The study aimed to investigate the effect of anemoside B4(B4) on fatty acid metabolism in mice with colitis-associated cancer(CAC). The CAC model was established by azoxymethane(AOM)/dextran sodium sulfate(DSS) in mice. Mice were randomly divided into a normal group, a model group, and low-, medium-, and high-dose anemoside B4 groups. After the experiment, the length of the mouse colon and the size of the tumor were measured, and the pathological alterations in the mouse colon were observed using hematoxylin-eosin(HE) staining. The slices of the colon tumor were obtained for spatial metabolome analysis to analyze the distribution of fatty acid metabolism-related substances in the tumor. The mRNA levels of SREBP-1, FAS, ACCα, SCD-1, PPARα, ACOX, UCP-2, and CPT-1 were determined by real-time quantitative PCR(RT-qPCR). The results revealed that the model group showed decreased body weight(P<0.05) and colon length(P<0.001), increased number of tumors, and increased pathological score(P<0.01). Spatial metabolome analysis revealed that the content of fatty acids and their derivatives, carnitine, and phospholipid in the colon tumor was increased. RT-qPCR results indicated that fatty acid de novo synthesis and β-oxidation-related genes, such as SREBP-1, FASN, ACCα, SCD-1, ACOX, UCP-2, and CPT-1 mRNA expression levels increased considerably(P<0.05, P<0.001). After anemoside B4 administration, the colon length increased(P<0.01), and the number of tumors decreased in the high-dose anemoside B4 group(P<0.05). Additionally, spatial metabolome analysis showed that anemoside B4 could decrease the content of fatty acids and their derivatives, carnitine, and phospholipids in colon tumors. Meanwhile, anemoside B4 could also down-regulate the expression of FASN, ACCα, SCD-1, PPARα, ACOX, UCP-2, and CPT-1 in the colon(P<0.05, P<0.01, P<0.001). The findings of this study show that anemoside B4 may inhibit CAC via regulating fatty acid metabolism reprogramming.

目的 比较不同化疗药导致大鼠口腔溃疡的特点,寻找一种稳定可靠的化疗药导致的口腔溃疡模型.方法 大鼠右侧口腔黏膜内分别注射相应化疗药复制大鼠口腔溃疡模型,记录大鼠体质量、摄食量、溃疡直径;进行黏膜组织病理学观察,黏膜局部炎症因子等mRNA表达检测,脏器指数计算,外周血T淋巴细胞亚群检测.结果 造模后大鼠体质量、摄食量均明显下降;顺铂、紫杉醇组大鼠黏膜明显溃疡,组织病理学观察可见黏膜上皮受损脱落;黏膜组织IL-1βmRNA表达明显升高,EGFR mRNA表达明显降低,脏器指数无明显变化;甲氨蝶呤、长春新碱、阿霉素组无明显溃疡,但脏器指数下降,细胞因子表达下降;各组外周血T淋巴细胞亚群无明显差异.结论 大鼠口腔黏膜内局部注射化疗药顺铂或紫杉醇可形成稳定的口腔溃疡模型.溃疡直径、摄食量、细胞因子和炎症介质表达等可用于评价药物的治疗作用.

This article has sorted and analyzed the literature regarding the recent studies on Menthae Haplocalycis Herba covering its sources of varieties,harvest processing and packaging storage.Subject terms such as Menthae Haplocalycis Herba,harvesting,packaging and storage are used as a keyword to retrieve the related literature from CNKI and Wanfang Data,19 articles of which are summarized and analyzed.The quality of medicinal substances and decoction pieces of Menthae Haplocalycis Herba is closely related to its sources of varieties,harvest processing and packaging storage.The proper harvest processing and good packaging storage of Menthae Haplocalycis Herba is very significant to the improvement of the quality of medicinal substances and decoction pieces of Menthae Haplocalycis Herba and to the guarantee of clinical efficacy.

Objective:To probe the effects of Rhizoma Corydalis processed with different acids on both the content of tetrahydropalmatine and the analgesia by Rhizoma Corydalis.Method: The decoction amount of the tetra-hydropalmatine was measured by HPLC.The pain threshold was measured separately before and after mice took the drug by means of the hot plate.Result: The different processing of Rhizoma Corydalis exerted a certain influence on the decoction amount of the tetrahydropalmatine.Rhizoma Corydalis and its different acid products had an analgesic effect on the mice causing pain by hot plate.Conclusion: Of all the acid products of Rhizoma Corydalis,acetic acid ones had the least satisfactory effect.The comparison between malic acid,hydrochloric acid products and ace-tic acid product was not clearly marked.The citric acid products and the tartaric acid products had a certain advan-tage over others.

By consulting recent literature,this article summarized the advances in studies on chemical composition,pharmacologic action,processing and quality control of Pseudostellariae Radix.In recent years various kinds of compounds have been separated first time from Pseudostellariae Radix;related reports have been made about the fact that Pseudostellariae Radix can improve chronic heart failure or memory,lower blood sugar and resist oxidation,and also about the primary processing condition of Pseudostellariae Radix;the theoretical basis for us to remove fibrous roots has been proposed.The content of Pseudostellarin B,saponin,polysaccharide,heavy metal has been determined;amino acid and volatility composition have been analyzed;the research has been made on pesticide residue and fingerprint of Pseudostellariae Radix.The reference has been provided for the further research into Pseudostellariae Radix.