Activation of the Ras signaling pathway promotes the growth of malignant human glioblastoma multiforme (GBM). Mutations in Ras are rare in GBM, elevated levels of activated Ras are prevalently observed in GBM. However, the potential mechanism of how Ras is activated in GBM remains unclear. In this study, we screened a new interacted protein of Ras, PHLDA1. Our findings confirmed that PHLDA1 acted as an oncogene and promoted glioma progression and recurrence. We demonstrated that PHLDA1 was upregulated in GBM tissues and cells. PHLDA1 overexpression promoted cell proliferation and tumor growth. In terms of mechanism, PHLDA1 promoted cell proliferation by regulating Ras/Raf/Mek/Erk signaling pathway. Moreover, Src promotes GTPase activity of Ras via tyrosine 32 phosphorylation. PHLDA1 and Src competed for binding with Ras, inhibiting Ras phosphorylation by Src and rescuing Ras activity. This study may provide a new idea of the molecular mechanism underlying glioma progression and a novel potential therapeutic target for comprehensive glioblastoma treatment.

目的 探究基于微信平台的延续性护理对异位妊娠患者认知度及自护能力的影响.方法 选取2018年7月至2019年4月期间郑州大学第一附属医院收治的75例异位妊娠患者为研究对象,按随机数字表法将研究对象分为对照组(采用常规延续性护理,n=37)和研究组(采用基于微信平台的延续性护理,n=38).评估两组疾病认知程度、自护能力及护理满意度.结果 研究组疾病知识知晓率高于对照组(97.37％ vs 83.78,P＜0.05).干预前,两组护理自我护理能力测定量表(ESCA)评分无统计学差异(P＞0.05);护理干预2个月后两组ESCA评分均升高,且研究组ESCA评分显著高于对照组(P＜0.05).研究组护理满意度高于对照组(97.37％ vs 81.08,P＜0.05).结论 基于微信平台的延续性护理能显著提高异位妊娠患者认知度及自护能力,提升患者护理满意度,具有临床应用价值.

目的 探讨综合护理措施应用于妇科单孔腹腔镜手术的效果.方法 将接受妇科单孔腹腔镜手术治疗的56例患者纳为研究对象,根据护理方法的不同分成常规组(n=28)和综合组(n=28).常规组采取常规护理措施,综合组则采用综合性护理措施.采用焦虑自评量表(SAS)、抑郁自评量表(SDS)评价两组患者护理前后焦虑、抑郁情况,比较两组的手术相关指标、并发症情况、护理满意度评分.结果 与护理前相比,护理后两组SAS、SDS评分明显降低,且综合组SAS、SDS评分显著低于常规组,差异均有统计学意义(均P＜0.05).综合组患者的手术时间、卧床时间、术后首次肛门排气时间、住院时间均显著短于常规组,差异均有统计学意义(均P＜0.05).两组术后并发症发生率比较(14.29％ vs.32.14％),差异无统计学意义(P＞0.05).综合组患者的护理满意度评分显著高于常规组[(93.24±27.66)分 vs.(78.74±22.36)分],差异有统计学意义(P＜0.05).结论 对行妇科单孔腹腔镜手术患者实施综合护理措施可有效缓解其焦虑、抑郁情绪,缩短手术时间、住院时间,且护理满意度评分较高,值得推广应用.

目的 探讨晚期头颈部肿瘤患者灵性照顾需求的影响因素,并讨论安宁疗护护理策略.方法 选取2019年6—12月医院收治的晚期头颈部肿瘤患者为调查对象,收集患者的临床资料,采用中文版灵性护理需求量表评价晚期头颈部肿瘤患者的灵性照顾需求,采用单因素和多元线性回归分析晚期头颈部肿瘤患者灵性照顾需求的影响因素.结果 本研究共发放问卷160份,收回有效问卷154份.154例晚期头颈部肿瘤患者灵性照顾需求平均得分34.80±8.33分.单因素分析显示,性别、年龄、配偶情况、工作状态得分比较,差异无统计学意义(P>0.05);有宗教信仰、文化程度低、病程长、对死亡恐惧程度大的晚期头颈部肿瘤患者灵性照顾需求得分高于无宗教信仰、文化程度高、病程短、对死亡恐惧程度小的患者得分比较,差异有统计学意义(P<0.05).多元线性回归分析结果显示,宗教信仰、文化程度、病程、对死亡恐惧程度是晚期头颈部肿瘤患者灵性照顾需求的影响因素(P<0.05).结论 晚期头颈部肿瘤患者灵性照顾需求受文化程度、有无宗教信仰、病程、对死亡恐惧程度的影响,临床应给予晚期头颈部肿瘤患者合理的安宁疗护舒适护理以提高其生命质量.

