Objective To detect the expression of Claudin-7 protein in the normal mucosa, colorectal adenoma with different pathological types and colorectal carcinoma with different stages and study its implication in different stages of colon carcinogenesis.Methods Eighty cases of colorectal adenoma samples with different pathological types removed under endoscopy were collected, which included 30 cases of tubular adenoma, 30 cases of tubular villous adenoma, and 20 cases of villous adenoma.The samples also included 60 cases of colorectal carcinoma specimens and 40 cases of paracancerous normal tissue specimens resected surgically.Immunohistochemistry was applied to detect the expression of Claudin-7 protein in the tissues.Results The positive rate of Claudin-7 expression in normal mucosa was 100%, however, that in colorectal adenoma and colorectal carcinoma was significantly decreased, and that was the lowest in colorectal carcinoma (46.7%).The difference had statistical signifycance (P=0.000).The expression of Claudin-7 was associated with the Dukes staging, the degree of differentiation and the lymph node metastasis.The positive rate of Claudin-7 expression in tubular adenoma group, mixed pattern of adenoma group and villous adenoma group was 83.3%, 63.3% and 50.0%, respectively, with significant differences among them (P=0.040).Conclusion The expression of Claudin-7 inhibited the occurrence and development of colorectal carcinoma, and appeared abnormal in early colorectal carcinoma.

Objective Detecting the expression of Claudin-1 protein in the normal mucosa,colorectal adenoma with different pathological types and colorectal carcinoma with different stages and probing its meaning in different stages of colon carcinogenesis.Methods 80 colorectal adenoma samples of different pathological types resected endoscopically were collected,including 30 cases of tubular adenoma,30 cases of tubular villous adenoma,and 20 cases of villous adenoma.Furthermore,the samples also included 60 cases of colorectal carcinoma specimens and 40 cases of paracancerous normal tissue specimens resected by general surgery.Immunohistochemistry was applied to detect the expression of Claudin-1 protein in the tissue samples.Results The positive rate of Claudin-1 expression in normal mucosa group,colorectal adenoma group and colorectal carcinoma group was 20.0％,46.2％ and 81.7％ respectively,with the statistically significant difference (P =0.000).A significant rise in its expression from colorectal adenoma,to colorectal carci noma gradually increased.And the expression of Claudin-1 was associated with the Dukes staging and the lymph node metastasis.The positive rate of Claudin-1 expression in tubular adenoma group,mixed pattern of adenoma group and villous adenoma group was 33.3％,43.3％ and 70.0％ respectively,with significant differences among them (P =0.036).Conclusion The expression of Claudin-1 promotes the occurrence and development of colorectal carcinoma.The changes of Claudin-1 probably imply the occurrence of early colorectal carcinoma,and/or the malignant transformation risk of colorectal adenoma.

Objective To investigate the expression and clinical significance of Mina53 protein in colon adenoma and colon carcinoma.Methods Ninety colon adenoma samples of different pathological types were collected,including 30 cases of tubular adenoma,30 cases of tubulovillous adenoma,30 cases of villous adenoma and 60 cases of colon carcinoma.There were 60 cases of normal tissue specimens.The protein and mRNA levels of Mina53 were detected by immunohistochemistry and Real-time quantitative PCR respectively.Results The positive expression of Mina53 in normal mucosa group,colon adenoma group and colon carcinoma group was 0.0％,36.7％ and 68.3％,respectively (P =0.000).The positive expression of Mina53 in tubular adenoma group,tubulovillous adenoma group and villous adenoma group was 26.7％,33.3％ and 50.0％,respectively (P =0.001).Fluorescence quantitative PCR showed that the expression of Mina53 mRNA in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma was 9.016 8 ± 0.720 3,31.788 5 ± 3.681 6,43.121 4 ± 4.9378,45.9634±4.3137,49.9005±6.131 1 respectively (P=0.016).Conclusion The expression of Mina53 protein is higher in colon cancer than the colon adenoma and normal colon mucosa,suggesting that Mina53 may have a functional role in colon carcinogenesis and may be used as a marker for colon carcinoma.

