Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro. Treatment of cultured CRC HCT-8 cells with CEACAM-1-specific siRNA successfully downregulated CEACAM-1 expression by 61% compared with control cells. The effects of CEACAM-1 downregulation on HCT-8 cell proliferation and apoptosis were then assessed via Cell Counting kit-8 assay and flow cytometry, respectively. The results demonstrated that siRNA-induced CEACAM-1 downregulation significantly inhibited proliferation and increased apoptosis, but had no significant effect on cell cycle progression in HCT-8 cells. Together, these results suggest that CEACAM-1 activity is critical to CRC growth, and thus, CEACAM-1 may be a promising therapeutic target for the treatment of CRC.

Objective: This study was aimed to investigate the relationship between the expression of drebrin (DBN1) gene and resistance in colon cancer to reveal the mechanism of tumor drug resistance and provide a basis for the reversal of this drug resistance in tumor cells. Materials and Methods: The human colon carcinoma cell line HCT-8 was used, and vincristine (VCR)-resistant colon cancer cell line HCT-8/V was established by gradually increasing the concentration of VCR. Polymerase chain reaction (PCR) primers were designed for DBN1 gene. The DBN1 differential expression in colon cancer sensitive and resistant cell lines was detected by fluorescence quantitative PCR. Western blot analysis was used to study DBN1 expression in the resistant cells further. Results: VCR resistance of HCT-8/V cell line was established. Quantitative PCR and Western blot results showed that DBN1 expression in the resistant cell line was significantly lower, the difference being statistically significant (P < 0.05). Conclusion: Low DBN1 gene expression may be associated with colon cancer cell resistance to VCR.

Background Tuberculosis (TB) is a major public health concern worldwide, and spreads more easily in densely populated areas such as school campuses. Objectives The aim of this study was to determine the prevalence of positive TB skin tests among freshmen, i.e. newly-enrolled college students, in Zhengzhou City, China. Methods We reviewed the data of purified protein derivative (PPD) skin tests in 656,212 freshmen in 2004–2013. Results A positive test showed a diameter of swelling ≥ 5 mm. The PPD positive rate was 40.69 %, with a prevalence of 146.29 per 100,000. During the 10-year study period, the rate of students with positive PPD test increased from 34.19 % in 2004 to 40.69 % in 2013. The positive PPD rate was significantly higher in males than in females (41.68 % vs 39.61 %, P<0.0001), and in rural compared with urban areas (42.04 % vs 38.03 %, P<0.0001). Conclusion These findings indicated a high prevalence of PPD positivity among participants during the study period, with an increasing trend. Therefore, this population needs to be targeted by TB prevention and control programs.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的了解河南省农村老年人生活质量及其影响因素,为相关行业及政府部门完善相关政策提供依据。方法采用多阶段整群随机抽样法抽取河南省6个地市60个自然村共4 850名农村老年人,采用自编健康状况问卷和SF-36健康调查简表进行问卷调查。结果 4 850名老年人生活质量8个维度得分从高到低依次为情感职能（70.20±25.81）分,活力（66.33±14.85）分,生理功能（63.46±22.45）分,生理职能（63.15±26.11）分,精神健康（61.2±11.1）分,一般健康状况（50.10±11.64）分,社会功能（44.60±13.00）分和躯体疼痛（36.06±22.04）分;多因素logistic回归分析结果表明,河南农村老年人生活质量的主要影响因素为年龄、婚姻状况、配偶情况、子女数、子女孝敬程度、是否患慢性病、居住方式（P&lt;0.05）。结论河南农村老年人生活质量较差,生活质量影响因素具有多维性;子女的关心、照顾和家庭的温暖,完善农村医疗保健制度和提高医疗水平,加大社会支持可提高农村老人的生活质量。</span>

