Objective To compare the anti-tuberculosis effects of four kinds of genes encoding the antigens in proliferation phase of M.tuberculosis in mouse tuberculosis (TB) model,and provide experimental basis for developing new TB immunotherapy agents.Methods Seventy female BALB/c mice were infected via the tail vein with 6.4× 105 CFUs of M.tuberculosis H37Rv,then randomly divided into 7 groups and treated as follow at the third day after infection:saline,plasmid vector pVAX1,M.vaccae vaccine,ag85a DNA,rv1291c DNA,rv1419 DNA and rv2223c DNA,which were injected intramuscularly 3 times at two-week intervals.The mice were sacrificed at two weeks after the final immunization.The lungs and spleens from the mice were taken and their pathological changes,weights and number of mycobacterial colony were examined.Data were expressed as means and standard deviations.Results The lung histopathological examination showed that the lesions were severe and extensive in saline group,lighter in plasmid vector pVAX1 group than those in saline group,slight and limited in the M.vaccae vaccine,ag85a DNA,rv1291c DNA,rv1419 DNA and rv2223c DNA groups with relatively clear and normal structure of alveoli.Compared with saline group,ag85a DNA group,rv1291c DNA group and rv1419 DNA group reduced the pulmonary bacterial loads respectively by 0.53 lg CFU,0.67 lg CFU and 0.41 lg CFU (x2 =27.07,P＜0.01);spleen bacterial loads respectively by 0.43 lg CFU (t＞3.004,P＜0.05),0.34 lg CFU (t＜3.004,P＞0.05),and 0.41 lg CFU (t＞3.004,P＜0.05);M.vaccae vaccine and rv2223c DNA groups also decreased lung and spleen bacterial loads,but there were not significant differences (t＜3.004,all P＜0.05).Conclusion M.tuberculosis rv1291c and rv1419 genes encoding the antigens in proliferation period had better irnmunotherapeutic effects on TB,which were similar to ag85a DNA in mice.Rv2223c DNA had not obvious immunotherapeutic effect.

目的 研究不同工艺制备的中药复方牛贝消核提取物的体外抑菌活性,为制备抗结核中成药制剂提供实验基础.方法 制备分别含50,100,200 g·L-1牛贝消核水提取物、醇提取物的改良罗氏培养基,并分别制备含1,10μg/ml异烟肼(Isonicotinic acid hydrazide,INH)和50,250 μg·ml-1利福平(Rifampicin,RFP)的改良罗氏培养基为对照,将3株耐多药结核分枝杆菌分离株、牛结核分枝杆菌和4株非结核分枝杆菌分别接种于含药的改良罗氏培养基中,置37℃培养4周,测定其最低抑菌浓度(MICs).结果 牛贝消核水提取物对这8株供试菌的MICs均为200 g·L-1;醇提取物对牛结核分枝杆菌和母牛分枝杆菌的MICs均为100 g·L-1,对3株耐多药结核分枝杆菌分离株、胞内、瘰疬、偶发分枝杆菌的MICs均为200 g·L-1.结论 牛贝消核水提取物和醇提取物对敏感或耐药的结核分枝杆菌和非结核分枝杆菌均有不同程度的抑制作用,醇提取物的抑菌活性比水提取物略强.

目的 研究中药复方牛贝消核提取物治疗结核病的相关靶标,阐明其作用机制.方法 9只小鼠分为正常组、结核模型组和牛贝消核治疗组,每组3只.结核模型组和牛贝消核治疗组经尾静脉注射5×105 CFU(菌落形成单位)的结核分枝杆菌H37Rv标准株的悬液感染7d后,牛贝消核治疗组用牛贝消核水提取物进行灌胃.治疗12周后,杀鼠采血至抗凝管,提取小鼠全血总RNA,反转录后与小鼠基因表达谱芯片杂交,应用IPA(Ingenuity Pathway Analysis)数据库分析、比较各组间的基因表达结果,以差异变化倍数≥2.0作为基因表达明显上调或下调的判断标准.结果 结核模型组和正常组基因表达谱比较共有差异表达基因5647条,其中2898条上调,2749条下调.结核模型组和牛贝消核治疗组比较共有差异表达基因7051条,3454条基因上调,其中涉及多个免疫相关因子;3597条基因下调,c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)信号通路明显抑制下调.结论 牛贝消核提取物具有明显的免疫调节作用,具有下调JNK信号通路、抑制炎症的作用.

通过查阅大量文献,研究中医药治疗小儿肺结核最新进展.对近10年内的国内外的中医药在治疗小儿肺结核相关文献进行阅读和整理,比较不同中医药在治疗小儿肺结核的差异,找到适合小儿肺结核治疗的方剂.目前各地医家,以小儿肺结核中医理论为基础,结合自身长期临床经验,创立各具特色方剂,使中医药治疗小儿肺结核病取得一定进展,但仍存在很多不足.中医药可以抑菌、杀菌及增强免疫功能,促进病灶组织的修复,改善相关临床症状,提高临床疗效,与西药连用可降低或消除抗痨药物的毒副作用.