Objective To study the sleep improvement function of DHA-PC. Methods The mice were randomly divided into control,vehicle,DHA + Lecithin( 60 + 200 mg / kg) and DHA-PC( 50,100,200 mg / kg) groups. Ten mice were enrolled in each group. The mice of control were administered with normal food,the vehicle group was orally given normal saline at the dosage of 0. 2 ml /10 g,while both DHA-PC and DHA + Lecithin were orally given corresponding drugs at the dosage of 0. 2 ml /10 g. All the groups were treated for 30 days except control group. The direct sleep-inducing test,the test of lengthening sleep time induced by pentobarbital sodium,the test of pentobarbital sodium subthreshold-hypnosis and the test of barbital sodium sleep latency were conducted to observe the inductive effect of DHA-PC. Results Neither the effect on mice body mass nor directly-induced sleep was observed. DHA-PC( 50,100,and 200 mg / kg) could prolong sleep time to( 56. 2 ± 13. 7),( 57. 9 ± 25. 4) and( 64. 1 ± 18. 4) min,respectively,compared to vehicle( 32. 9 ± 10. 8) min( P 0. 05). DHA + Lecithin could not prolong sleep time( 38. 6 ± 11. 7) min compared to( 32. 9 ± 10. 8) min of vehicle. There was significant difference compared with DHA-PC at the dosage of 200 mg / kg( 64. 1 ± 18. 4) min( P 0. 05). DHA-PC( 200 mg/kg) enhanced pentobarbital sodium subthreshold-hypnosis( 70%) compared to vehicle( 10%)( P 0. 05),so did DHA + Lecithin( 60%) compared to vehicle( 10%)( P 0. 05). Both DHA-PC( 200 mg / kg) [( 22. 9 ± 4. 1) min ]and DHA + Lecithin [( 19. 5 ± 2. 7) min ]could shorten sleep latency compared to vehicle( 31. 3 ± 6. 9) min( P 0. 01),and the sleep latency of DHA + Lecithin( 19. 5 ± 2. 7) min was shorter than that of DHA-PC( 50,100 mg / kg). Conclusion DHA-PC has some effect some sleep improvement in mice.

目的 观察硫辛酰维格列汀对2型糖尿病大鼠血糖的影响.方法 选用雄性Wistar大鼠,高脂高糖饲料喂养4周后小剂量注射链脲佐菌素建立2型糖尿病模型,随机将大鼠分为正常对照组、糖尿病模型组、胰岛素糖尿病组、硫辛酰维格列汀糖尿病组.分别测定给药后0、0.5、1、2、3、4、5、6、7、8、9、10、12、24h的血糖变化.结果 注射链脲佐菌素72h后造模大鼠血糖均明显高于注射前(P＜0.05)和正常对照组(P＜0.05).与正常对照组和糖尿病模型组相比,胰岛素糖尿病组在注射胰岛素后0.5～4h,血糖变化差异有统计学意义(P＜0.05);硫辛酰维格列汀糖尿病组在灌胃给药3～10h,血糖变化差异有统计学意义(P＜0.05).结论 硫辛酰维格列汀和胰岛素有不同程度的降血糖作用,相比胰岛素,硫辛酰维格列汀降糖作用更加持久.

硫化氢(H2S)作为第三种气体信号分子在生命活动中具有特殊的作用。在心血管系统中H2S主要由胱硫醚-γ-裂解酶(CSE)催化生成,CSE在心肌和主动脉上高表达。H2S可直接作用于KATP通道实现对血管的调节作用。现已证明H2S与多种心血管疾病关系密切。

Objective To explore the effect of chloratum kalium( KCl) on the production of H_2S and ultra- microstructure of pulmonary artery smooth muscle cells( PASMCs). Methods Rat's cultured PASMCs were randomly di- vided into control group and KCl groups at different time poits. Each group contained 4 samples ( V =5ml,D = 1～2× 10~5/L). The KCl was added into PASMCs at the concentration of 50mol/L. 5ml of each sample was sucked at the 5th, 15th and 30th minute. The concentration of sulfion was measured by sensitive sulfion electrode and the cultured PASMCs were observed by electron microscope. Results Compared with the control group, the concentration of sulfion was re- spectively increased by 162. 72% ( P 0. 05 ) ,253. 68% ( P 0. 01 ) ,63. 23% ( P 0. 05 ) for PASMCs in each KCl group;the number of rough endoplasmic reticulum( RER) in KCl group is much more than that in control group. Conclu- sion KCl can sublimate the concentration of sulfion in rat's PASMCs.

Objective To study the survival and infiltration condition of cord blood derived-T lymphocytes in mice and its therapeutic action for erythroleukemic(EL) mice;and to provide the experimental and theoretical basis for studying the biological characteristics and anti-leukemia effects of cord blood-derived T cells.Methods Fourty-eight BALB/c mice were randomly divided into three groups,the control group(CG),the experimental group 1(EG 1)and the experimental group 2(EG 2).Cord blood derived-T lymphocytes were separated and prepared,and transplanted it into EL mice via the tail vein or peritoneal injection after being signed to their group.This was done in order to observe the survival time and infiltrate condition of the cord blood-derived T cells in mice and its effect on the morphosis of EL mice spleen by immunofluorescence(IMF),immunohistochemistry(IMC) and transmission electron microscope(TEM).Results Most activated cells were CD3~+ T lymphocytes by PHA,and the positive rate was(83.42±1.26)%;human CD3~+ cells and Brdu~+ cells were detected in peripheral blood,spleens and liver of all mice at the 3rd and 7th day after cord blood derived-T lymphocytes were transplanting;CD25~+ cells could be detected in the liver and spleen of EL mice in both experimental groups;and the average survival time of EL mice in the two experimental groups was(27.25±7.06) days and(24.74±2.93) days respectively(this was statistical significance compared to control group,P0.05).The results of Hoechst 33258 staining showed: 1.Nuclear fluorescence was uniform in the control group and there were few apoptotic cells;2.In the experimental group,some of the leukemia cells appeared to have morphological changes consistent with apoptosis; chromatin had a high degree of cohesion;dense concentration,or splited into massives,edge was smooth and clear;Under TEM,leukemic cells infiltrate was generally observed in the spleens of the control group mice.Parts of the leukemic cells showed typical apoptosis morphological changes in the experimental groups: nuclear chromatin concentration and edge accumulation was seen;nuclei fragmentation and formation of apoptotic bodies;the expansion of RER,and the vacuolar or medullar degeneration of mitochondria could be found.Conclusion The cord blood-derived T lymphocytes could be activated in vivo,and transferred to the site of leukemia,and educed relevant function,thus having some therapeutic action for leukemia.