Objective To investigate the expression and clinical significance of Mina53 protein in colon adenoma and colon carcinoma.Methods Ninety colon adenoma samples of different pathological types were collected,including 30 cases of tubular adenoma,30 cases of tubulovillous adenoma,30 cases of villous adenoma and 60 cases of colon carcinoma.There were 60 cases of normal tissue specimens.The protein and mRNA levels of Mina53 were detected by immunohistochemistry and Real-time quantitative PCR respectively.Results The positive expression of Mina53 in normal mucosa group,colon adenoma group and colon carcinoma group was 0.0％,36.7％ and 68.3％,respectively (P =0.000).The positive expression of Mina53 in tubular adenoma group,tubulovillous adenoma group and villous adenoma group was 26.7％,33.3％ and 50.0％,respectively (P =0.001).Fluorescence quantitative PCR showed that the expression of Mina53 mRNA in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma was 9.016 8 ± 0.720 3,31.788 5 ± 3.681 6,43.121 4 ± 4.9378,45.9634±4.3137,49.9005±6.131 1 respectively (P=0.016).Conclusion The expression of Mina53 protein is higher in colon cancer than the colon adenoma and normal colon mucosa,suggesting that Mina53 may have a functional role in colon carcinogenesis and may be used as a marker for colon carcinoma.

Objective To explore the expression of protease activated receptor 3 (PAR3) and protease activated receptor 4 (PAR4) in the progression from colorectal polyps to cancer.Methods In this study,five groups of specimens were researched:primary colorectal cancer group (n =30),matched normal colorectal tissues group (taken 5 cm away from the tumour) (n =30),tubular adenoma group (n =30),villous adenoma group (n =20),and adenoma mixed group (n =30).The protein and mRNA levels of PAR3 and PAR4 were detected by immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) respectively.Results The positive expression rate of PAR3 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues was 73.3％,50.0％and 6.7％ respectively,with significant differences among them (P =0.009).The positive expression rate of PAR4 protein in these groups was 6.7％,35.0％ and 70.0％ respectively.FQ-PCR showed that the expression of PAR3 mRNA in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma was 0.787 ± 0.040,0.453 ± 0.023,0.410 ± 0.050,0.368 ±0.032 and 0.259 ± 0.017,P =0.0l 1;and that of PAR4 mRNA in these groups was 0.370 ± 0.301,10.384 ± 1.474,20.892 ± 4.485,36.311 ± 7.953,52.083 ± 12.550 (F =43.342,P =0.009,respectively).Conclusion These results suggested PAR3 and PAR4 may play a role in the development of colorectal cancer.

OBJECTIVE To investigate the effects of transforming growth factor beta (TGF ) on c-fos gene expression in hepatic stellate cells. METHODS Hepatic stellate cells (HSC-T6) were cultured in the medium containing different concentrations of TGF (0.2, 1, and 5 ng/ml), and cells were collected at different time points of incubation (8, 24, 48, and 72 h). The total RNA of the HSCs was isolated and c-fos gene expression level were measured by reverse transcription polymerase chain reaction. RESULTS c-fos gene expression levels of HSCs cultured in the presence of low (0.2 ng/ml), moderate (1 ng/ml) and high (5 ng/ml) concentrations of TGF for 8, 24, 48 and 72 h were significantly greater than those of control group. The c-fos gene expression levels of HSCs increased gradually with the increment of TGF concentration, and significant differences in c-fos gene expression were found between the 3TGF groups. CONCLUSION TGF strongly up-regulates c-fos gene expression in hepatic stellate cells.