Objective To study the protective effects of Ginkgolide B(GB) on celiac ganglion(CG) neurons induced by intestinal ischemia/reperfusion in guinea pig in vitro.Methods 30 guinea pigs were randomly divided into three groups: control group,ischemia/reperfusion(I/R) group,I/R+GB group(treated with GB 2.5×10-4 mol/L).The intestinal injury was induced by clamping the superiormesenteric artery for 30 min and reperfusion for 60 min.The intracellular bioelectric recording technique and HE staining were applied to monitor the protective effects of GB on celiac ganglion neurons.Results In this study,CG neurons of the ischemia/reperfusion group were injuried. The intracellular bioelectric recordings showed GB could improve and recovery some physiologic function of CG neurons,stabilized the potential of membrane.With the stimulation of depolarized square-wave,the action potential was observed in 70.1% (61/87) cells of normal group compared with 12%(3/25) cells of I/R group.Under the same condition,in I/R+GB group,42.4% (20/48) cells depolarized and the action potential was found in 31.2%(15/48) cells(P<0.05).In HE staining observation,after the perfusion with GB,the amount of CG neurons of the group was much more than that of I/R group (P<0.05).Conclusion GB is responsible for the protective effect on CG neurons which are induced by intestinal ischemia/reperfusion in guinea pigs in vitro.