The development of high-strength, durable, and bio-based adhesives for harsh conditions like underwater or extreme temperatures remains a significant challenge. Inspired by the wet-adhesion chemistry of mussels, this study presents a bioinspired oil composite gel adhesive based on renewable plant-derived materials. The adhesive was synthesized via catechol-modified modified epoxidized soybean oil (ESO) intertwined with poly(acrylic acid) (PAA) through intensive hydrogen bonding and chain entanglement, affording rapid gelation and high cohesive strength, which integrated mussel-inspired adhesion with oil hydrophobicity. The resulted PAA-ESO-DHCA (PED) adhesive demonstrated a remarkable dry adhesion up to 24.30 MPa and moderate long-term water tolerance, retaining 2.83 MPa after 6 months of water immersion. Another 1 kg weight hanging test underwater could persist for over one year. Notably, 78% of adhesion could be recovered after re-curing process, which further enhanced the water resistance in subsequent cycles. It also showed strong tolerance to other harsh conditions, such as extreme high/low temperatures and various solutions. Therefore, this study provides an effective strategy for producing high-performance, long durability and bio-based adhesives suitable for various demands.

β-Hydroxybutyrylation (Kbhb) is a novel posttranslational modification (PTM) mediated by β-hydroxybutyrate (BHB). BHB, the core product of ketogenic metabolism, serves as its direct precursor and substrate. As a hub connecting energy metabolism and the epigenetic network, Kbhb exerts bidirectional regulatory effects on abnormal tumour metabolism, cardiovascular and cerebrovascular diseases, immune regulation, and other processes. Furthermore, Kbhb is not limited to histones; it is also widely present in nonhistones and influences various biological processes, such as protein stability, metabolic and energy homeostasis regulation, pathogen virulence regulation, transcriptional regulation, and signal transduction. This review summarizes the research progress in the field of Kbhb, including the inducers of Kbhb (ketogenic diet), prediction methods for modification sites (KbhbXG, pFunK, SLAM, iBhb-Lys), regulatory elements of modification (regulatory enzymes such as ENL and SIRT6, and protein substrates), mechanisms of action in cancer (e.g., mTOR signalling pathway, cGAS-STING signalling pathway), mechanisms of action in immune-related signalling pathways and immune-active components regulation, research progress on histone and nonhistone Kbhb (e.g., Bcl6, P53, STAT1, UvSlt2), and novel therapeutic strategies for diseases based on Kbhb modification (metabolic regulation and targeted therapy), providing new insights for targeted therapy for cancer and other diseases.

The solar-driven semi-artificial biohybrid system incorporates semiconductor materials with microbial metabolism, affording an innovative strategy for antibiotic degradation via photocatalysis. In this study, the sulfur metabolic pathway of biological cells was rationally engineered using CRISPR-Cas9 and Cre-loxP site-specific gene editing systems, successfully achieving intracellular accumulation of sulfide up to 552.84 ppm. Based on this capability, In(Ⅲ) was adsorbed in situ onto the cell surface, leading to the self-assembly of photosensitive In2S3 nanoparticles (NPs). The resulting inorganic-biological hybrid system of In2S3-cell exhibited a broad-spectrum light-harvesting capability with an ideal optical bandgap of 1.96 eV. Photoelectrochemical analysis confirmed the charge transfer process and the semiconductor biointerface based regeneration mechanism of redox cofactors in the cytosol. Photogenerated electrons directly form ROS for tetracycline oxidation, and are transferred to cells for enhance the regeneration of intracellular reducing cofactors. This light-driven photocatalytic biohybrid system enabled efficient tetracycline degradation of over 98% within 4 h and demonstrated excellent stability over consecutive cycles. Transcriptomic analysis identified key genes involved in solar energy capture, electron transport, and metabolic regulation, elucidating their functional roles in biomanufacturing processes and photocatalytic degradation. This study presents a bottom-up paradigm for the biotic-abiotic system from electronic and molecular perspectives to develop efficient and sustainable technologies for antibiotic remediation and solar energy conversion.