OBJECTIVE To understand the prevalence of Pseudomonas aeruginosa infection and investigate the change of drug resistance from 2011 to 2013 so as to provide scientific basis for reasonable clinical use of antibiot‐ics .METHODS From Jan 2011 to Dec 2013 ,totally 1 053 strains of P .aeruginosa were isolated from the clinical specimens ,the isolation ,culture ,and identification were carried out according to the national clinical laboratory operation rules ,the drug susceptibility testing was performed with the use of MIC method ,and the data were sta‐tistically analyzed by using SPSS 13 .0 software .RESULTS Of 1053 strains of P .aeruginosa isolated from 2011 to 2013 ,991 (94 .11% ) strains were isolated from the sputum specimens .The drug resistance rates to sulfamethox‐azole‐trimethoprim ,chloramphenicol ,and minocycline were 100 .00% in the three years ;the drug resistance rates to aztreonam ,piperacillin‐tazobactam ,cefepime ,cefoperazone‐sulbactam ,polymyxin B ,and colostin were less than 20 .00% in the three years ;the drug resistance rate to imipenem increased from 6 .65% in 2011 to 37 .24% in 2013 ,and the drug resistance rate to meropenem increased from 8 .82% in 2011 to 21 .62% in 2013 . CONCLUSION It is necessary to strengthen the surveillance of drug resistance of the P .aeruginosa strains and rea‐sonably use antibiotics so as to control the emergence of the drug‐resistant strains .

目的 了解2011-2013年临床分离鲍曼不动杆菌(ABA)的耐药性变迁,为临床治疗和预防提供依据.方法 按《全国临床检验操作规程》(第3版)分离鉴定鲍曼不动杆菌,药敏试验采用MIC法.结果 2011-2013年共检出鲍曼不动杆菌726株,从痰液中分离682株,占93.94％;3年耐药率最高的药物是诺氟沙星,为100％;3年耐药率均＜20.00％的抗菌药物为米诺环素和多黏菌素B;耐药性增长幅度较大的药物是氨苄西林/舒巴坦、头孢哌酮/舒巴坦、亚胺培南和美罗培南.结论 加强鲍曼不动杆菌耐药性监测,合理使用抗菌药物,是预防和控制鲍曼不动杆菌感染的有效措施.

目的 了解医院下呼吸道感染患者痰标本病原菌分布和耐药性,为临床合理用药提供依据.方法 对2011-2014年医院下呼吸道感染患者痰标本中病原菌耐药性进行回顾性研究.采用珠海迪尔医学细菌测定系统进行细菌鉴定和药敏试验.结果 12 200份痰标本中分离病原菌4 559株,检出率为37.37％.其中革兰阴性杆菌93.57％,排列前四位的细菌是铜绿假单胞菌菌(27.79％)、鲍曼不动杆菌(17.77％)、肺炎克雷伯菌(17.70％)和大肠埃希菌(6.89％);革兰阳性球菌占3.99％,以金黄色葡萄球菌为主(1.21％);真菌占2.44％,以白假丝酵母菌最多(1.69％).药敏结果显示,下呼吸道感染病原菌对大部分抗生素有不同程度耐药且耐药特点各不相同.4年中铜绿假单胞菌对哌拉西林/他唑巴坦、头孢哌酮/舒巴坦、头孢吡肟、美罗培南、阿米卡星和多黏菌素B耐药率始终低于30％,其平均耐药率分别为11.60％、5.84％、11.05％、16.50％、12.00％和5.13％;鲍曼不动杆菌对米诺环素和多黏菌素B较为敏感,耐药率分别为7.16％、2.22％;肺炎克雷伯菌对亚胺培南和美罗培南的耐药率为4.21％;大肠埃希菌对亚胺培南的耐药率为0.00％.未检出耐万古霉素、利奈唑胺和替考拉宁的金黄色葡萄球菌以及耐氟胞嘧啶和两性霉素B的白假丝酵母菌.结论 下呼吸道感染病原菌耐药情况较为严重,应规范抗生素的使用,控制细菌耐药性变异的发生.

目的:了解河南农村老年人生活质量、幸福感及其影响因素.方法:采用多阶段随机抽样法对河南省六个地市60个自然村共4651名≥60周岁老年人进行问卷调查.结果:河南农村老年人生活质量较差,空巢老年人感到幸福的为77.5％,与子女同住老年人感到幸福的为87.8％,差异有统计学意义(P＜0.05).性别、年龄、文化程度、婚姻及配偶情况、有无子女及子女孝敬程度、患慢性病情况及医药费用、经济状况、居住方式与老年人幸福感有关(P＜0.05).结论:河南省农村与子女同住老年人比空巢老年人感到幸福,社会、家庭和子女要加强对空巢老年人的关心和照顾.

