将16头种公猪随机分为试验组和对照组.试验组种公猪每天每头饲喂12.5g中草药添加剂-神农精粉,连用15天.结果表明:饲喂中草药添加剂神农精粉后8-12天、12-15天内精液量、精子密度、精子活力两组间差异极显著(P＜0.01),顶体异常率、精子畸形率两组间差异显著(P＜0,05),停止使用中草药添加剂神农精粉后10天内,精液量、精子密度、精子活力、顶体异常率两组间差异显著(P＜0,05),pH值无明显变化.

香豚猪原是我国少数民族地区珍贵的微型地方猪种,由于其特殊的生存环境和生理特点,限制了其扩繁,本试验研究采用中草药饲料添加剂饲养香豚母猪,结果表明中草药饲料添加剂对香豚母猪繁殖性能有很好的促进作用,与对照组相比,添加中药制剂试验组母猪的采食量增多,与空白对照组差异极显著(P≤0.01);中草药饲料添加剂试验组母猪的泌乳力与空白对照组差异极显著(P≤0.01);中草药饲料添加剂组香豚母猪产活仔数与空白对照组差异显著(P≤0.05),中草药饲料添加剂组与抗生组比空白对照组约提早1.5d发情,差异显著(P≤0.05);创造了很好的经济效益和社会效益.由此可见,因地制宜,采用中草药饲料添加剂对香豚猪扩繁和饲养是一可行性途径.

哺乳仔猪腹泻是当前众多猪场的棘手问题,是制约仔猪生长性能的一个重要制约瓶颈,因此对应用中药制剂来降低仔猪腹泻的研究意义重大.中兽医辨证施治从整体观念出发,以平衡阴阳、协调脏腑功能、健脾消食为治则,采用的方剂多以提高免疫、增强体质为主,对降低仔猪的腹泻率有一定的作用.中药对促进仔猪生长发育的配方主要原则是补脾益气、消食和胃[1],以此降低腹泻率.本研究采用复方中药制剂作为母猪的饲料添加剂,防治哺乳仔猪腹泻,并通过对仔猪相关血液生化指标的分析进行效果确定,以期为中药制剂替代抗生素,生产无药物残留、健康生态的动物食品开辟一条新的用药途径.

This research was conducted to clone and express the PB1_P protein of avian influenza virus H5N1 RNA polymerase and purify PB1_P recombinant protein with high concentration and its preliminary crystals,which could be useful for the further study of the function and structure of the influenza virus po-lymerase and the development of a new diagnostic kit.According to the sequence of polymerase basic protein 1 PB1 gene in GenBank,a pair of specific primers were designed.Polymerase domain(PB1_P) of avian influenza virus PB1 gene was amplified by RT-PCR,and inserted into pET-28a,then the constructed recombinant plasmid was analyzed.Then,the positive pET-28a/PB1_P plasmids were transformed into E.coli BL21(DE3)competent cell and induced for 4 h,at 37 ℃ by 0.5 mmol/L IPTG.The recombinant fusion protein PB1_P was successfully expressed and identified by SDS-PAGE and Western-blot and then purified by Ni-NTA His Bind Resins and column chromatography.After purification,the preliminary crystals of PB1_P recombinant protein was obtained by the hanging drop vapor diffusion method.The pET-28a/PB1_P recombinant plasmid was successfully reconstructed.Analysis of SDS-PAGE showed that the fusion protein PB1_P was successfully cloned and expressed.The result of Western-blot indicated that the recombinant had specially immunologic reactionogenicty with postive serum.The preliminary crystals of PB1_P recombinant protein was obtained after purification and crystallization screening.This study provides a sound basis for further study on biological function and of PB1 and the development of a new avian influenza virus H5N1 detection kit.