头孢噻呋(Ceftiofur),其分子式为C19H17N5O7S3,化学名为[6R-[6α,7β(z)]]7-[[(α-氨基-4-噻唑基)(甲氧亚氨基)乙酰]氨基]-3-[[(2-呋喃羰基)硫代]甲基]-8-氧代-5-硫杂-1-氮杂双环[4,2,0]辛-2-烯-2-羧酸.该药是由Bernard labeeuw等人于1984年首次合成,其后美国法玛西亚-普强公司(Pharmacia & Upjohn)将其研制成钠盐的冻干粉及盐酸盐的混悬液,用于动物疾病的治疗.

The plasma pharmacokinetics of oxytetracycline(OTC) in 8 healthy and 8 endometritis affected cows was evaluated by using HPLC as a quantitative method.The data for the plasma concentrations of OTC were analysed using 3p97 software package.Results showed that there were no detectable concentrations in healthy cows,while the maximum concentration in cows affected by endometritis was relatively low.A one compartment open model with first order absorption provided the best fit for all the individual data based on the Akaike information criterion.The main pharmacokinetic parameters were as follow:AUC(18.53±10.92) mg·L-1·h-1,Cmax(1.66±0.84) μg/mL,tmax(0.35±0.06) h,t1/2ka(0.05±0.01) h and t1/2ke(7.67±2.44)h,respectively.Results showed that OTC was poorly absorbed with a fast absorption rate,and it was eliminated slowly from plasma after single intrauterine administration.

建立了鸡血浆中癸氧喹酯的高效液相-荧光检测法.结果表明,在0.02～10.0 μg/mL范围内,癸氧喹酯血药浓度呈线性关系.最低检测限为0.01μg/mL,回收率在84%以上,日内RSD小于6.87%.试验鸡按3 mg/kg体重分别经口单次灌服癸氧喹酯溶液剂和癸氧喹酯原料粉.给药后0.25～48 h内血药浓度均低于定量限(0.02μg/mL),无明显浓度变化曲线,无法进行药动学血药浓度-时间分析.

建立了高效液相色谱-荧光法(HPLC-FLD)来检测肉鸡肌肉、肝脏、肾脏和皮肤/脂肪组织中癸氧喹酯的残留量.将癸氧喹酯以0.05,0.2,0.6 μg/g的含量分别添加到空白组织中,测得肌肉、肝脏、肾脏和皮肤/脂肪组织的回收率大于75%,变异系数低于9%.用该方法测定肌肉、肝脏、肾脏和皮肤/脂肪组织中癸氧喹酯的最低检测限为0.01 μg/g.试验鸡以30 mg/L的癸氧喹酯的剂量连续7 d饮水给药,停药后各组织中癸氧喹酯的残留量逐渐下降;停药第3天后肌肉中检测不到癸氧喹酯,停药后第5天所有组织中均检测不到残留药物.