对高等医学院校学生的培养,不仅要注重扎实的基本理论知识和实践技能,还要注重学生科学素质的培养,以期培养学生具有良好的再学习和发现新问题、认识新问题和解决新问题的能力。基础医学是医学生长远发展的基石,基础医学实验课不仅是学生学习和研究的重要手段,也是培养学生科学素质的良好摇篮。作者以病理生理学实验教学实践为例,在学生科学兴趣、科学精神、科学思维和科学研究方法培养等方面,进行了一些探索。

Objective To evaluate the effect of EGF on the growth of corneal fibroblasts.Methods The corneal fibroblasts of rabbit were cultured respectively in nutrient medium with different concentrations of EGF.Trypan blue staining test for cell viability was carried out after 24,48 and 72 hours.Results EGF at the concentration of 0.1, 1.0 mg/L had positive effect on the growth of fibroblasts at 48 and 72 hours(P0.05), but had no stimulating effect at 24 hours.EGF at concentration of 0.01 mg/L had no obvious effect on the growth of fibroblasts(P0.05).Conclusion EGF with certain range of concentration can increase the growth of corneal fibroblasts of rabbit.

目的用心率变异性的谱分析方法,探讨电针肾俞穴对自主神经功能的调整作用.方法将9只新西兰家兔静脉麻醉,用疏密波电针肾俞穴20min.将电针前后的呼吸节律与心电波RR间期同步输入微机,并用自回归模型进行谱分析.实验数据用配对t检验法处理.结果电针后家兔的心率减慢(P＜0.001)、心率变异性增大(P＜0.05)、低频峰面积增加(P＜0.01)、高频峰面积减少(P＞0.05)、两峰面积比增大(P＜0.05).结论电针肾俞穴可使心血管系统的活力增强,心交感活动显著加强,交感-迷走的均衡发生较大变化,自主神经功能得到良性调整.