Objective To investigate the expression and significance of human cervical canceroncogene (HCCR-1) protein in colon carcinoma,colon adenoma and normal colon mucosa.Methods Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) were used to detect the location and expression of HCCR-1 in the 80 colon adenoma samples,30 colon carcinoma,20 normal colon mucosa.Results The positive expression rate of HCCR-1 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues were 10.00％,62.50％,100.00％,respectively,with significant differences among them(P =0.001).The positive expression rate of HCCR-1 protein in tubular adenoma,mixed pattern of adenoma and villous adenoma were 46.70％,66.70％,80.00％,respectively,with significant differences among them(P =0.015).FQ-PCR showed that the HCCR-1 mRNA expression in normal colorectal mucosa,tubular adenoma,mixed pattem of adenoma,villous adenoma and colorectal carcinoma tissues were 0.609 2 ± 0.326 1,4.034 6 ± 1.188 4,12.353 4 ± 2.828 0,22.563 7 ± 78.952 0,43.202 9 ± 7.675 4.Conclusion HCCR-1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma.

Objective To investigate the expression and significance of vascular endothelial growth factor (VEGF) in gastric adenocarcinoma, normal gastric mucosa and chronic atrophic gastritis with low grade intraepithelial neoplasia.Methods Immunohistochemical streptavid-in-peroxidase (SP) method was used to detect the expression levels of VEGF in 65 cases of gastric cancer tissues, 50 cases of chronic atrophic gastritis with low grade intraepithelial neoplasia, and 35 cases of normal gastric mucosa (GM).The clinical data were reviewed in 65 patients with gastric adenocarcinoma.Results VEGF overexpression was seen in 2 cases of GM (2/35, 5.7%), 18 cases of chronic atrophic gastritis with low grade intraepithelial neoplasia (18/50, 36.0%), and 36 cases of gastric adenocarcinoma (36/65, 55.4%).Overexpression rate of VEGF in chronic atrophic gastritis with low grade intraepithelial neoplasia and gastric adenocarcinoma was significantly higher than that in the normal GM (P=0.001).Conclusion VEGF protein may play a certain role in the progression of gastric adenocarcinoma.It may also have significantly positive association with the oncegenesis and prognosis of the gastric cancer.

Objective To detect the expression levels of Pokemon in colorectal carcinoma, colorectal adenomas and normal colorectal mucosa, and analyze the relationships among different histological types of colorectal adenomas and colorectal carcinoma.Methods Sixty cases of colorectal carcinoma tissues, 80 cases of colorectal adenomas, and 40 cases of normal colorectal mucosa tissues were collected.Immunohistochemistry and fluorescence quantitative polymerase chain reaction (PCR) were used to detect the expression of Pokemon in the tissues.Results The immunohistochemistry revealed that the positive expression rate of Pokemon protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 25.0%, 51.2% and 80.0%, respectively, with significant differences among them (P=0.000).The positive expression rate of Pokemon in tubular adenoma, mixed pattern of adenoma and villous adenoma was 33.3%, 53.3% and 75.0%, respectively, also with significant differences among them (P=0.015).There was a significant correlation between the raised expression of Pokemon and unfavorable variables, including nodal metastasis (P=0.040), Dukes stage (P=0.014), and differentiation degree (P=0.004).There was no obvious difference in age (P=0.947) and sex (P=0.795).Fluorescence quantitative PCR showed characterized amplification about Pokemon.The expression levels of Pokemon mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma were 0.981±0.189, 2.024±0.226, 3.357±0.262, 5.006±0.281, and 6.450±0.216 respectively,F=432.884,P=0.012.Conclusion Pokemon participates in the occurrence and development of colorectal adenoma and colorectal carcinoma.