Orchestration of lipid production, storage and mobilization is vital for cellular and systemic homeostasis1,2. Dysfunctional plasma lipid control represents the major risk factor for cardiometabolic diseases-the leading cause of human mortality3,4. Within the cellular landscape, the endoplasmic reticulum (ER) is the central hub of lipid synthesis and secretion, particularly in metabolically active hepatocytes in the liver or enterocytes in the gut5,6. Initially assembled in the ER lumen, lipid-ferrying lipoproteins necessitate the cross-membrane transfer of both neutral and phospholipids onto the lumenal apolipoprotein B (APOB), in a poorly defined process7-10. Here we show that the ER protein CLCC1 regulates cellular lipid partition and, consequently, systemic lipid homeostasis by participating in trans-bilayer equilibration of phospholipids. CLCC1 partners with the phospholipid scramblase TMEM41B11,12 to recognize imbalanced bilayers and promote lipid scrambling, thereby supporting lipoprotein biogenesis and the subsequent bulk lipid transport. Loss of CLCC1 or TMEM41B leads to the emergence of giant lumenal lipid droplets enclosed by imbalanced ER bilayers and, consequently, accelerated pathogenesis of metabolic-dysfunction-associated liver steatohepatitis. The results reveal that phospholipid scrambling at the ER is essential for establishing a dynamic equilibrium. Considering the requirement of trans-bilayer phospholipid equilibration in numerous biological processes, ranging from catabolic autophagy to viral infection13-16, we anticipate that future work will elucidate a homeostatic control mechanism intrinsic to ER function in lipid biogenesis and distribution.

Poor thermal stability of Rhizopus oryzae lipase (ROL) has long limited its industrial applicability. In this study, we systematically investigated the underlying mechanism for its inactivation and correspondingly developed an effective stabilization strategy. Kinetic analyses revealed that ROL thermal inactivation process followed a two-step model, demonstrating its inactivation proceeds a distinct intermediate state. Various spectroscopic characterizations further suggested that activity loss was closely associated with the progressive unfolding of the enzyme, as evidenced by the disruption of secondary structures and a pronounced increase in fluorescence intensity resulting from the exposure of hydrophobic clusters. To enhance its thermostability, based on the above-discovered mechanism, a compound stabilizer system was developed and further optimized via a combination of Plackett-Burman design and response surface methodology. The obtained optimal formula comprised of 35.94 % (m/v) glucose, 34.82 % (m/v) sorbitol, and 2.50 mol/L NaCl, which could effectively preserve 97.7 % of the initial activity after 3 h of incubation at 60 °C, in contrast to only 29.5 % residual activity of the control. Thus, this study elucidated the underlying thermal inactivation mechanism for ROL and developed a practical and efficient stabilization strategy with potential prospect for industrial application.

Achieving robust and durable adhesion under harsh conditions remains a fundamental challenge for practical adhesive materials. Here, a novel in vitro mussel-inspired adhesion system was developed that mimics the natural mussel adhesion process from peptide biosynthesis to coacervate assembly. Adhesive peptides rich in 3,4-dihydroxy-L-phenylalanine (DOPA) and lysine were produced through a two-step enzymatic catalysis involving papain-catalyzed polymerization followed by tyrosinase-mediated hydroxylation. Systematically screened reductants could effectively suppress DOPA oxidation during catalysis and storage. A new immobilization strategy was established to covalently attach tyrosinase onto functionalized diatom frustules via click chemistry, achieves enhanced activity recovery (116.9%), rapid separation from the catalyzed system, and high conversion of tyrosine to DOPA (86.7%), and retained similar to 80% activity after six cycles. The same protocol also immobilized papain with satisfactory efficiency. The peptides were grafted onto a polyglutamic acid backbone, and subsequently conducted DOPA-induced phase separation, yielding an innovative mussel-mimicking coacervate. The coacervate exhibits an ultrahigh shear adhesive strength (up to 31.8 MPa) and stable adhesion in air, water, oil, organic solvents, and extreme temperature range (-196 to 150 degrees C), as well as robust wet tissue adhesion for wound closure. This study provides a promising opportunity for developing high-performance mussel-inspired adhesives.

Photosynthetic semiconductor biohybrids, which combine the light-harvesting capacity of semiconductors and catalytic activity of whole-cell microorganisms, show substantial potential for advancing bioremediation technology. However, few yeast-based biohybrid systems for pollutant removal were reported. In this study, we have constructed a whole-cell biohybrid system based on Yarrowia lipolytica featuring in situ synthesized biocompatible cadmium sulfide (CdS) nanoparticles (NPs) for the photocatalytic reduction of hexavalent chromium [Cr(VI)] under UV irradiation. The integration of these CdS NPs onto the surface of modified Y. lipolytica cells endowed the system with excellent photocatalytic performance, achieving 100% Cr(VI) reduction within 2 h. The system exhibited a higher kinetic constant (0.03 min-1). In the trapping experiments, the reactive oxygen species (ROS) generated photochemically, specifically the superoxide anion (•O2-), which were identified as crucial mediators that facilitate the reduction of Cr(VI). The enhanced activity of the Y. lipolytica-CdS biohybrid was attributed to efficient electron transfer. Additionally, through transcriptome analysis, we found that the differentially expressed genes are associated with membrane transport, oxidation-reduction process, energy metabolism, and electron transfer. This whole-cell biohybrid catalytic strategy holds promise as an innovative approach for the reduction of Cr(VI) and has the potential to enhance our understanding of the interactions among light, inorganic material, and microorganisms.

