目的 评价卡马西平片在中国健康受试者的生物等效性.方法 用单剂量、单中心、随机、开放、四周期自身交叉试验设计.空腹状态下46例、餐后状态下24例健康受试者,随机分为2组,每周期每组单次口服卡马西平受试制剂或参比制剂0.1 g,采用验证的液质联用法(HPLC-MS/MS)测定健康人血浆中卡马西平的浓度,应用Phoenix WinNonlin软件(Pharsight Corporation 7.0版本)进行非房室模型药代动力学参数的估算分析,计算主要药代动力学参数,其余关于描述性与推断性统计分析采用SAS 9.4版本统计软件进行统计分析.结果 受试者服用受试制剂和参比制剂后,药代动力学参数如下,空腹组:Cmax分别为1912.15和1850.11 ng·mL-1;AUC0-t分别为80775.69和81884.12 h·ng·mL-1.2种制剂的Cmax、AUC0-t的几何均值比值相对应的90％置信区间分别为97.76％～100.21％,101.29％～105.89％.餐后组:Cmax分别为1611.37和1619.17 ng·mL-1;AUC0-t分别为77426.90和78070.12 h·ng·mL-1.2种制剂的Cmax、AUC0-t的几何均值比值相对应的90％置信区间分别为97.74％～101.27％,97.80％～102.16％.结论 2种卡马西平片在中国健康受试者体内具有生物等效性.

当前全国高校近年来明显加大了综合设计性实验的比例,同济大学也对病原生物学实验进行教学改革探索.阐明了病原生物学实验课程建设的目的,论述了开展病原生物学实验课程建设的必要性和背景,讨论了病原生物学实验课程建设的内容与结构.通过综合性设计性实验教学实践,总结了病原生物学综合设计性实验教学的特色与效果.分析了病原生物学综合设计性实验教学开展过程中遇到的相关问题.

人体寄生虫学标本在实验教学中一直发挥着重要的作用.为了使其得到更好的利用,同济大学医学院病原生物学教研室将人体寄生虫大体标本、玻片标本数字化,助力实验教学,逐渐完成线上线下相结合的实验教学体系构建,既促进了学科建设,又提高了教学质量.

为完善本科课程体系建设,配合本科人才培养方案充分有效的实施,病原学实验整合后根据课程特点和学生需求在实验项目设计和实验教学方法两方面开展探索,旨在改变验证性实验过多、学生学习主动性欠缺的状况,着力培养新时期下医学生的创新实践能力.

Objective To evaluate PBL teaching results in medical virology experiment course. Methods Medical students of class 2011 were randomized assigned into PBL group (n=45, 20 five-academic-year students and 25 seven-academic year students) and control group (n=63, 38 five-academic-year students and 25 seven-academic year students). Teaching effectiveness was evaluated by scores of experiment, medical virology examination and final examination. PBL group was surveyed with questionnaire. SPSS statistical software was used and t test was employed to do analysis. Results Scores in medical virology examination were higher in seven-academic-year students in PBL group than in control group and there was no difference in other two examination scores between the two groups. Survey showed that interest motivation, case analysis, experiment design and knowledge grasp-ing in PBL group were highly satisfied. However, evidence-based and generalization ability among seven-academic-year students as well as autonomous learning ability and ability to deduce material among five-academic-year students were not completed satisfied. Conclusions PBL with public health events in medical virology experiment course can develop students' intrinsic motivation, knowledge grasping and public health awareness. However, PBL methods should be adjusted to suit different academic students.

In line with the teaching reform,in experimental teaching,the teaching methods,course content,and examination of experimental courses were reformed.After learning the basic skills,case study was used to guide the students to analyze and solve problems,determine pathogens and select sensitive drugs.Practice proves that the students speak highly of these reforms.The experimental courses were changed from passive to active,the students' enthusiasm and interest for learning were stimulated.At the same time,the reform played a promoting role in the improvement of the quality of teachers.

This article introduces our practice of building a multi-dimensional practical teaching platform of pathogen biology relying on experimental teaching center;including developing visual teaching materials;compiling the experiment guidance;using multimedia courseware;setting up a specimen room;offering animal experiments and clinical practice.It has achieved satisfactory results.

Objective To investigate the role of G protein-coupled receptor kinase 2 (GRK2) in epidermal growth factor (EGF)-induced cAMP accumulation and the underlying mechanisms. Methods cAMP assay was used to measure cAMP accumulation in HEK293 cells transfected with GRK2 or β-Gal as control upon EGF stimulation. Coimmunopre-cipitation method was used to study the interaction between GRK2 and EGFR. Results First, intracellular second messenger cAMP accumulation was enhanced upon EGF stimulation in HEK293 cells. The cAMP accumulation induced by EGF stimulation was about 30%-40% of forskolin-induced cAMP accumulation. EGF-induced cAMP accumulation was greatly inhibited when the cells were transfected with GRK2 compared with control cells transfected with (-Gal (P 0.05),suggesting the negative regulation of EGF-induced cAMP accumulation by GRK2. Second, immunoprecipitation experiments showed that EGF stimulated GRK2 binding to EGFR complex in HEK293 cells in a time-dependent manner, suggesting EGF-induced GRK2-EGFR complex formation. Conclusion Overall, these data indicate that in HEK293 cells overexpressions of GRK2 and EGFR exist,GRK2 plays a negative modulatory role in EGF-induced cAMP accumulation, and this may involve the interaction between GRK2 and EGFR.

Objective To evaluate the effect of EGF on the growth of corneal fibroblasts.Methods The corneal fibroblasts of rabbit were cultured respectively in nutrient medium with different concentrations of EGF.Trypan blue staining test for cell viability was carried out after 24,48 and 72 hours.Results EGF at the concentration of 0.1, 1.0 mg/L had positive effect on the growth of fibroblasts at 48 and 72 hours(P0.05), but had no stimulating effect at 24 hours.EGF at concentration of 0.01 mg/L had no obvious effect on the growth of fibroblasts(P0.05).Conclusion EGF with certain range of concentration can increase the growth of corneal fibroblasts of rabbit.

AIM To study the effect of NO on dependence and tolerance of NM in the guinea pig ileum in vitro. METHODS The segment of the guinea pig ileum were suspended in organ chambers for measuring withdrawal responses are expressed as ratio of naloxone(100 nmol· L-1)and Ach (100 nmol · L-1) contraction responses and measuring inhibitory effects of NM for contractions evoked by electric stimulation. RESULTS After the segment of the ileum was incubated With NM (200 nmol· L- 1 ), NM (200 nmol· L-1) plus L-Arg (0.1 mmol· L-1), at 4℃ for 24 h respectively, the ratio are 0.89 ± 0.02, 0.90± 0.02, (n = 6) P 0.05. The ratio of NM (200 nmol· L-l) plus NOArg (0.1 mmol. L-1 ) is 0.47 ± 0.05, compared with control (n = 6, P 0 .01 ). After the segment of the ileum was incubated with NM (500 nmol· L-1 ), NM (500 nmol· L-1 ) plus L-Arg (0. 1 mmol·L-1) at 37℃ for 3 h respectively, the sensitivities of the preparations for NM were reduced significantly, their concentration- response curves were shift to right (P 0 .01 ). In the segment of the ileum incubaled with NM (500 nmol· L-１) and NG-nitro－L-Arginime (NOArg 0. 1 mmol . L- 1 ) at 37℃ for 3 h, the sensitivities of the preparations for NM was unchange (P 0 .05). CONCLUSIONS These results suggest that NO has important role on dependence and tolerance of NM in the guinea Pig ileum.