TiO2 thin films were deposited by the energy filtrating magnetron sputtering (EFMS) technique and the traditional direct current magnetron sputtering (DMS) technique. The influence of the filtering electrode mesh number on the structure and optical properties of TiO2 thin films was investigated. The structure, surface morphology and optical properties were characterized by XRD, SEM and ellipsometric spectroscopy, respectively. Results show that the TiO2 thin films deposited by the DMS and EFMS techniques at the same deposition parameters are composed of the anatase phase exclusively. TiO2 thin films deposited at lower deposition rate by the EFMS technique have lower crystallinity, smaller particle size and smoother surface. With increasing the mesh number, the refractive index, extinction coefficient and optical band gap are larger.

利用自主改进后的能量过滤磁控溅射(energy filtering magnetron sputtering,EFMS)技术制备Te薄膜,研究了沉积温度对薄膜结晶特性、表面形貌以及光学性能的影响.研究结果表明所制备的薄膜为纯Te膜.在100℃时制备出的薄膜结晶较好,表面颗粒较大且比较均匀,200℃时薄膜表面形貌发生改变,由颗粒状变为交错分布的棒状结构.200℃时制备的薄膜在红外波段的消光系数和折射率相对较小.室温制备的薄膜的平均透过率为9.1％;200℃制备的薄膜的平均透过率最大为35.4％.

A specially designed magnetron sputtering technique, the energy-filtering magnetron sputtering (EFMS) technique, was utilized to deposit aluminum nitride (AlN) thin films on Si (100) substrates. Improvements in the crystallization properties, surface morphology and optical properties of the films were studied. The results indicate that EFMS can be a useful technique for preparing high-quality thin films, which will be helpful in enabling potential applications of AlN thin film based microelectronic, optoelectronic and surface acoustic wave devices.

目的 制备具备良好光催化性能的Cu2O/TiO2叠层复合薄膜.方法 利用直流磁控溅射技术(DMS)和能量过滤直流磁控溅射技术(EFMS)在玻璃基底上制备Cu2O/TiO2叠层复合薄膜,利用扫描电镜(SEM)、X射线衍射(XRD)、椭偏仪和光催化测试系统表征和分析了薄膜的表面形貌、结构、透射率和光催化性能.结果 DMS技术和EFMS技术制备的TiO2和Cu2O薄膜都有良好的结晶特性,其中TiO2为单一的锐钛矿结构.相对于DMS技术制备的Cu2O薄膜,EFMS样品中的Cu2O薄膜的衍射峰较弱,而且衍射峰的宽度变宽,衍射曲线比较平滑.薄膜表面较平整,颗粒均匀,较细小,边界明显.DMS和EFMS两种技术制备的薄膜的平均晶粒直径分别为15.4 nm和10.8 nm.透射光谱测试结果表明,EFMS技术制备的复合薄膜平均透射率较大,在350～800 nm范围内,平均透射率为0.388,DMS薄膜的值为0.343.对罗丹明B(RhB)的光催化降解结果表明,EFMS技术制备的薄膜的降解速率为?0.00411,大于DMS技术制备的薄膜的降解速率?0.00334.结论 EFMS技术制备的Cu2O/TiO2叠层复合薄膜对罗丹明B具有较大的光催化降解速率.

磁控溅射是一种广泛应用的薄膜制备技术.能量过滤磁控溅射是对磁控溅射技术加以改进后的一种薄膜制备技术.通过改变能量过滤磁控溅射技术过滤电极网孔大小制备TiO2薄膜,研究了网孔目数对薄膜物相结构、表面形貌以及光学性质的影响,并与磁控溅射技术制备的TiO2薄膜进行了对比研究.结果表明:(1)能量过滤磁控溅射技术较磁控溅射技术所制备薄膜的结构均匀性更加优异,薄膜的透射率由86.5％增大到91.4％、波长500 nm处折射率由1.61增加到1.68、波长300 nm处消光系数由0.42降低到0.12;(2)能量过滤磁控溅射技术过滤电极网孔目数不同时薄膜的结晶性能、表面粗糙度以及光学性质均存在差异,且随网孔目数的增加呈周期性变化;(3)过滤电极网孔目数为8目时,薄膜的结晶性能较好、薄膜较为均匀、透射率较高,为91.4％.

TiO2thin films were fabricated by the direct current magnetron sputtering(DMS)technique and energy filtrating magnetron sputtering(EFMS)technique on the glass substrate.Single and double anti-reflective layers were designed by the TFC membrane system design software and the parameters were determined.Single and double TiO 2coatings were prepared on the bases of the optimized results.The coatings were characterized by SEM, ellipsometer, contact angle tester and photochemical catalyst test system.Results show that the optimized coating has good anti-reflective property.The average transmittivity at 400-800 nm for the single and double layers are 0.845 and 0.891 respectively.The contact angles becomes from 61.5°to 28°and 8°when the samples are irradiated by the xenon lamp for 30 minutes.The coatings have good self cleaning performance and the degradation rates of RhB under the UV-light are -0.0019 min-1and -0.0034 min-1.Single and double coatings with single TiO 2have both anti-reflective and self-cleaning properties.The double layers composed of matched layers with high and low refractive indices have smoother transmission curve, better photocatalytic degradation property and hydrophilicity.