Acquired sensorineural hearing loss (SNHL) is primarily caused by the damage or loss of hair cells (HCs), induced by factors such as noise exposure and ototoxic drugs. However, clinical treatments for SNHL remain limited. Here, the role of the apoptosis-inducing gene Cidea in SNHL is investigated. It is initially observed that Cidea expression is specifically increased in neomycin-damaged HCs at both the protein and mRNA levels. To further explore its role, Cidea knockout (Cidea-/-) mice are obtained, and it is found that the absence of Cidea effectively alleviates HC apoptosis caused by neomycin treatment and noise exposure in vivo. Moreover, a novel therapeutic strategy for SNHL has been developed by delivering CRISPR/SlugCas9-HF via AAV to edit Cidea, and this approach significantly reduced HC loss induced by both neomycin and noise exposure. These findings suggest that Cidea may serve as a promising target for the prevention of neomycin- and noise-induced SNHL in clinical settings.

Adipose tissue dysfunction in obesity is a major global public health risk, contributing to insulin resistance and chronic diseases such as diabetes and cardiovascular disorders. Here, we identify a dominant c.37A>G p.(Arg13Gly) variant in the long isoform of CIDEC (CIDEC-L), a key regulator of lipid droplet (LD) size, as the underlying cause of familial obesity. Affected individuals display marked subcutaneous fat accumulation in white adipose tissue (WAT), elevated fat content in brown adipose tissue (BAT) and insulin resistance. Accordingly, patient-derived iPSCs differentiated into white adipocytes exhibit accelerated LD growth, a phenotype mirrored by CIDEC-LR13G overexpression. Mechanistically, we find that the p.Arg13Gly variant disrupts the N-terminal structural order of CIDEC-L, shifting its phase separation properties to enable, rather than restrict, lipid exchange through condensation plates between LDs. Notably, knock-in mice with the analogous Cidec-L p.(Arg10Gly) mutation recapitulate the human BAT hypertrophy and exhibit impaired thermogenesis. These findings establish the CIDEC-LR13G variant as the first example of a dominantly inherited monogenic obesity driven by a dysfunctional adipocyte LD protein, revealing a critical role for CIDEC-L in restraining fat accumulation and maintaining metabolic health. ### Competing Interest Statement The authors have declared no competing interest. ### Funding Statement F. P. is a recipient of a long-term European Molecular Biology Organization (EMBO) postdoc fellowship and a short-term EMBO travel fellowship. Her research is supported by the Singapore Ministry of Health National Medical Research Council under its Young Individual Research Grant scheme (Project ID MOH-000549-01) and A*STAR under its Career Development Award (Project number C210112002). L. J. T. is supported by the A*STAR Career Development Fund (CDF C243512024). We thank Prof. Patrick TAN and the Genome Institute of Singapore (GIS) for supporting the costs associated with the travel and clinical phenotyping of two patients in Singapore. Research in L.P. laboratory was supported by the National Key R&D Program of China (2024YFA1802802) and the National Natural Science Foundation of China (92357302). Funding by the National Institutes of Health (DK130852 and DK116056 to M.P.C) and by the Isadore and Fannie Foxman endowed Chair in Medical Research at the University of Massachusetts Chan Medical School to M.P.C. is gratefully acknowledged. (SR/MED/GENT/16/01). B.R. is a fellow of the Branco Weiss Foundation (Switzerland) and an EMBO Young Investigator (Europe). The research reported in this publication was supported by funding from King Abdullah University of Science and Technology (KAUST) and from GIS at A*STAR (Singapore). ### Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes The details of the IRB/oversight body that provided approval or exemption for the research described are given below: Institutional Review Boards of the Agency for Science, Technology and Research in Singapore and King Abdullah University of Science and Technology in Saudi Arabia; as well as the Domain Specific Review Board of the Singapore National Healthcare Group gave ethical approval for this work I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable. Yes All data produced in the present study are available upon reasonable request to the authors

Lipid droplets are dynamic organelles whose size and number signify their role in energy. However, owing to cellular heterogeneity and technological limitations, the relationship between the lipolytic ability and lipid droplet morphology is unclear. Here, we developed a live-cell imaging assay using geometric analysis to quantify cellular lipolysis at a single organelle level, designated imaging lipolysis. Using imaging lipolysis and super-resolution imaging, we found that lipolysis is controlled by both lipase-accessible lipid droplet surface area and lipase activity. Moreover, lipid droplet fusion regulatory proteins CLSTN3β/CIDEs promote lipolysis by increasing the total lipid droplet surface area-to-volume ratio in biophysical regulation. We further identified that brown adipocytes exhibit more efficient lipolysis due to higher lipase activity and a larger lipid droplet surface area-to-volume ratio compared to white adipocytes. Taken together, imaging lipolysis generally enabled single-cell lipase activity measurement and revealed a mechanistic basis for energy-generating brown adipocytes to enforce a multilocular phenotype for lipolysis.

CircRNAs are closely related to ferroptosis in gastric cancer cells; however, the mechanism by which circRNAs regulate ferroptosis in gastric carcinogenesis remains unknown. CircRNA-encoded novel peptides are functional products translated from the open reading frames (ORFs) within circular RNAs, demonstrating that circRNAs not only serve as non-coding regulators but also have the capacity to encode biologically active peptides. Compared with noncancerous cells, cancer cells have greater iron requirements, and ferroptosis occurs in response to radiotherapy, chemotherapy, and immunotherapy; therefore, ferroptosis activation may be a potential strategy to overcome the shortcomings of conventional cancer therapy. A mouse model of ferroptosis in gastric cancer was constructed, and a bioinformatics analysis was performed to analyze and characterize the circRNAs involved in ferroptosis in gastric cancer. The inhibitory effect of hsa_circ_0002301 on ferroptosis in tumors was confirmed both in vitro and in vivo. The presence and expression of HECTD1-463aa were verified using mass spectrometry, protein blotting, and immunofluorescence staining. The molecular mechanism of hsa_circ_0002301 was investigated using mass spectrometry and immunoprecipitation. We designed and synthesized antibodies specific for the small protein HECTD1-463aa encoded by hsa_circ_0002301 to verify its presence and purified HECTD1-463aa by constructing hsa_circ_0002301 overexpression vectors with FLAG tags and used liquid chromatography–tandem mass spectrometry (LC‒MS/MS) to detect the characterized peptides. In addition, HECTD1 binding to HECTD1-463aa was identified by immunoprecipitation (Co-IP) and mass spectrometry. We found that HECTD1-463aa inhibited HECTD1-mediated GPX4 ubiquitination by binding to HECTD1, an important regulator of cell death in ferroptotic cancer cells. hsa_circ_0002301 competitively inhibits the degradation of the GPX4 protein by HECTD1 through the encoded proteins HECTD1-463aa and HECTD1 to affect the ferroptosis level in gastric cancer cells.

The biological effects of magnetic fields (MFs) have been studied and applied in medicine over the past four decades. However, the influence of high-intensity pulse magnetic fields (HI-PMFs), theorized to exert even stronger biological effects, is rarely reported. Herein, a study was conducted to investigate the biological effects of 2.5 T HI-PMF on the model organism Escherichia coli and its corresponding physiological alterations. After being treated by HI-PMF, a notable increase was observed in its intracellular NADH/NAD+ ratio, coupled with an improved cell survival rate. Transcriptome analysis revealed significant upregulation of genes related to glucose metabolism. Subsequent experiments confirmed that if the initial intracellular glucose level was relatively high and markedly decreased after being treated with HI-PMF, the cell density would significantly rise, owing to the alleviated inhibition of cell division. On the contrary, a lower initial intracellular glucose level led to cell death under HI-PMF. Furthermore, reactive oxygen species (ROS) production was proved to be the main cause attributed to the above phenomena. Therefore, our study suggests that HI-PMF treatment promotes ROS production, enhances cellular glucose metabolism, and consequently influences cell division and survival rate according to the initial level of intracellular glucose.

