Background Cataract is commonly observed in patients with primary angle-closure glaucoma; however, its underlying pathological mechanisms remain unclear. This study aimed to improve our knowledge on the pathological processes involved in primary angle-closure glaucoma (PACG) by identifying potential prognostic genes associated with cataract progression. Methods Thirty anterior capsular membrane samples were collected from PACG patients with cataracts and age-related cataracts. Differentially expressed genes (DEGs) between these two cohorts were analyzed using high-throughput sequencing. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to screen the DEGs, and potential prognostic markers and their coexpression network were then predicted by bioinformatic analyses. The DEGs were further validated by reverse transcription-quantitative polymerase chain reaction. Results A total of 399 DEGs were found to be specifically associated with cataracts development in PACG patients, among which 177 and 221 DEGs were upregulated and downregulated, respectively. STRING and Cytoscape network analyses revealed seven genes— CTGF , FOS , CAV1 , CYR61 , ICAM1 , EGR1 , and NR4A1 —that were remarkably enriched and mainly involved in the MAPK, PI3K/Akt, Toll-like receptor, and TNF signaling pathways. RT-qPCR-based validation further confirmed that the sequencing results were accurate and reliable. Conclusions Herein, we identified seven genes and their signaling pathways that may contribute to cataract progression in patients with high intraocular pressure. Taken together, our findings highlight new molecular mechanisms that may explain the high incidence of cataracts in PACG patients. In addition, the genes identified herein may represent new foundations for the development of therapeutic strategies for PACG with cataract.

目的:探究对白内障手术患者实施手术室综合护理干预的临床效果.方法:采取随机数表法将在医院行手术治疗的102例白内障患者分为两组,每组各51例,分别采取常规护理干预和综合护理干预,前者为对照组,后者为研究组,观察两组护理效果.结果:护理前,研究组SAS、SDS评分为(50.88±4.72)分、(53.45±5.09)分,与对照组(50.91±4.67)分、(53.51±5.12)分无差异(t=0.0323,P=0.9743、t=0.0594,P=0.9528>0.05);护理后,研究组SAS、SDS评分为(38.44±3.96)分、(41.42±4.15)分,低于对照组(46.61±4.33)分、(49.47±4.59)分,差异显著(t=9.9434、9.2904,P<0.05);研究组并发症发生率为3.9％(2/51),低于对照组19.6％(10/51),差异显著(χ2=11.885,P=0.001<0.05).研究组基础操作、服务态度、责任意识及风险处理能力评分分别为(91.24±3.45)分、(92.26±3.37)分、(91.87±3.41)分、(90.85±3.56)分,高于对照组(84.86±4.57)分、(83.74±4.76)、(84.41±4.62)分、(83.14±4.79)分,差异显著(t=7.9571、10.4326、9.2779、9.2259,P<0.05).结论:对接受手术治疗的白内障患者实施综合护理干预可改善其负面情绪,减少术后并发症出现,患者也会有更高的医疗满意度,值得大力推广和引用.

目的 探讨改良人工晶体安装方法在飞秒激光辅助白内障手术(FLACS)中的应用效果.方法 将2019年9～11月在陆军军医大学大坪医院行FLACS的80例患者随机分为对照组和观察组,每组40例.植入的人工晶体选用一片式两襻疏水性丙烯酸制成的可折叠、后房型人工晶体,对照组患者按照常规方法安装晶体,观察组患者按照改良后的方法安装晶体.观察并比较2组患者手术时间、晶体展开时间、术中晶体植入囊袋后襻的变化、术中人工晶体破损率、术后角膜水肿、术后晶体调位等情况.结果 观察组患者手术时间、晶体展开时间明显短于对照组,差异有极显著性统计学意义(P<0.01);观察组患者术中晶体植入囊袋后襻自动展开情况明显优于对照组,差异有极显著性统计学意义(P<0.01);观察组患者术中人工晶体破损率、术后角膜水肿、晶体调位与对照组比较,差异无统计学意义(P>0.05).结论 改良后的人工晶体安装方法能够促进FLACS患者人工晶体植入囊袋后前后襻的自动展开,减少手术时间,提高手术效率,降低术中意外发生率.

AIM: To discuss the influencing factors of interface operation in femtosecond laser assisted cataract surgery(FLACS). METHODS: From March 2016 to October 2017, 44 patients(44 eyes)were enrolled in this study who had adverse cases occurred in interface operation in femtosecond laser assisted cataract surgery. A questionnaire survey was conducted to find out the relevant factors. RESULTS: The patient's emotion, comfort and external environment can influence the process and safety of interface operation in femtosecond laser assisted cataract surgery. The positive detection rate of patients with state anxiety was 73%, with mild and moderate status anxiety, among which there was no statistical difference between male and female state(P>0.05). The patient's comfort level was 89% and the satisfaction rate was 96%. CONCLUSION: The emotional, comfort, and environmental factors of patients in femtosecond laser assisted cataract surgery may affect the process and safety of interface operations, effective nursing and management measures can be implemented to improve patient surgical coordination and comfort, and reduce intraoperative complications.

目的：总结视网膜脱离患者的院外护理干预方法。方法：对我院2014年9月～2015年3月的81例视网膜脱离患者81眼玻璃体切割手术后出院前进行系统的院外护理干预，包括用药、体位、随访、饮食、生活及心理指导等。结果：手术81眼，术后解剖性复位81眼，功能性复位78眼，成功率96．30％。术后随访1～3个月，光感3眼，手动2眼，指数6眼，视力0．01～0．09者16眼，0．1～0．2者42眼，≥0．2者12眼。结论：术后系统的院外护理干预，对确保手术有效性、巩固疗效及促进康复起着重要作用。

<正>斜视是眼科的常见病,手术矫正是治疗斜视的主要手段。在斜视手术中,手术医师需要通过遮盖试验反复调整缝线,最终达到斜肌的最佳调整度数。而手电筒是遮盖试验的必用工具,以往的传统方法有两种:一是由巡回护士持手电筒,听医