Objective To explore the expression of minichromosome maintenance protein 2 (MCM2) in normal colorectal mucosa, colorectal adenomas and colorectal carcinoma, and to analyze the relationships among different histological types of colorectal adenomas and colorectal carcinoma.Methods Immunohistochemistry was used to exzamine the location and expression of MCM2 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues.Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect MCM2 mRNA and connexin-32 mRNA expression.Results (1) Immunohistochemistry: The positive expression rate of MCM2 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 16.7%, 55.0% and 80.0% respectively, with significant differences among them (P=0.031).The positive expression rate of MCM2 in tubular adenoma, mixed pattern of adenoma and villous adenoma was 40.0%, 65.0% and 75.0% respectively, also with significant differences among them (P=0.022).(2) FQ-PCR: The MCM2 mRNA expression in the normal colorectal mucosa, tubular adenoma, the mixed pattern of adenoma, villous adenoma, and colorectal carcinoma was 1.187±0.923, 4.126±1.339, 9.577±0.838, 22.150±4.077 and 48.020±4.811, respectively.The expression of MCM2 gradually increased in colorectal carcinoma, villous adenoma, the mixed pattern of adenoma, tubular adenoma, and normal colorectal mucosa (F=55.512,P=0.029).SNK multiple comparisons displayed that the differences about any two groups of the total mean showed statistical significance.Conclusion MCM2 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma.Detecting the expression of MCM2 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma.They may be the molecular biological indicators for predicting canceration of colorectal adenoma.

Objective To explore the expression of protease activated receptor 3 (PAR3) and protease activated receptor 4 (PAR4) in the progression from colorectal polyps to cancer.Methods In this study,five groups of specimens were researched:primary colorectal cancer group (n =30),matched normal colorectal tissues group (taken 5 cm away from the tumour) (n =30),tubular adenoma group (n =30),villous adenoma group (n =20),and adenoma mixed group (n =30).The protein and mRNA levels of PAR3 and PAR4 were detected by immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) respectively.Results The positive expression rate of PAR3 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues was 73.3％,50.0％and 6.7％ respectively,with significant differences among them (P =0.009).The positive expression rate of PAR4 protein in these groups was 6.7％,35.0％ and 70.0％ respectively.FQ-PCR showed that the expression of PAR3 mRNA in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma was 0.787 ± 0.040,0.453 ± 0.023,0.410 ± 0.050,0.368 ±0.032 and 0.259 ± 0.017,P =0.0l 1;and that of PAR4 mRNA in these groups was 0.370 ± 0.301,10.384 ± 1.474,20.892 ± 4.485,36.311 ± 7.953,52.083 ± 12.550 (F =43.342,P =0.009,respectively).Conclusion These results suggested PAR3 and PAR4 may play a role in the development of colorectal cancer.

Objective To detect the expression and implication of Occludin protein in the normal mucosa and colorectal adenoma with different pathological types and different stages.Methods A total of 80 colorectal adenoma samples of different pathological type removed under endoscopy were collected,including 30 cases of tubular adenoma,30 cases of tubular villous adenoma,and 20 cases of villous adenoma.Furthermore,the samples also included 60 cases of colorectal carcinoma specimens and 40 cases of paracancerous normal tissue specimens that were cut off by general surgery.The immunohistochemistry was used to detect the expression of Occludin protein.Results The positive rate of Occludin expression in normal mucosa group,colorectal adenoma group and colorectal carcinoma group was 95.0％,88.7％ and 71.7％,respectively (P =0.003).The expression of Occludin is associated with the degree of differentiation.The positive rate of Occludin expression in tubular adenoma group,mixed pattern of adenoma group and villous adenoma group was 90.0％,86.7％ and 90.0％,respectively (P =0.901).Conclusion The expression of Occludin in colorectal carcinoma is reduced,and inhibited the occurrence and development of colorectal carcinoma.

Objective To explore the expression of human runt-related transcription factor 3 (RUNX3) in the progression from colorectal polyps to cancer.Methods In this study, five groups of specimens were researched: primary colorectal cancer group (n=30), matched normal colorectal tissues group (5 cm away from the tumour, n=30), tubular adenoma group (n=30), villous adenoma group (n=20), and adenoma mixed group (n=30).The protein and mRNA levels of RUNX3 were detected by immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) respectively.Results The positive expression rate of RUNX3 protein in normal colorectal mucosa, colorectal adenoma and colorectal carcinoma tissues was 96.7%, 73.8% and 46.7% respectively, with significant differentces among them (P=0.015).FQ-PCR showed that the expression of RUNX3 mRNA in normal colorectal mucosa, tubular adenoma, mixed pattern of adenoma, villous adenoma and colorectal carcinoma was 33.930 7±8.570 9, 16.459 1±3.383 7, 7.901 2±2.081 6, 3.714 6±1.710 9, and 0.871 5±0.523 1 respectively, F=47.859, P=0.021.Conclusion These results suggested RUNX3 may play a role in the development of colorectal cancer.

Objective To explore the expression of connexin 32 in normal colorectal mucosa,colorectal adenomas and colorectal carcinoma,and to analyze its relationships with different histological types of colorectal adenomas and colorectal carcinoma.Methods Immunohistochemistry was used to exzamine the location and expression of connnexin 32 in the colorectal tissues,colorectal carcinoma tissues and colorectal carcinoma tissues.Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect connexin 32 mRNA expression.Results There was a significant correla tion between up-regulated expression of connexin 32 and unfavorable variables,including nodal metastasis,Dukes stage and differentiation degree (P =0.032).There was no obvious difference in age and sex (P =0.061).The positive expression rate of connexin 32 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues was 66.7％,36.2％ and 5.0％ respectively,with significant differences among them (P =0.000).The positive expression rate of connexin 32 protein in tubular adeno ma,mixed pattern of adenoma and villous adenoma was 47.5％,35.0％ and 15.0％ respectively,with significant differences among them (P =0.047).FQ-PCR showed that the expression of connexin 32 gradually decreased in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous ade noma and colorectal carcinoma (P =0.006).Conclusion Connexin 32 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma.Detecting the expression of connexin 32 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma.

Objective To investigate the expression and significance of metastasis suppressor 1 (MTSS1) protein in colon adenoma and colon carcinoma.Methods Immunohistochemistry and fluorescence quantitative-polymerase chain reaction (PCR) were used to detect the location and expression of MTSS1 in the 20 normal colon mucosa,30 colon carcinoma,80 colon adenoma samples.Results The positive expression rate of MTSS1 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues were 100.0％,63.8％,6.7％,respectively,with significant differences among them (P =0.005).The positive expression rate of MTSS1 protein in tubular adenoma,mixed pattern of adenoma and villous adenoma were 82.5％,56.7％,35.0％,respectively,with significant differences among them(P =0.005).Fluorescence quantitative PCR showed that the MTSS1 mRNA expression are 36.58 ± 9.06,16.34±1.34,10.47±3.20,3.40±1.28,0.80±0.40.Conclusion MTSS1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma.Detecting the expression of MTSSI can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma.

Objective To investigate the expression and significance of Kissl protein in colon adenoma and colon carcinoma.Methods Immunohistochemistry and real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) were used to detect the location and expression of Kiss1 in the 20 normal colon mucosae,30 colon carcinomae,80 colon adenomae.Results The positive expression rate of Kiss1 protein in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues was 93.3％,58.8％ and 5.0％ respectively,with significant differences among them (P =0.005).The posi tive expression rate of Kiss1 protein in tubular adenoma,mixed pattern of adenoma and villous adenoma was 80.0％,66.7％ and 35.0％ respectively,with significant differences among them (P =0.005).FQ-PCR showed that the Kiss1 mRNA expression rate was 28.85 ±8.34,14.61 ± 1.65,7.58 ± 1.31,2.26 ± 1.56 and 0.50 ± 0.40 in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma,respectively.Conclusion Kiss1 is correlated with canceration of colorectal adenoma and the development of colorectal carcinoma.Detecting the expression of Kiss1 can evaluate the progression of colorectal adenoma and the development of colorectal carcinoma.

Objective To observe the expression and clinical significance of kang ai 1 (Kai1) in the process from the progression from colorectal polyps to cancer, invasion and metastasis.Methods Immunohistochemistry was used to examine the expression of Kai1 in the colorectal tissues, colorectal adenoma tissues and colorectal carcinoma tissues.Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect Kai1 mRNA expression.Results The expression of Kai1 protein was positive in 18 cases of colorectal carcinoma (45.00%), significantly lower than that in colorectal adenoma (91.25%) and colorectal tissues (100.00%), respectively, with significant differences among them (P=0.031).FQ-PCR showed that the expression of Kai1 mRNA in colorectal carcinoma, colorectal adenoma and normal colorectal mucosa was incresed by 2.038±0.933, 9.983±4.337 and 12.260±2.182 respectively (F=11.960, P=0.015).The expression of Kai1 protein and mRNA had a significant correlation with the lymph node metastasis of patients with colorectal carcinoma (P=0.011).ConclusionThe abnormal expression of Kai1 protein and mRNA is related with the carcinogenesis and the development of colorectal carcinoma.

Objective The aim of this study is to detect the expression levels of Tat interacting protein 30 (TIP30) in colorectal carcinoma,colorectal adenomas,normal colorectal mucosa,and analyze the relationships among different histological types of colorectal adenomas and colorectal carcinoma.Methods We collected 60 colorectal carcinoma tissues,40 normal colorectal mucosa tissues,80 colorectal adenomas.Immunohistochemistry and fluorescence quantitative polymerase chain reaction (PCR) were used to detect the expression of TIP30 in the tissues.Results The results of the immunohistochemistry were that the positive expression rates of TIP30 in normal colorectal mucosa,colorectal adenoma and colorectal carcinoma tissues were 95.0％,67.5％,30.0％,respectively,with significant differences among them (P =0.000).The positive expression rates of TIP30 in tubular adenoma,mixed pattern of adenoma,villous adenoma were 86.7％,66.7％,40.0％,respectively,also with significant differences among them (P =0.003).There was a significant correlation between reduced expression of TIP30 and unfavorable variables,including nodal metastasis (P =0.024),Dukes stage (P =0.008),differentiation degree (P =0.015).There was no obvious difference in age (P =0.815) and sex (P =0.955).Fluorescence quantitative PCR showed characterized amplification about TIP30.The expression of TIP30 mRNA in normal colorectal mucosa,tubular adenoma,mixed pattern of adenoma,villous adenoma and colorectal carcinoma were 25.135 ±1.474,18.701 ±1.596,12.412 ±1.677,6.579 ±0.795,2.137 ±0.535,respectively,F =250.931,P =0.015.Conclusion TIP30 may participate in the inhibition of the occurrence and development of colorectal adenoma and colorectal carcinoma.

近年来内镜隧道技术发展迅速，使得很多以前需要外科手术治疗的疾病进入内镜治疗的范畴。什么是内镜隧道技术？内镜隧道技术就是利用内镜在消化道黏膜下建立黏膜层与固有肌层间的一条通道，通过该通道进行黏膜层侧、固有肌层侧以及穿过固有肌层到消化管腔外进行诊疗的技术［1］。

Objective To examine the feasibility and safety of gastric submucosal tunnel dissection of gastric submucosal tumors (SMTs) by double tunnel and double flex endoscope. Methods Fifty patients with gastric SMTs detected by gastric endoscopy and endoscopic ultrasonography between January, 2012 and August, 2013 were enrolled in this study. Using carbon dioxide throughout the procedure, the mucous in the arc was incised along the margins of the lesion to separate the submucosa and create a tunnel. The exposed SMTs were resected completely and the mucosa was covered by endoscopic forceps followed by clipping of the incision. The complication, clinical outcomes, hospital stays and operation time were evaluated. Results Of the 50 lesions, 50 were located in the gastric fundus, 17 in the gastric antrum and 5 in the gastric body. The lesions were completely resected in all the patients. The diameter of the resected lesions ranged from 0.5 to 2.5 cm (mean 1.1±0.6 cm), and the operation lasted for 35.3 ± 16.2 min (range 23-76 min). In 5 cases (10%), perforation occurred during the operation and was closed by clipping the incision with endoclips after the lesion resection;these patients were discharged after conservative management. Intraoperative bleeding occurred in 16 cases and was successfully managed through endoscopic methods. No delayed postoperative bleeding or perforation occurred in these patients. None of the 48 patients followed up showed tumor recurrence at one year after the operation, and 2 patients were lost for follow up. Conclusion Endoscopic submucosal dissection of gastric SMTs is effective and safe using double tunnel and double flex endoscope.

