there were significant differences between two groups each other(P<0.05).Moreover the expression levels of P27 were negatively correlated with chronic non-atrophic gastritis, chronic atrophic gastritis,intestinal memplasia, atypical hyperplasia and gastric carcinoma,(r= -0.976,P<0.01),however,the expression levels of PCNA were positively correlated with the disease types mentioned above (r=0.979,P<0.05).In addition the the expression levels of P27 were negatively correlated with those of PCNA among the 5 groups(r= -0.920,P<0.01).Moreover the expressio nlevels of P27 and PCNA protein were not correlated with patient's age and sex,location and size of tumor (P>0.05).Conclusion The expression levels of P27 and PCNA protein in gastric mucosa are correlated with the pathogenesis and development of chronic gastric diseases,gastric precancerous lesion and gastric cancer,which play an important role during the development process of gastric cancer.

Objective To study the expression of p27 protein and apoptosis index in gastric mucosa of patients with chronic gastric diseases and patients with gastric cancer, and to explore the relationship between the expression of p27 protein in gastric cancer and the evolution of AI. Methods A total of 155 mucosal biopsy specimens including 32 cases of chronic nonatrophic gastritis, 25 cases of chronic atrophic gastritis, 30 cases of intestinal metaplasia, 32 cases of atypical hyperplasia and 36 cases of gastric cancer from outpatients were enrolled in the study. The expression levels of p27 protein and apoptosis index ( AI) were detected by immunohistochemical SP method. Results Yellow or brown granules were located in the nucleus in gastric cancer tissues, with relatively weak staining in superficial layer and relatively strong staining in deeper layer. The expression levels of P27 protein were gradually decreased with the progression of disease. There were significant differences between the groups each other (P<0. 05). The positive staining of apoptotic cells were located in the nucleus, with condensed cytoplasm against the nuclear membrane or stained evenly, without inflammatory reaction around. With the progression of disease ( chronic nonatrophic gastritis, chronic atrophic gastritis, intestinal memplasia, atypical hyperplasia, gastric cancer) , AI was increased gradually in the first three stages, then decreased gradually in the last stages, and there were no significant differences between the first three groups each other (P > 0. 05),however, there were significant differences between the last three groups each other (P<0. 05). In addition, the expression of p27 protein in gastric mucosa of the patients was negatively related to the type of disease (r=0. 976,P<0. 01). However there was no correlation between the expression levels of AI and the type of disease (r=0. 060,P>0. 05),moreover, the expressions levels of p27 protein and AI were not correlated with patient's age, gender, the location and size of the tumor (P>0. 05). Conclusion The expression levels of P27 protein and cell apoptosis are related with the pathogenesis and development of chronic gastric diseases, gastric precancerous lesions and gastric cancer, which play an important role in the evolution of gastric cancer.

Objective To study the expression of p27 proteins and cyclin B1 in gastric mucosa in patients with chronic gastritis and gastric carcinoma,and their correlations with pathogenesis of gastric carcinoma. Methods Immunohistochemical SP method was used to investigate the expression levels of p27 and cyclin B1 in chronic non-atrophic gastritis,chronic atrophic gastri-tis,intestinal metaplasia,atypical hyperplasia and gastric carcinoma. Results From chronic non-atrophic gastritis to chronic a-trophic gastritis,intestinal metaplasia,atypical hyperplasia and the final gastric carcinoma,the expression levels of p27 proteins decreased while cyclin B1 increased,showed a negative correlation. Conclusion The expression levels of p27 proteins and cyclin B1 are related to chronic gastritis,gastric lesion and gastric carcinoma,both take significant roles in the development of gastric carcinoma.

目的:观察自拟金海排石方治疗泌尿系结石的临床疗效.方法:将120例泌尿系结石患者按就诊顺序随机分为两组,治疗组75例采用自拟金海排石方治疗,对照组采用排石冲剂口服治疗.排石冲剂(南京同仁堂药业有限公司,规格为6%/包)20g,3次/d,连服30d.均一个月为1个疗程,自用药之日起记录患者结石大小变化情况及血尿、尿蛋白变化情况.治疗2个疗程后比较临床疗效及治愈一年后复发情况,用SPSS 16.0软件对统计数据进行分析.结果:2个疗程后治疗组疗效优于对照组(P＜0.05),一年后治疗组复发率低于对照组(P＜0.05).结论:自拟金海排石方疗效肯定、辨证加减灵活、无创伤性、不良反应少,并且能够有效降低复发率,为治疗泌尿系结石的新的治疗途径,在临床上值得广泛应用.