目的：了解河南省农村不同居住方式老年人身心健康状况及其影响因素。方法采用多阶段随机抽样法对河南省六个地市60个自然村共4850名≥60周岁老年人进行问卷调查。结果农村单独居住养老、与子女同住养老和机构养老三种不同居住方式老年人的身心健康状况得分比较，其生理职能（RP）、躯体疼痛（BP）、总体健康（GH）、活力（VT）、社会功能（SF）、情感职能（RE）、精神健康（MH）得分差异均有统计学意义（P＝0．000），但生理功能（PF）得分差异无统计学意义（P＞0．05）。结论河南省农村不同居住方式养老的老年人身心健康状况不同，性别、年龄、文化程度、职业、婚姻状况、住房面积、子女数和患病种数是河南省农村老年人生存质量的影响因素。

The present study aimed to investigate the effects of human brain-derived neurotrophic factor (hBDNF) on the differentiation of bone marrow mesenchymal stem cells (MSCs) into neuron-like cells. Lentiviral vectors carrying the hBDNF gene were used to modify the bone marrow stromal cells (BMSCs) of Sprague-Dawley (SD) rats. The rat BMSCs were isolated, cultured and identified. A lentivirus bearing hBDNF and enhanced green fluorescent protein (eGFP) genes was subcultured and used to infect the SD rat BMSCs. The expression of eGFP was observed under a fluorescence microscope to determine the infection rate and growth of the transfected cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was used to detect the proliferation rate of cells following transfection. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot analysis were used to detect the expression levels of hBDNF. Differentiation of neuron-like cells was induced in vitro and the differentiation rate of the induced neural-like cells was compared with that in control groups and analyzed statistically. In the cultured cells, flow cytometry demonstrated positive expression of cluster of differentiation (CD)90 and CD44, and negative expression of CD34 and CD45. The proliferation rate of the rat BMSCs increased following gene transfection. The expression of hBDNF-eGFP was detected in the BMSCs of the experimental group. The differentiation rate of hBDNF-modified cells into neuron-like cells in the experimental group was higher compared with that in empty plasmid and untransfected negative control groups. The difference was statistically significant (P<0.05). Thus, BDNF gene transfection is able to promote the differentiation of BMSCs into neuron-like cells. BDNF may play an important role in the differentiation of MSCs into neuron-like cells.

The pathogenic bacterial distributions and resistance states in patients with lower respiratory infections were analyzed to provide evidence for clinical therapy. Bacteria were cultured and separated according to clinical routine. A bacteria measuring medical software was used for statistical analysis. A drug sensitivity test was performed using the minimal inhibitory concentration (MIC) method. The detection rate of pathogenic bacteria in phlegm samples was 38.61%. Gram-negative bacilli comprised 94.31% of the total bacterial population. The four common bacteria were Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae, and Escherichia colt. The drug sensitivity test showed that the pathogenic bacteria, which can cause pulmonary infections, had different degrees of resistance to many antibiotics. Only cefoperazone/sulbactam achieved better sterilizing effects on the four common Gram-negative bacilli than the other antibiotics. The resistance rates were below 10%. Pathogenic bacterial resistance was poor in upper respiratory infections. Thus, antibiotic use should be standardized to control the variation in bacterial drug resistance.

目的了解高校新生结核菌感染情况,为高等院校防治结核病提供科学依据。方法对郑州铁路职业技术学院连续7届全部入校新生采用PPD试验进行检测,对强阳性者进行胸透、摄片及痰涂片检查;在2011—2012届学生中随机抽样进行卡痕率检测;对学生结核病防治知识进行现场问卷调查。结果 7届学生PPD试验阳性率呈上升趋势(χ2=143.49,P<0.05);男生强阳性率及患病率均高于女生(χ2值分别为16.84,12.55,P值均<0.05);农村学生强阳性率及患病率均高于城市学生(χ2值分别为160.43,5.11,P值均<0.05);外省学生强阳性率及患病率均高于河南省学生(χ2值分别为10.53,6.39,P值均<0.05);与患者有密切接触史的学生强阳性率高于同年级学生(χ2=29.96,P<0.05);城市学生的卡痕率明显高于农村学生(χ2=118.27,P<0.05);农村学生对结核病的防治知识知晓率较低(42.03%)。结论高校学生的结核疫情不容忽视。应采取有效措施,防止结核病在高校暴发流行。

目的探求风疹与麻疹的鉴别依据。方法对经血清学证实的风疹和麻疹病人的临床资料进行回顾性分析。结果一般发热、呼吸道卡他症状、眼结膜炎症状、皮疹等麻疹相对较重;Koplik斑、出疹时间、耳后及枕部淋巴结肿大是重要的鉴别依据。结论在病程的1~2 d出疹无Koplik斑者为风疹的可能性最大。

