小鼠皮肤移植模型是研究T淋巴细胞识别同种异体抗原机制的标准方法,亦是探讨免疫排斥反应及鉴定近交系小鼠的基本方法之一[1].根据移植皮片的来源不同,目前常用的小鼠皮肤移植方法有背-背法、耳-背法、尾-背法等[2].我们在参考相关文献的基础上,建立小鼠耳朵来源皮片的皮肤移植模型,现报告如下。

Objective To establish a model of kidney transplantation in mice more convenient. Methods Twenty C57BL/6 mice were used for kidney isografts. The end-to-side anastomosis was per-formed between the donor aortic cuff with renal artery and recipient aorta of abdomen, and the donor renal vein and recipient IVC. 2-3 stitches and 4-6 stitches were required for each side' s anastomosis. It didn't need to be tied at the end of the suture. The construct of ureter was modified with joining the donor ureter to the recipient bladder. Results Total operative time was (45.1±3.5) min. The success rate was 95%. All mice were observed for 12 weeks after operation. Serum creatinine level was in normal range. Body weight gain was observed. Renal histology was normal. Conclusion This model is easy and conven- ient. The anastomosis can be controlled to prevent thrombosis and bleeding. It is an ideal model.

>心血管疾病是终末期肾病(ESRD)患者最主要的并发症和死亡原因。临床研究显示高血磷、慢性炎性反应可能是ESRD患者心血管钙化的主要危险因素。本研究拟通过体外大鼠血管平滑肌细胞(RVSMC)的原代培养,探讨高磷和肿瘤坏死因子α(TNF-α)对RVSMC钙化的影响和

<正>因各种不完全抗体引起配血不合的报道较多[1-3],原因多由一种抗体引起,但因IgM型合并IgG型的不完全抗体引起的配血不合则较为少见。IgM抗-E在0.9%NaCl溶液(以下简称生理盐水)介质中即可检测到,但Rh系统中抗体引起配血不合多见于IgG抗-E、

Objective To isolate, culture and induce different subsets of dendritic cells from human peripheral blood mononuclear cells (PBMC). Methods PBMCs were prepared from buffy coats of peripheral blood by centrifugation through a Ficoll density gradient. Then dendritic cells were isolated using the specific monoclonal antibody magnetic microbeads. After adding different cytokines, cells were differentiated into different stages. Results The purity of dendritic cells was greater than 95% as assessed by flow cytometry. Mature denendritic cells expressed higher levels of HLA-DR, CD86, CD40 than immature dendritic cells. There were also morphological differences between two subsets of dendritic cells. Conclusion We have developed the methodology for isolating, culturing and inducing the dendritic cells of different stages in vitro.