Lignin-based nanofibers (LNFs) were fabricated via electrospinning using a mixture of lignin and polyvinyl alcohol (PVA) as the spinning solution. Next, the LNFs were sequentially subjected to homogeneous shearing, freeze-drying, in-situ thermal cross-linking, and thermochemical vapor deposition involving treatment with organosilanes to obtain ultralight, mechanically robust, thermal-insulated, and superhydrophobic LNF aerogels (LNFAs). Careful adjustment of the LNF content aided in precise control and optimization of the network structure, density, and porosity of the obtained LNFAs, which ultimately affected their compressive strength and thermal conductivity (25.46 ± 1.32 to 31.06 ± 0.09 mW⋅m-1⋅K-1). Moreover, the lignin content in LNFs also affected the nanofiber diameters, thereby regulating the compressive strength of LNFAs (3.40 ± 0.17 to 4.91 ± 0.16 kPa). The LNFAs with the lignin/PVA ratio of 2.5 and the LNF content of 0.7 g (LNFAs-2.5) possessed the density of 8.88 ± 0.13 mg⋅cm-3, porosity of 99.31 ± 0.01 %, and compressive strength of 4.91 ± 0.16 kPa under a compression strain of 60 %, and it still maintained good composition structures supported even after 150 compression and rebound cycles. The LNFAs-2.5 possessed favorable properties of high-water resistance with contact angle of 150.4° and low thermal conductivity, making synthetic warmth retention materials superior to down feathers.

Molecular property prediction is crucial for drug discovery in biopharmaceuticals since it helps identify promising compounds, optimizing the efficacy of developing new therapies. Despite its importance, existing deep learning-based methods for this task are often incongruous with fundamental chemical properties. Here we show that an unsupervised pretraining approach, Molecular Motif Learning (MotiL), learns molecular representations that preserve both whole-molecule structure and motif-level information directly from native molecular graphs. MotiL produces representations that group small molecules sharing a common core structure (i.e., scaffold) and proteins with related three-dimensional structures and functions. We evaluated MotiL on at least 16 molecule benchmarks, and uncovered that it captures analogous graph representations not only for small molecules with the same scaffold but also for protein macromolecules with similar structures and overlapping chemical functions such as tRNA binding. These informative representations empower MotiL to surpass the accuracy of state-of-the-art contrastive or predictive methods in the prediction of molecular properties like blood brain barrier permeability.

To explore the safety and feasibility of the modified approach for accessing the superior mesenteric artery (SMA) in total laparoscopic radical resection for right colon cancer. This single-center retrospective study included 107 patients who underwent total laparoscopic radical resection of right colon cancer at The First Affiliated Hospital of Wannan Medical College between August 2022 and December 2023. 53 patients were in the modified SMA approach (modified group) and 54 patients were in the traditional SMA approach (control group). The control group and modified group underwent total laparoscopic radical resection of right colon cancer, and the following baseline and pathological characteristics of the two groups were compared: intraoperative condition, postoperative recovery, and postoperative complications. Our modified surgical method was to isolate the mesocolon using a cranial(the ligament of Treitz) -to- caudal(the pedicle of ileocolic) pathway and the orderly ligation of blood vessels in the SMA. There was no statistically significant difference in the baseline characteristics or pathological data between the two groups. Compared with the traditional SMA approach, the modified SMA approach had a shorter surgical time(P < 0.001) and vascular dissection time (P < 0.001) and less intraoperative blood loss (P = 0.000). There was no statistically significant difference in the number of total harvested lymph nodes or positive harvested lymph nodes between the two groups of patients (P > 0.05); There was no statistically significant difference in postoperative hospital stay, time to first flatus, time to pull out drainage tube and drainage between the two groups of patients (P > 0.05), and there was no statistically significant difference in the incidence of complications between the two groups of patients (P > 0.05). The modified SMA approach in totally laparoscopic radical resection for right colon cancer can shorten the surgical and vascular dissection time, reduce intraoperative bleeding and reduce the surgical difficulty and intraoperative risk of the SMA approach for right colon cancer. In clinical practice, its safety and feasibility are relatively high, and it is worth promoting. The study was approved by the Ethics Committee of The First Affiliated Hospital of Wannan Medical College and registered with the China Clinical Trials Registry (ChiCTR2300075919, Date of Registration:2023-09-19- retrospective registration) http://www.chictr.org.cn/index.aspx .

