Objective To analyze the characteristics of VP1 gene sequences of Coxsackievirus A16 (CoxA16) isolated from Xi'an in Shanxi Province People's Hospital in 2011,and to identify the genotypes and investigate the virus biophysics of the CoxA16.Methods The stool samples were collected from children with hand, foot and mouth diseases (HFMD) in our hospital, and all of the stool samples were tested by RT-PCR. In order to obtain CoxA16 strains, CoxA16 positive samples were isolated by RD cell line. The nucleotide sequences of CoxA16 strain's VP1 were amplified and sequenced, then the nucleotide and amino acid sequences of CoxA16 strain's VP1 were analyzed by DNA Man, ClustalX 1.83 and MEGA 4.0. The CoxA16 virus particles were purified by ultracentrifugation, and the virus particles inactivated with formalin were analyzed by transmission electron microscopy (TEM).Results CoxA16 strain was isolated from stool samples successfully, and the genome of VP1 region had 891 nucleotides in length and encoded for 297 amino acids. VP1 gene comparison showed that the rates of homology of nucleotides and amino acids were 76.76％-97.57％ and 92.16％-100.0％, respectively, when compared with other reference strains from different regions. Phylogenetic analysis showed that CoxA16 isolates strain of Xi'an was subgenotype B1a, which was closely related to BJ10-03 strain. TEM analysis showed that CoxA16 strain were the icosahedral structure, and the diameters of CoxA16 strain were approximately 20-30 nm.Conclusions CoxA16 in Xi'an isolates strain was subgenotype B1a, and the biophysical characterization of CoxA16 virus particles were very similar to enterovirus of the piconaviridae family.
近年来,使用气雾剂进行平喘治疗,平喘效果快,无痛苦,受到广大医务人员的青睐,特别是儿科医务人员.但由于小儿易哭闹,不合作,正确操作比较困难.我科于2006年开始使用贮雾缸+气雾剂对喘息型肺炎患儿进行平喘治疗,取得了较好的效果,并对单纯使用气雾剂、气雾剂+替代物进行了对比观察,现报告如下.
目的:研究蒙脱石散剂保留灌肠与口服对比治疗小儿腹泻病的疗效.方法:以一次口服量(每日剂量分为3次)的2倍量溶于5～20mL 0.9%盐水中保留灌肠,每日1～2次作为治疗组.蒙脱石散剂口服(每日量分为3次)作为对照组.两组其余治疗相同.结果:接受蒙脱石散剂保留灌肠组的平均治疗天数明显短于口服组,且无异常副反应发生.结论:蒙脱石散剂保留灌肠对治疗小儿腹泻病疗效优于口服.
