In this research, a reliable DNA extraction from different variety of Amorphophallus xiei was developed for RAPD analysis.The results revealed that the high quality DNA was extracted by the modified CTAB method and 8 random primers were used to identify the variety.The way of leaf dividing, the number of bulb and the strips of leafstalk in 9 varieties were analyzed.The results showed the Nei's similarity of coefficients were in the range of 0.440～0.800, the average Nei's similarity of coefficient was 0.714, the maximal similarity of coefficient was 0.008 between clone 1 and clone 8, the minimal Nei's similarity of coefficent was 0.440 between clone 1 and clone 3.These 9 varieties were clustered into 3 groups by UP-MGA analysis.The strips of leafstalk were suggested to be the main index for selected variety in Amorphophallus xiei.

Objective Erigeron breviscapus( Vant. ) Hand-Mazz. is a kind of important Chinese herbal medicine,its natural resource can’t meet the demand of market,so find a substitute from similar speices is an important way to resolve this problem. Methods Using the ITS sequence of Erigeron breviscapus( Vant. ) Hand-Mazz,some species of feverfew ,and other species to construct phylogenetic tree,then to find the difference of homology between the ITS phylogenetic tree of different genus or orders and traditional Phylogenesis,in order to quest the genetic background of Erigeron breviscapus( Vant. ) Hand-Mazz,and carry out some relative experiments or research. Results The phylogenesis in a genus based on ITS was reliable. Conclusion Using ITS to find a similar substitutable species is practical.

Chalcone isomerase is a key enzyme in flavone biosynthesis regulation of Erigeron breviscapus(vant) Hand Mazz.Isolation and cloning of functional genes concerning flavone biosynthesis is crucial for using gene transfer to regulate flavone metabolism in Erigeron bre-viscapus.In this paper,the chalcone isomerase gene(CHI) was cloned from Erigeron bre-viscapus via RT-PCR and RACE(rapid amplification of cDNA ends).The full-length cDNA of chalcone isomerase gene is 996 bp(GenBank Accession No.GU208823),containing a 594 bp open reading frame(ORF),encoding a 197 amino acid protein,and having a polyadenylation signal in its 3' untranslated region.The deduced protein molecular weight of CHI was 21.6 kD and its theoretical isoelectric point was 4.78.The CHI protein did not have trans-membrane domain and was hydrophilic.Its secondary structures were predominantly constructed by α-helic and random coils were the main component.The 3D model structure of the deduced Erigeron breviscapus protein was constructed similar to the CHI protein structure from Medicago sativa.According to variation characteristics in the N-end of CHI,we supposed that the special synthesis of breviscapine(scutellarin-7-O-glucuronide and apigenin-7-O-glucuronide) in Erigeron bre-viscapus may be concerned with the position of CHI in cell sub-structure and the assembled manner of enzymes in flavone biosynthesis.This research established the foundation for di-rectional change in flavone biosynthsis in Erigeron breviscapus(vant) Hand Mazz.

黄酮是生物体内具有重要功能的一类次生代谢产物,其含量与植物对环境的响应及功效都有明显的影响.本文就氮磷元素及光照、蔗糖浓度对黄酮代谢相关基因表达的影响进行了综述.