The rabbit coat color as one of available genetic markers plays an important role in determining breed characteristics,purity,and crossing combination.The melanocortin-1 receptor gene(MC1R) is an important candidate gene for coat color heredity because some coat color changes in many species resulted from the nucletide sequence variants of MC1R gene.In this study,two primers(RMCR1 and RMCR2) were designed to amplify the 5′-flanking region sequences of MC1R from rabbit genome according to the known rabbit MC1R sequence(AM180881).A DNA fragment of 930 bp in length was cloned from 5′-flanking sequence of MC1R gene by an end tailed-PCR strategy,the homology of nucleotide was 99% with the known rabbit MC1R sequence(AM180881).

<正>家兔的毛色具有多样性,主要有黑色、蓝色、红色、喜马拉雅色、海狸色、青紫蓝色、巧克力色等20余种,家兔毛色的多样性是育种者对毛色选种选育的基础。研究表明,哺乳动物的毛发、皮肤及眼睛的颜色主要是由黑色素的数量、性质与分布决定的。哺乳动