Protein materials, valued for their good biocompatibility, versatility, and designability, are sought as innovative adhesives to address the environmental and health hazards caused by traditional adhesives. Herein, inspired by mussel foot protein 5 and elastin, a novel adhesive protein, M5EP, was designed and efficiently expressed in Escherichia coli BL21(DE3). It could be activated into mature protein M5EPm to obtain adsorption and adhesion capabilities on various interfaces via tyrosinase catalysis, exhibiting adhesion strengths exceeding 2 MPa under dry conditions and 0.2 MPa under humid conditions. Meanwhile, M5EP and M5EPm both had significant antibacterial activity against E. coli and Staphylococcus aureus. Additionally, a favorable in vitro platelet coagulation property and biocompatibility of this bioinspired adhesive have been confirmed, suggesting its promising potential both within industrial sectors and in the realm of biomedical applications.

Recently, transition-metal carbides (TMCs) have been developed as good catalyst candidates for oxidation desulfurization (ODS) reactions. However, improving their stability in strong oxidizing media remains a challenge. Herein, a W2C catalyst protected by spherical N-doped carbon was constructed in-situ through a facile one-step thermal annealing process using phosphotungstic acid and zinc phthalocyanine micro- sphere (ZnPcMS) as the W and carbon sources, respectively. During annealing, the spherical N-doped carbon matrix (SNC) was simultaneously derived from ZnPcMS and combined closely with the in-situ formed W2C, preventing W2C from oxidation. The W2C/SNC catalyst system, when combined with H2O2 as the oxidant, exhibits remarkable ODS performance. Desulfurization of model fuel oils can be achieved with 100 % efficiency within 25 min with a small H2O2 to S molar ratio of 3. Moreover, W2C/ SNC possesses superior reusability. A non-free radical oxidation mechanism is suggested for the ODS reaction based on radical scavenging tests. The as-prepared W2C/SNC microspheres could serve as potential effective ODS catalysts. (c) 2025 The Society of Powder Technology Japan. Published by Elsevier BV and The Society of Powder Technology Japan. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

IntroductionStrategies for preventing high glycolysis in tumour cells are urgently needed. CircRNAs (circRNAs) play important roles in glycolysis. However, the mechanism underlying the effects of hsa_circ_0001756 in gastric cancer (GC) remains unclear.MethodsIn this study, we detected the expression of hsa_circ_0001756 in GC tissues and cells using quantitative real-time polymerase chain reaction (qRT PCR). Construct a silencing and overexpression vector to validate the role of hsa_circ_0001756 in GC. Pulldown and RIP experiments were conducted to verify the identification of miRNA and protein binding to hsa_circ_0001756.ResultsThe expression level of hsa_circ_0001756 in GC tissues and cells is significantly upregulated. The expression level of hsa_circ_0001756 is closely related to TNM stage and tumour size in patients with GC. The proliferation and migration of hsa_circ_0001756-expressing cells in vitro were assessed by functional experiments. Hsa_circ_0001756 was found to not only promote the expression and stability of PGK1 by binding with polypyrimidine tract-binding protein 1 (PTBP1) but also promote glycolysis through the miR-185-3P/PGK1 pathway. We found that the regulatory relationships of competing endogenous RNA (ceRNA) and RNA-binding proteins (RBPs) with hsa_circ_0001756may affect glycolysis in GC.ConclusionThis study provides a theoretical basis for designing drugs that target molecules related to energy metabolism in tumours and provides a new strategy for the clinical treatment of GC.

Climate change driven by rising atmospheric CO2 levels underscores the urgent need for sustainable energy solutions. This study investigates the dual potential of CO2 as a primary carbon source and acetate as an intermediate to simultaneously mitigate atmospheric CO2 levels and generate bioelectricity using microbial fuel cells (MFCs). A synthetic microbial co-culture was developed, combining Clostridium ljungdahlii for CO2 sequestration and Shewanella oneidensis MR-1 for bioelectricity production. To optimize MFC performance, S. oneidensis was modularly engineered to enhance acetate metabolism and electron transfer efficiency. Key modifications included upregulating ATP synthesis, introducing an ATP-independent acetate metabolic pathway, increasing NADH availability, and optimizing pili-based artificial conductive nanowires. These advancements achieved a maximum cell density (OD600 = 0.611), a record output voltage of 351.3 mV, and a record power density of 94.9 mW/m2 using acetate as the substrate. Furthermore, a two-stage biocatalytic system utilizing CO2 as the primary carbon source yielded an output voltage of 209.3 mV and a power density of 65.0 mW/m2. These results highlight the potential of engineered microbial co-culture for efficient CO2-based bioelectricity generation, offering a scalable and sustainable pathway toward carbon-neutral energy production.