目的 探讨外周血脑钠肽(BNP)和一氧化氮(NO)检测对高血压病并发急性冠状动脉综合征(ACS)的临床意义.方法 选取健康受试者40例、单纯高血压患者40例、未合并高血压ACS组30例、高血压合并ACS组31例,分别测定血浆BNP和NO水平.结果 高血压组与对照组相比血浆BNP水平明显升高,NO水平明显降低(P<0.05);ACS组、高血压并发ACS组BNP进一步升高,NO进一步降低,与对照组有极显著差异(P<0.01),与高血压组有显著差异(P<0.05).结论 高血压病并发或不并发ACS患者均有炎症活动,检测血浆BNP和NO水平对高血压病患者并发ACS有重要的预测价值.

Objective To observe the effect of resveratrol(Res)on proliferation of esophageal cancer(Eca-109)cells,and explore its mechanism.Methods Eca-109 cells were cultured with different concentrations of Res(0,10,20,40 μmol/L)and different times(24,48,72h).The cells proliferation was detected by MTT method and the expression of the VEGF gene was detected by RT-PCR.Results The inhibited effect of Res was enhanced as the increasing of Res concentration and time.The expression of VEGF gene was also repressed by Res.Conclusion Res can significantly inhibit the proliferation of Eca-109 cells and it may be relative with the repression of the VEGF gene expression.

目的 探讨尿微量蛋白如α1-微球蛋白(α1-MG)、β2-微球蛋白(β2-MG)、微量白蛋白(mAlb)、免疫球蛋白G(IgG)和N-乙酰-β-D-氨基葡萄苷酶(NAG)对慢性阻塞性肺病患者肾脏损害的早期诊断价值.方法 以60例健康青壮年和健康老年人为正常对照组,95例慢性阻塞性肺病(COPD)根据是否合并呼吸衰竭分为2组.所有受试者留晨尿10ml离心,收集上清液,检测尿四项微量蛋白和NAG.结果 老年组尿α1-MG、β2-MG与健康青壮年对照组有差异(P<0.05),其余各项指标无差异(P>0.05);单纯COPD组患者,尿α1-MG、β2-MG、mAlb与健康青壮年对照组和老年组有显著性差异(P<0.01),尿NAG和IgG尿和IgG与上述两组无显著性差异(P>0.05);COPD合并呼吸衰竭组患者,尿NAG和α1-MG、β2-MG、mAlb、IgG与其余三组组比较均有显著性差异(P<0.01).结论 尿微量蛋白和NAG可作为慢性阻塞性肺病肾脏损害的早期诊断指标.

[Objective] To investigate the influence of the polluted black river on children's growth and development,and provide a scientific gist for assessment the polluted status of the black river.[Methods] A total of 3427 random samples aged 5-12 selected from 5 town in drainage area were divided into two groups.We analyzed the following index:the body mass indexes(height,weight,the bust),liver indexes(size,function,HBV infection status)and nutrition indexes(erythrocyte count,hemoglobin,trace element).[Results] Compared with controls,the body mass indexes of the experimental group were lower(P﹤0.05)and the sizes of liver were higher(P﹤0.01).Both of the two groups subjected to severe anemia and the experimental group had a higher rate of anemia(P﹤0.01).There were no significant difference in trace element,liver function indexes and HBV infection status.[Conclusion] This study indicated the pollution has been obviously harmful to children's health in the black river area.

目的 了解营养知识宣教对改善学生营养性贫血的作用.方法 在血红蛋白9～11g/dl范围内、7～12岁小学生随机抽取90名分为实验组(45例)、对照组(45例),对实验组学生家长进行营养知识宣教而对照组家长不宣教.30d后观察两组学生饮食改善情况及血色素指标改善情况;血红蛋白测定采用氰化高铁法.结果 实验后胃纳情况、贫血症状、挑食、偏食现象、血色素指标实验组与对照组均比以前有所好转或提高,而实验组改善更为明显,两组相比差别有显著性.结论 加强营养知识宣教,增强学生及家长合理膳食观念是改善学生营养性贫血的关键.

冠心病(CHD)是冠状动脉粥样硬化所引起的一种心血管疾病,临床上较为常见,而且发病率和病死率均很高,有研究显示低浓度血清胆红素(Bil)是CHD的独立危险因素,血清尿酸(UA)水平升高与CHD成正相关[1-2].为了进一步研究Bil、UA水平与CHD的关系,作者检测了不同病变程度冠状动脉Bil和UA水平,并进行对比分析,报告如下: