<正>人们自古以来就用天然除虫菊毒素控制蚊虫,而为了提高其生物活性和环境稳定性,人们通过化学结构修饰合成了许多拟除虫菊酯杀虫剂。哺乳动物体内拟除虫菊酯的主要靶标为电压门控钠离子通道。另外,电压门控钙离子通道、氯离子通道以及

Chloroperoxidase(CPO)from Caldariomyces fumago is a highly versatile oxidase.But its application is limited,because of its low accumulated concentration in the biological synthesis,poor dynamic stability,low recovery of high-purity enzyme in purification process and high price.A novel very efficient method has been developed.At first,CPO was precipitated with hydrophilic polymer(PEG 6000)in the high-saturated solution of aluminum sulfate.And then it was extracted by an aqueous two-phase system of PEG/ammonium sulfate.At last,the extracted CPO was purified by Sephadex G-75 chromatographic system.The results showed that:60% saturated(NH4)2SO4 solution could make PEG 6000 precipitate 95% chloroperoxidase.In the aqueous two-phase system CPO was prior to dissolve in bottom phase,its partition coefficient k was lower than 0.228,its yield about 65.2%,its purification was 7.4 times.Finally,the purity of CPO was slightly higher after chromatographic fractionation.The method was also provide a new method for extracting efficiently other trace biological molecules.