Objective:To evaluate the clinical curative effect of NiuBeiXiaoHe prescription treatment of tuberculosis (TB).Methods:90 cases of TB patients were treated with NiuBeiXiaoHe prescription,in which 83 cases took decoction and 7 cases took powder.The TB patients were examined by chest X-ray and sputum smear after treatment for 3 to 4 months.Results:After treatment for 3 to 8 months,in the decoction group,46 cases (55.42％) were cured,29 cases (34.94％) had obvious effect,6 cases(7.23％) were effective,2 cases (2.41％) had no effect,the effective rate was 97.59％;in the powder group,5 cases (71.43％) were cured,2 cases (28.57％) had obvious effect,the effective rate was 100％.Conclusion:NiuBeiXiaoHe decoction and powder both have remarkable curative effect on TB.

目的 研究不同工艺制备的中药复方牛贝消核提取物的体外抑菌活性,为制备抗结核中成药制剂提供实验基础.方法 制备分别含50,100,200 g·L-1牛贝消核水提取物、醇提取物的改良罗氏培养基,并分别制备含1,10μg/ml异烟肼(Isonicotinic acid hydrazide,INH)和50,250 μg·ml-1利福平(Rifampicin,RFP)的改良罗氏培养基为对照,将3株耐多药结核分枝杆菌分离株、牛结核分枝杆菌和4株非结核分枝杆菌分别接种于含药的改良罗氏培养基中,置37℃培养4周,测定其最低抑菌浓度(MICs).结果 牛贝消核水提取物对这8株供试菌的MICs均为200 g·L-1;醇提取物对牛结核分枝杆菌和母牛分枝杆菌的MICs均为100 g·L-1,对3株耐多药结核分枝杆菌分离株、胞内、瘰疬、偶发分枝杆菌的MICs均为200 g·L-1.结论 牛贝消核水提取物和醇提取物对敏感或耐药的结核分枝杆菌和非结核分枝杆菌均有不同程度的抑制作用,醇提取物的抑菌活性比水提取物略强.

目的 研究中药复方牛贝消核提取物治疗结核病的相关靶标,阐明其作用机制.方法 9只小鼠分为正常组、结核模型组和牛贝消核治疗组,每组3只.结核模型组和牛贝消核治疗组经尾静脉注射5×105 CFU(菌落形成单位)的结核分枝杆菌H37Rv标准株的悬液感染7d后,牛贝消核治疗组用牛贝消核水提取物进行灌胃.治疗12周后,杀鼠采血至抗凝管,提取小鼠全血总RNA,反转录后与小鼠基因表达谱芯片杂交,应用IPA(Ingenuity Pathway Analysis)数据库分析、比较各组间的基因表达结果,以差异变化倍数≥2.0作为基因表达明显上调或下调的判断标准.结果 结核模型组和正常组基因表达谱比较共有差异表达基因5647条,其中2898条上调,2749条下调.结核模型组和牛贝消核治疗组比较共有差异表达基因7051条,3454条基因上调,其中涉及多个免疫相关因子;3597条基因下调,c-Jun氨基末端激酶(c-Jun N-terminal kinase,JNK)信号通路明显抑制下调.结论 牛贝消核提取物具有明显的免疫调节作用,具有下调JNK信号通路、抑制炎症的作用.