Objective To investigate the effect and mechanism of IL-15 converting CD4+CD25-T cells into CD4+CD25+ regulatory T cells(Tregs).Methods CD4+CD25-T cells were isolated from human peripheral blood mononuclear cells in 2 steps by magnetic cell sorting system.The purity rate of the sorted cells was measured by flow cytometry(FCM).CD4+CD25-T cells were divided into 4 groups: control group(without cytokines),IL-15 group(IL-15),antagonism I group(IL-15 and STAT5a blocking peptide was added at the first day) and Antagonism Ⅱ group(IL-15 was added at the first day,but STAT5a blocking peptide at the third day).FCM was used to detect the changes of CD4+CD25-T cells,the incorporation of -thymidine was used to evaluate the suppressive function of the IL-15-induced Tregs(iTregs) on the Teff and Western blot analysis was used to observe the expression of p-STAT5.Results Compared with CD4+CD25-T cells cultured without cytokines,IL-15 was able to promote CD25 and Foxp3 expression of CD4+CD25-T cells and IL-15-induced CD4+CD25+ regulatory T cells exerted weak suppressor activity.Addition of IL-15 up-regulated the expression of p-STAT5 in CD4+CD25-T cells significantly.After STAT5a blocking peptide was added,IL-15-induced CD25 and Foxp3 expression of CD4+CD25-T cells was significantly down-regulated.Conclusion IL-15 significantly converts naive CD4+ CD25-T cells into CD4+CD25+ regulatory T cells in vitro and up-regulated p-STAT5 may be involved in this process.

Objective To compare the successful rate and security of percutaneous renal biopsy under transient intravenous anesthesia and traditional local anesthesia in children. Methods Clinical and pathological data of children patients (less than 12 years old) undergoing ultrasound-guided renal biopsy with intravenous anesthesia from June 1, 2006 to May 30, 2007 in our department (Painless group) were compared with those of traditional local anaesthesia for renal biopsy from January 1, 1999 and May 30, 2006 (local anaesthesia group). In painless group, fentanyl citrate (50 μg for less than 60 kg in body weight, and 75 μg for those over 60 kg) were injected intravenously, then diprivan was injected intravenously till the end of lash reflex (usually 140-150 mg), and the puncture was performed when the child got complete narcosis. In local anaesthesia group, 2% lidocaine was applied for local anaesthesia, rapid puncture was performed when the children were asked to hold the breath. Successful rate (those getting more than 15 glomeruli) and the complications were observed. Results The punctures in painless group were done successfully with glomeruli number of 8 to 49 [mean (23.3±10.0)], and only 1 case got 8 glomeruli; while in local anesthesia group, the glomerulus number was 0-40 [mean (23.2±9.4)], with no difference between the 2 group. But in local anesthesia group, 2 biopsies were abandoned due to bad cooperation caused by supertension, and 2 cases obtained less than 15 glomeruli. Successful rate was 96.3% and 82.6% respectively in the 2 groups. The complications of anaesthesia in 2 groups: the analepsia time in painless group was 2-5 min, and respiratory depression took place in 1 case who got better soon after the oxygen intake was increased and the respiratory tract was unobstructed; 2 cases in local anesthesia group presented discomfort of chest distress, sweat, and fluster etc. which got better after rest. There was no difference in the incidence of gross hematuria and perirenal hematoma. Conclusion Compared with traditional renal biopsy in local anesthesia, transient intravenous anesthesia is safe and reliable to perform for renal biopsy in children.

<正>目的探讨老年肾脏病患者的病理形态学特点及其于临床症状之间的关系。方法选取我科2000-2005年间,年龄在60岁及60岁以上的老年肾脏病住院患者,共78例,其中男性患者52例,女性患者26例,男女比例为:2.55:1,所有患者均符合肾脏疾病诊断标准(不含肾移植

<正>目的通过全麻状态下无痛肾穿与既往局麻状态下肾穿比较,观察无痛肾穿的安全性、优越性,并对其并发症发生情况进行分析。方法对我院从2004年8月到2008年1月的1892例肾穿患者临床资料进行回顾性分析。其中从2006年5月开始行无痛肾穿共1092例,年龄5～78岁,儿

目的 回顾性总结1 096例肾活检的病理结果,并分析其类型.方法 对2000年1月～2005年4月间1 096例肾脏活检的病理类型进行统计和总结.结果 IgA肾病284例,系膜增生性肾小球肾炎253例,特发性膜性肾病92例,特发性FSGS 77例,硬化性肾炎66例,膜增生性肾炎41例,毛细血管内增生性肾炎15例,新月体肾炎7例,微小病变4例;狼疮性肾炎111例,紫癜性肾炎18例,糖尿病肾病11例,肾脏淀粉样变性9例,慢性间质性肾炎25例,急性肾小管坏死21例,急性间质性肾炎7例,高血压肾病21例,血管炎、先兆子痫、Alport综合征及Goodpasture综合征各1例.20例移植肾有12例为急性排斥病变,8例为慢性移植肾病.结论 原发性肾小球肾炎是最常见的肾小球疾病,原发性疾病主要以IgA肾病最为常见;继发性疾病仍以狼疮性肾炎为主.