Objective To evaluate the clinical value of preoperative mark with methylene blue for the submucosal tumor originating from the muscluaris propria around the cardia in submucosal tunnel.Meth-ods A total of 27 patients with cardiac tumors originating from muscularis propria diagnosed by endoscopy and endoscopic ultrasonography underwent endoscopic submucosal tunnel dissection from June 2011 to May 2014.Eighteen cases were marked by methylene blue,and 9 others were not.The operation time and the in-cidence of complications were compared between the two groups.Results All lesions were resected success-fully.The time of lesion location of non-mark group was 14.7 minutes(9-32 min),and that of mark group was 8.1 minutes(7-10 min).The incidence of subcutaneous emphysema of thorax and cervix of non-mark group was 2 /9(2 cases),and that of the mark group was 1 /18(1 case).The incidence of pneumoperitone-um of non-mark group was 1 /9(1 case),while that of the mark group was 2 /18(2 cases).There was no pneumothorax or mediastinal emphesema in all cases.Conclusion Marking with methylene blue before op-eration can shorten operation time effectively and lower incidence of complications.

OBJECTIVE:To summarize the experiences of resecting cardiac muscularis propria benign tumors though esophageal submucosal tunnel and examine the occurrence rate of complications.METHODS:A total of 17 cases of cardiac muscularis propria benign tumor as diagnosed by endoscopy and endoscopic ultrasonography underwent endoscopic submucosal tunnel dissection from January 2012 to October 2013. And the appearances of lesions and the operative complications were recorded and analyzed.RESULTS:All lesions were successfully resected with a mean diameter of 1.6 (0.8-3.5) cm. There was no recurrence. The mean operative duration was 43 (26-105) min. The appearances of lesions were regular (n = 5) and irregular (n = 12). There was no instance of severe bleeding or perforation. Resection of muscularis propria was performed for 3 cases. The complications included pneumoderm (n = 3) and pneumoperitoneum (n = 3). All the above cases were discharged after conservative treatment.CONCLUSION:Endoscopic submucosal tunnel dissection is safe, reliable, convenient and fast for cardiac muscularis propria tumors method.

Objective To investigate the effects of captlpril on type Ⅰ collagen mRNA expression of culured hepatic stellate cells (HSCs) in vivo Methods HSC-T6 rat HSCs were chosen as the study model of the activated HSCs.Cultured HSCs were randomized into control group,angiotensin Ⅱ (Ang Ⅱ)group (1 × 10-5,1 × 10-7 mol/L) and AngⅡ + captlpril group (1 × 10-5,1 × 10-7 mol/L).HSCs were harvested to detect the collagen Ⅰ mRNA expression by reverse transcription-polymerase chain reaction (RT-PCR).Results Type Ⅰ collagen gene expression levels in different groups of AngⅡ (1 × 10-5,1 × 10-7 mol/L) were 0.850 ± 0.107 and 0.620 ± 0.103 respectively,and that in control group was 0.438 ± 0.061.Type Ⅰ collagen gene expression levels in different groups of Ang Ⅱ + captlpril (1 ×10-5,1 ×10-7 mol/L) were 0.650 ±0.087 and 0.510 ±0.062.Conclusion AngⅡ can increase the type Ⅰ collage mRNA expression of HSCs,which was inhibited by captlpril.