目的 对熏倒牛药材小鼠灌胃的急性毒性、家兔皮肤毒性进行实验研究.方法 采用寇氏法原则设计实验,将熏倒牛药材的提取物小鼠灌胃后观察其急性毒性,并按规定计算半数致死量(LD50)和95％可信限.将熏倒牛药材的提取物涂抹家兔完整皮肤、破损皮肤,观察单次给药与多次给药的皮肤毒性.结果 熏倒牛药材提取物对小鼠灌胃的LD50为34.0 g/kg,95％可信限为30.83～37.5 g/kg;熏倒牛药材提取物对家兔完整皮肤与破损皮肤均无明显的毒性.结论 对熏倒牛药材的毒性研究可为临床用药的安全性、资源的开发利用提供参考.

Objective To establish the quality standard of Biebersteinia heterostemon medicinal materials.Methods The morphological identification and microscopic identification were conducted for medicinal materials.The content of luteolin in Biebersteinia heterostemon was determined by HPLC.Mobile phase was methanol-0.4% phosphoric acid solution(48∶52),and detection wavelength was 350 nm.Results There were many catheters,glandular hairs and nonglandular hairs in microscopic structure of medicinal materials.The fiber bundle was scattered or binded.Pollen grains was existed.Stoma structure was visible in leaf epidermis cells.The average water content of medicinal materials was 9.80%,and the average content of luteolin was 0.745 mg/g.Conclusion The content determination method established in this experiment can be used for quality control of Biebersteinia heterostemon medicinal materials.

Integrative medicine treatment on bacterial infection in 1 case shows that the misuse of antibiotics is guided selection of microbial,which lead to poor efficacy.Although there was no super bacteria concept in TCM,butits diagnosis and treatment complied with the law of typhoid fever and febrile disease.In TCM the treatment time is short,effective,in expensive and quick,simple prescription,which all reflect the uniqueness of TCM on treatment of bacterial infection.