使用直流磁控溅射设备,不同压强下在玻璃衬底上制备了系列TiN薄膜.采用紫外-可见光分光光度仪测试了样品的光学性能,采用四探针法测量了样品的方块电阻.研究了溅射沉积过程中压强对TiN薄膜光学性能的影响.结果表明,随着反应压强的增大,TiN薄膜在可见光区透射率增强,反射率降低;薄膜厚度减小,方块电阻增大,中远红外光区的反射率降低.

通过改变溅射功率,使用直流磁控溅射设备,在玻璃衬底上制备了系列TiO2薄膜.分别用扫描电子显微镜、X射线衍射仪和椭偏光谱仪测试了TiO2薄膜的形貌、结构及厚度.使用紫外-可见分光光度计测试了TiO2薄膜的光学性能.使用TiO2薄膜降解罗丹明B溶液测试了其光催化活性.研究结果表明:溅射功率增大,薄膜的透射谱及吸收谱在紫外出现明显的"红移"现象,光学带隙从3.51 eV减小到3.40 eV.适当提高溅射功率有利于提高TiO2薄膜的光催化活性,当溅射功率为213 W时,薄膜的光催化性能最好.

The TiO2 thin films were deposited to improve the specific surface area of the films and im-prove the photocatalytic efficiency .The TiO2 thin films and double-layer TiO2 were deposited by DC mag-netron sputtering (DMS) technique and energy filtering magnetron sputtering (EFMS) technique on the basis of DC magnetron sputtering technique .The structure , surface morphology , optical properties , and photocatalytic properties of the films were characterized with X-ray diffraction , scanning electron micros-copy, spectroscopic ellipsometer sand UV-spectrophotometer .The results showed that double-layer TiO2 films deposited by the EFMS technique had more smooth surfaces , smaller grains, higher band gap, and the photo-catalytic efficiency was 2.4 times of the TiO2 thin films.

The uniformity of microstructures and optical properties of the TiO2 thin films,deposited by DC magnetron sputtering (DMS) and by energy filtering magnetron sputtering (EFMS) on glass substrates,was characterized with X-ray diffraction,scanning electron microscopy and ellipsometric spectroscopy.The results show that when it comes to the uniformity of the TiO2 coatings,EFMS outperforms the conventional DMS,particularly in large-area film growth.For example,the advantages of the EFMS TiO2 coating over the DMS films include the higher uniformity,better crystallinity,smaller grain-size,smoother surface,bigger transmittance,and lower refractive index,etc.Possible mechanisms responsible for the better uniformity of the EFMS TiO2 coatings were also tentatively discussed.We suggest that EFMS film deposition technique be of much technological interest in growth of high quality,large area TiO2 coatings on industrial scale.

The TiO2 thin films were deposited by DC and energy filtering magnetron sputtering (EFMS) on glass substrate,respectively.The impact of the mesh-size of the energy filtering grid on the microstructures and photocatalytic properties of the TiO2 films was investigated with X-ray diffraction,scanning electron microscopy and UV-spectroscopy.The results show that depending on the mesh-size,the energy filtering significantly improved the quality of DC magnetron sputtered (DMS)TiO2 coatings.The energy filtering markedly reduced the grain-size,decreased the surface roughness,improved the uniformity and increased the photocatalytic efficiency of the DC magnetron sputtered TiO2 coatings,because energy filtering effectively prevents the high energy secondary electrons from damaging the substrate and film growth.After UV irradiation for 2h in degrading rhodamine-B (RhB) solution,the TiO2 coatings,grown by EFMS,displayed the photocatalytic efficiency 1.5 times higher than that synthesized by DMS.

The work aims to study preparing conditions and influencing factors of anatase TiO2 film of high refractive index at low temperature. Direct current magnetron sputtering (DCMS) technique and improved energy filtering magnetron sputtering (EFMS) tech-nique were used to prepare TiO2 film. Effects of process parameters of DCMS technology on TiO2 film were studied in orthogonal ex-perimental method, optimal preparation conditions of anatase TiO2 film of high refractive index at low temperature was determined. TiO2 films were deposited by taking advantage of EFMS technique under the optimal preparation conditions, and films prepared by two techniques were compared as well. Microstructure of TiO2 film was characterized with X-ray diffractometer and Raman spectrometer, surface morphology was observed with scanning electron microscope and optical property was determined and fit with spectroscopic ellipsometer. The TiO2 films prepared by DCMS and EFMS techniques had excellent single anatase structure at 100℃. Porosity of TiO2 prepared by EFMS technique was 4.7%, refractive index at 550 nm was 2.47 and average crystal size was 12.5 nm. Optical band-gap of the TiO2 films deposited by the DCMS and EFMS techniques was 3.37 eV and 3.08 eV, respectively. TiO2 films can be prepared at low temperature by DCMS and EFMS techniques. Films prepared by the latter feature in smaller porosity, high refractive indexes, fine and well-distributed grain and large optical bandgap.