Objective To investigate the influence of Bailing capsule combined with Irbesartan on the patients with idiopathic membranous nephritic(IMN) syndrome and immunologic function.Methods 76 patients of IMN were randomly divided into two groups,the control group was treated with irbesartan,and the treatment group was treated with irbesartan combined with Bailing capsule.After 3 months of treatment 24 h urinary protein excretion,serum albumin(Alb) and Cr level were observed before and after treatment and T lymphocyte subsets was analyzed.Results After 3 months,24 hours urine protein and Cr level of two groups were all dropping,albumin were all rising,but the change of the treatment group was more obvious.There was statistically significant difference between two groups on T lymphocyte subsets after treatment(P < 0.05).The level of CD3 and CD4 in treatment group was raised compared with that of control group,CD8 was dropped,and the decrease of CD4 / CD8 was obvious.Conclusion The combination therapy of Bailing capsule and irbesartan can obviously decrease 24 hours urine protein and rise albumin,enhance immunity.

目的 观察阿托伐他汀在治疗冠状动脉粥样硬化性心脏病(冠心病)过程中的白细胞介素6(interlerkin-6,IL-6)及脂联素(adiponectin,APN)水平动态变化过程.方法 选择冠心病患者160例,随机分为对照组和治疗组各80例,2组均按冠心病伴高脂血症常规内科治疗,疗程8周.治疗组加用阿托伐他汀20mg口服,1次/d.分别于治疗前及治疗4周、8周时采集患者肘静脉血,ELISA法检测后比较2组IL-6及APN水平动态变化.结果 与治疗前比较,2组治疗后IL-6水平均减低,APN水平均升高,且治疗组尤其明显,差异有统计学意义(P＜0.05).结论 应用阿托伐他汀治疗冠心病能显著降低患者IL-6水平、升高APN水平.

目的探讨阿托伐他汀在治疗冠心病中的抗炎作用机制。方法选择2010至2011年门诊及住院冠心病患者120例,随机分为观察组和研究组,每组60例,2组均按CHD伴高脂血症常规内科治疗,疗程均为8周。研究组加用阿托伐他汀20mg,1次/d,口服。比较2组血清C-反应蛋白、TNF-α等水平变化情况,应用Westernblot方法检测NF-κBp65。结果治疗8周后,研究组血清C-反应蛋白、TNF-α等均较治疗前显著下降(P<0.05),而对照组治疗前后上述指标差异无统计学意义(P>0.05)。结论阿托伐他汀治疗后患者血浆CRP、TNF-α水平明显下降,其可能的作用机制是抑制NF-κB通路活化。

Objective To observe the effect and course of acupuncture treatment of very early in treating facial neuritis.Methods 170 patients with facial neuritis in acute stage were randomly divided into two groups.86 cases in treatment group were given ipsilateral superficial acupuncture in acute stage,conventional differentiation of acupuncture in quiescent stage and recovery phase.84 patients in control group were just given conventional differentiation of acupuncture in quiescent stage and recovery phase.Facial nerve function was evaluated in before and different times after treatment.The recovery rate and time of therapy to recovery within six weeks were compared.Results The scores of facial nerve function in treatment group were higher than those in control group two weeks after treatment(P<0.05,P<0.01).There was obvious difference between two groups on grading facial nerve function of House-Brackmann at 3,6 weeks after treatment(P<0.05,P<0.01).The total effective rate in treatment group was higher than that in control group(P<0.05).The course of treatment in treatment group was significantly shorter than that of the control group(P<0.05).Conclusion Acupuncture treatment of very early in treating facial neuritis can improve facial nerve function,enhance therapeutic effect and shorten course of treatment.