OBJECTIVE:To establish an HPLC method for the determination of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.METHOD:Pheny ether chromatographic column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-0.1% phosphoric acid (containing 0.1% three ethylamine) (3:97) as the mobile phase. The UV detection wavelength was at 210 nm, with the flow rate of 1 mL x min(-1), and column temperature was at 35 degrees C.RESULT:The linearity of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin ranged between 0.078 60-3.144 microg (r = 1.000 0), 0.103 4-2.068 microg (r = 0.999 7) and 0.430 5-3.157 microg (r = 0.999 8), respectively. Their average recoveries were 98.46% (RSD 1.1%), 103.0% (RSD 1.5%) and 97.15% (RSD 2.1%), respectively.CONCLUSION:The method is simple, stable and reliable that it can be used to determine the content of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.

OBJECTIVE:To prepare the new traditional Chinese medicine preparation--pH-dependent brevisapin colon-specific tablets, and investigate its in vitro release, in order to discuss the feasibility of preparing colon-targeted traditional Chinese medicines.METHOD:With scutellarin, the active ingredient in brevisapin, as the evaluation index, coating prescriptions of the preparation was screened. The in vitro release determination method was adopted to detect the in vitro release performance of the preparation.RESULT:The in vitro release determination results showed no brevisapin in artificial pH 1. 2 dilute hydrochloric acid solution for 2 h, an accumulated dissolution rate of less than 5% in pH 6. 8 phosphate buffer solution for 4 h, but an accumulated dissolution rate exceeding 90% in pH 7. 6 phosphate buffer solution for 1 h.CONCLUSION:Brevisapin colon-specific tablets prepared can realize colon-specific release.

Bioavailability was important evaluation index of safety and effectiveness of drugs.Consult data library such as Wan Fang,CNKI,OVI and so on,comprehensive analyzed and reviewed more than 150 related data recent 10 years from domestic and abroad.Summarized the influencing factors of bioavailability from two aspects of medicine and biology,consist of the form of drugs,physicochemical property,the preparation technology,physiological environment of gastrointestinal,the delivery time and way and so on.Some new research ideas like the pro-drug strategy,the gastro-retentive drug delivery system,cyclodextrin closure technology,solid dispersion technique,co-grinding technology,nano-technology etc.for improving bioavailability of traditional chinese medicine(TCM)preparations were discussed in order to provide reference for the research on modernization of TCM preparations.

Objective:To establish a determination method of in vitro release for spray film of extract from Galla Chinensis,and investigate its in vitro release characteristics.Method:With gallic acid as index,modified Franz diffusion cell was chosen with semi-permeable membrane as in vitro permeability barrier,the content of gallic acid was determined by HPLC and in vitro release of gallic acid was calculated.Result:Affect of penetration enhancers on release effect of this spray film was not obvious,equation of in vitro release model fitted best with Kinetic equation.Conclusion:This spray film had characteristics of good release and Kinetic equation,it could be achieved a better release effect without penetration enhancers.

To review and analysis the reference literatures of researching on plastics.Refer to the relevant literatures and summarize,summarize the study progresses of plastics,including the preparation process,film-forming materials,quality control,stability,transdermal absorption and clinical application et al.The ingredients of preparation of plastics are mainly used traditional Chinese medicine,and little used Western-style pharmaceuticals.Some problems need to research,such as single in film-forming materials and inadequate in quality standards.Plastics preparation is simply and easily to use.Therefore,if develop the reasonable quality evaluation methodology,plastics will have wider medical applications.

Objective:To optimize extraction technology of Salvia miltiorrhiza.Method:With the content of salvianolic acid B,total salvianolic acid,tanshinoneⅡA,total tanshinone as indexes,the content of index ingredients were determined by HPLC,UV spectrophotometry and colorimetry,ethanol refluxing extraction technology was optimized by orthogonal test with taking the concentration of ethanol,extraction time,the amount of ethanol,size of material and extraction times as factors.Result:Optimum extraction technology was:S.miltiorrhiza was cut into sections,extracted 3 times with 10 folds the amount of 60% ethanol,2 hours each time.Conclusion:Extraction rate of water-soluble and fat-soluble constituents from S.miltiorrhiza was high by optimized technology,and it was stable and energy-saving.