Resveratrol (RES) plays a critical role in the fate of cells and longevity of animals via activation of the sirtuins1 (SIRT1) gene. In the present study, we intend to investigate whether RES could promote the self-renewal and neural-lineage differentiation in human umbilical cord derived MSCs (hUC-MSCs) in vitro at concentrations ranging from 0.1 to 10 μM, and whether it exerts the effects by modulating the SIRT1 signaling. Herein, we demonstrated that RES at the concentrations of 0.1, 1 and 2.5 μM could promote cell viability and proliferation, mitigate senescence and induce expression of SIRT1 and Proliferating Cell Nuclear Antigen (PCNA) while inhibit the expression of p53 and p16. However, the effects were reversed by 5 and 10 μM of RES. Furthermore, RES could promote neural differentiation in a dose-dependent manner as evidenced by morphological changes and expression of neural markers (Nestin, βIII-tubulin and NSE), as well as pro-neural transcription factors Neurogenin (Ngn)1, Ngn2 and Mash1. Taken together, RES exerts a dosage-dependent effect on the self-renewal and neural differentiation of hUC-MSCs via SIRT1 signaling. The current study provides a new strategy to regulate the fate of hUC-MSCs and suggests a more favorable in vitro cell culture conditions for hUC-MSCs-based therapies for some intractable neurological disorders.

ZrMgMo3 O12 powders with negative thermal expansion were synthesized by solid-state reaction method. Spark plasma sintering ( SPS) technique was used to prepare ZrMgMo3 O12 ceramic. The influ-ences of structure, surface morphology and sintering temperature of ZrMgMo3 O12 on the properties of ther-mal expansion and thermal conductivity were discussed. The results showed: the sample sintered at the temperature of 150℃ had the highest relative density more than 97%. From room temperature to 600℃, the thermal expansion coefficient and the thermal conductivity were -4. 0 × 10-6/K and 1. 05~1. 3 W/(m·K), respectively. Hence, ZrMgMo3 O12 ceramic with high relative density, low thermal expansion coefficient and thermal conductivity could be prepared by SPS technique.

采用化学共沉淀法制备陶瓷Zr2P2WO12 (ZWP)和金属Ni复合材料粉体,烧结过程中还原剂柠檬酸将金属Ni2+还原.粉体中ZWP粒径100～200 nm,金属Ni粒径50～100 nm,且混合均匀.复合材料块体的制备采用冷压和放电等离子体烧结(SPS)两种方法,结果表明SPS制备的样品具有更好的块体致密度,可达84％以上.研究表明,通过调节Ni离子的掺杂比例可制备出热膨胀系数从负值到正值的可控热膨胀系数复合材料.当Zr2P2WO12/Ni的摩尔比为5∶5时,制备出的材料为近零膨胀复合材料.

Stem cell transplantation represents a promising therapy for central nervous system injuries, but its application to Alzheimer’s disease (AD) is still limited and the potential mechanism for cognition improvement remains to be elucidated. In the present study, we used Tg2576 mice which express AD-like pathological forms of amyloid precursor protein (APP) to investigate the effects of human umbilical cord mesenchymal stem cells (hUC-MSCs) intravenous transplantation on AD mice. Interestingly, hUC-MSCs transplantation significantly ameliorated cognitive function of AD mice without altering Aβ levels in hippocampus. Remarkably, hUC-MSCs transplantation reduced oxidative stress in hippocampus of AD mice by decreasing the level of malondialdehyde (MDA), increasing the level of nitric oxide (NO), enhancing the activity of superoxide dismutase (SOD) and neuronal nitric oxide synthase (nNOS). The mechanisms underlying the improved cognitive function may be linked to hippocampal neurogenesis and an up-regulation of neuronal synaptic plasticity related proteins levels including silent information regulator 1 (Sirt1), brain-derived neurotrophic factor (BDNF) and synaptophysin (SYN). Taken together, our findings suggest that hUC-MSCs can improve cognition of AD mice by decreasing oxidative stress and promoting hippocampal neurogenesis. These results suggest that modulating hUC-MSCs to generate excess neuroprotective factors could provide a viable therapy to treat AD.