Objective To investigate the effect on getting rid of hepatitis B virus(HBV) in pregnancy mouse and fetal mouse infected HBV by small interfereing RNA(siRNA),and understanded the possibility of blocking vertical transmission.Methods siRNA after amplificatd inject into pregnancy mouse,with pcDNA3.1-DNA as experimental group;With nonsense RNA as control group.Blood,liver of pregnancy mouse and of fetal mouse are getted at 96h following injection.Fluorescent quantitation of HBV DNA,liver immunohistochemisty,RT-PCR of HVB mRNA were executed.Results Pregnancy mouse's blood HBV-DNA quantitation at experimental group [(2.77 ± 0.95) Lg copies/ml] was less than that at control group [(6.89 ±2.15) Lg copies/ml](P < 0.05).At pregnancy mouse of experimental group,HBsAg positive cells of liver [(22.5 ±14.6) copy]and HBcAg positive cells of liver[(28.8 ±17.3) copy]were less than those at control group[(42.5 ± 19.2) copy、(53.5 ± 23.3) copy](P < 0.05).Of fetal mouse,HBsAg positive cells of liver[(17.5 ± 9.6) copy]and HBcAg positive cells of liver[(27.5 ± 15.8) copy]were less than those at control group[(32.5 ± 15.2) copy and(45.7 ± 16.5) copy](P < 0.05).In pregnancy mouse and fetal mouse,mRNA expression of two groups were statistically significant(P < 0.05).Conclusion siRNA can reduce the expression of HBsAg and HBcAg and the replication of HBV DNA in mouse,either pregnancy mouse or fetal mouse.So it may be block up vertical transmission of HBV.

目的:母婴传播被视为乙型肝炎主要传播途径之一,本文探讨不同的分娩方式与乙肝免疫球蛋白阻断对HBV垂直传播的影响。方法:随机选择102例HBV DNA阳性母亲分娩的新生儿生后1周内的静脉血,应用荧光PCR方法检测其HBV DNA水平,观察母亲乙肝免疫球蛋白注射与两种分娩方式对新生儿感染乙型肝炎的影响。结果:阴道分娩组与剖宫产组孕妇两组乙肝二对半血清标志物和HBV DNA拷贝数差异无统计学意义(P>0.05),阴道分娩组新生儿乙肝DNA拷贝数(4.5±3.0拷贝/ml)明显高于剖宫产组(3.5±2.2拷贝/ml),差异有统计学意义(t=3.144,P<0.05)。乙肝阻断治疗后新生儿血中DNA拷贝数(3.1±1.9拷贝/ml)比未给予阻断治疗的新生儿(5.3±2.6拷贝/ml)明显减少(t=4.67,P<0.01)。结论:剖宫产分娩与母亲乙肝免疫球蛋白阻断可明显降低新生儿HBV感染率。

目的 研究8-异前列腺素F2α在早产儿脑损伤中的临床意义.方法收集2002年9月～2004年7月在我院NICU住院的46例早产儿,纠正胎为40周行NBNA评分,根据评分分为重度异常组、轻度异常组、正常组.ELISA双抗体夹心法分别检测三组间血浆、尿及脑脊液中8-I-PGF2α,并与NBNA进行相关性分析.结果三组间血、尿及脑脊液中8-I-PGF2α有明显差异(F=3.178,F=4.151,F=3.95,P均＜0.01),三组之间比较均有显著差异.8-I-PGF2α以重度异常组增高明显.NBNA评分(N/10)与尿、血及脑脊液中8-I-PGF2α相关性分析得出其与脑脊液中8-I-PGF2α呈负相关(P＜0.01,r=-0.461),而与尿、血中8-I-PGF2α无相关性.结论预后异常的早产儿早期存在血、尿与脑脊液中8-I-PGF2α的升高.早期脑脊液中8-I-PGF2α检测有可能作为早产儿神经行为表现预后早期预报的一个指标。

目的研究8-异前列腺素F2α(8-Ⅰ-PGF2α)在早产儿脑白质损伤及神经行为评价中的临床意义.方法 2002年12月-2005年3月在新生儿ICU住院的46例早产儿,纠正胎龄为40周行NBNA评分;于生后48 h使用头颅B超对早产儿颅内监测,根据脑室内出血程度分为正常组、轻度损伤组(Ⅰ级、Ⅱ级脑室内出血)及重度损伤组(Ⅲ级、Ⅳ级颅内出血).ELISA法分别检测3组间脑脊液中8-I-PGF2α.结果正常组31例,NBNA评分为(39.1±0.2)分;轻度损伤组9例,NBNA评分为(37.4±1.5)分;重度损伤组6例,NBNA评分为(31.3±3.4)分(不包括放弃治疗的2例病人).轻、重度损伤组早产儿共5例(轻度损伤组2例,重度损伤组3例),其中并伴有4例脑室周围白质软化,此5例患儿脑脊液中8-Ⅰ-PGF2α均在300 pg/mL以上.通过把8-Ⅰ-PGF2α分成<150 pg/mL、(150～250) pg/mL及>250 pg/mL三个区间,发现<150 pg/mL患儿 NBNA为(38.3±1.5)分;(150～250) pg/mL患儿NBNA为(34.9±2.4)分,>250 pg/mL患儿NBNA为(30.2±3.1)分.三区间NBNA评分比较有统计学意义(F=6.598,P<0.001).结论早产儿严重的颅内出血患儿早期有脑脊液8-I-PGF2α升高,早期监测8-I-PGF2α及脑室内出血程度可能对其神经行为预后有一定的判断作用.