近年来,肠道微生物与畜禽生产性状相关性研究成为动物养殖领域的研究热点.本研究探讨了不同性别河流型水牛的肠道微生物种群结构的异同,研究结果表明,河流型水牛肠道微生物主要以Firmicutes、Eur-yarchaeota、Spirochaetes和Proteobacteria为主要菌门,且不同性别的水牛肠道微生物在门水平的种群结构相似,但是各菌门在两组样本中的分布存在一定差异,公水牛样本中Verrucomicrobia丰度显著高于母水牛组的.在属水平,母水牛组样本中Peptostreptococcaceae noname和Lysinibacillus的丰度显著高于公水牛组的,而Streptococcus和Klebsiella的丰度显著低于公水牛组的.两组样品在种水平上母水牛组与公水牛组中Pepto-streptococcaceae noname unclassified、Lysinibacillus unclassified、Methanobrevibacter smithii、Streptococcus infan-tarius和Klebsiella pneumoniae等5种物种的相对丰度存在显著差异(P＜0.05).本研究对不同性别河流型水牛的肠道微生物的种群结构进行初步的研究,为研究水牛肠道菌群结构与生产性状的相关性奠定了工作基础.

上世纪80年代以来，我国房地产业取得了突飞猛进的发展，企业数量日益增多，企业规模不断扩大，逐步成为我国国民经济中的一个重要产业，尤其是从1998年开始，房地产业表现出非常迅速的上升态势。随着房地产的不断升温，国家出台了一系列的调控措施，而在对房地产的调控政策当中，房产税一直是个争议性的话题。上海与重庆两市已于2011年1月28日正式开启了房产税改革的试点工作，2013年以来，国务院、国家发改委、财政部、国家税务总局等相关机构都先后表态，要逐步扩大房产税改革试点范围。但房产税的推广并非易事，房产税的全面征收牵涉面太广，在公民权利日益觉醒的情况下，如何提高房产税纳税人的纳税遵从度，是一个我们必须去思考的新问题。

<正>随着国家在保障性住房方面推进力度的不断加大,全国各地都在保障房建设方面展开了积极的探索。其中,2011年底武汉市洪山区南湖村、马湖村公租房建设的"洪山模式"引起了大家的广泛关注。一、武汉公租房"洪山模式"初期遇冷2011年,中央确定了全国要开工建设保障性住房1000万套的目标,武汉市政府的建设任

<正>随着房地产的不断升温,国家出台了一系列调控措施,从2004年的"831大限"提高拿地门槛开始,其后不断加码。自2009年12月份以来,调控政策越来越严厉,短短3年时间经历了五次升级,分别是2010年1月的"国十一条"、4月的"国十条"、9月的"9.29新政",2011年1月的"新国八条",以及2013年2月20日出台的"国五条"。2013年5月19日,又有媒体报道,房产税试点扩容已提上日程,今年有望新增数个城

自从1997年亚洲金融危机及2008年次贷危机以来,兴起了对巩固经济和金融风险管理的研究热潮。本文针对我国的金融风险,结合我国的实际情况,提出了我国宏观调控政策今后的走向。

自从1997年亚洲金融危机尤其是2007年次贷危机以来,学术界兴起了对巩固经济和金融风险管理的研究热潮。本文首先分析了金融危机的外部冲击的主要形式——投机冲击,然后构建了金融危机传导的时间层面和空间层面框架,最后系统地阐述了金融危机传导的具体途径。

In recent years,logistics finance has been a hot topic in logistics industry day by day as a new operation.Logistics finance can bring not only considerable benefit but also many risks bucause it is not mature sufficiently.This article analyzes the flaw of fundamental mode and the potential risks of logistics finance and puts forward the proposal of keep away risks from logistics finance.

随着计算机能力的日益强大、数据挖掘和数据分析工具能力的显著增强、电子商务的兴起以及通过互联网进行客户关系开拓能力的发展等,客户关系管理的发展非常迅速。如今客户的渠道选择越来越宽,客户关系管理成功的核心就在于需要在多渠道环境中更加有效地管理客户关系,并在这种渠道环境中产生大量良好的客户体验。

Multiple Equilibrium is one of the important theories of financial crisis. In this article, we investigate the mechanism of multiple equilibrium area of financial crisis. Based on this, we discuss the game theory with public, government and speculator, and then confirm the importance of government in the multiple equilibrium area of financial crisis. The conclusion we draw may help government to deal with financial crisis when in multiple equilibrium area.

In a world of globally integrated financial markets and high capital mobility, how to collect effective taxation of cross-border income has become problematic under international tax practices. A feasible method to solve the puzzle is to collect withholding taxes at source. This paper compares Chinese withholding taxation with other countries in the world from the aspects of tax base, taxpayers and tax rates. With such comparison analysis, it provides some helpful suggestions to improve Chinese withholding tax system.

Objective To investigate the effect of TNF-α on the expression of Notch1 in Raji cell line. Methods The expression level of Notch1 mRNA and protein in Raji cells treated with TNF-α was detected by RT-PCR and flow cytometry respectively. Results The expression of Notch1 mRNA was up-regulated in a dose-dependent manner in Raji cells treated by TNF-α (P<0.01). Raji cells treated with TNF-a had a higher fluorescence intensity of Notch1 protein as compared with untreated cells (P<0.01). Conclusion Notch1 protein was expressed in Raji cells and the expression of Notch1 protein was increased after TNF-α treatment, suggesting that activated Notch1 signaling pathway plays an important role in pathogenesis of lymphoma and it might be a potential new target for treatment.

<正>我国房地产企业涉及到的主要税种有:营业税、土地增值税、企业所得税、房产税、契税等。综合分析房地产企业应缴纳的各项税收及其政策规定,其计税依据具有一定的重复性。如房屋出租的营业税、房产税等都以租金收入为计税依据;销售不动产的营业税、土地增值税,以房地产销售价格计税或与此有关

OBJECTIVE To investigate the effect of curcumin and tumour necrosis factor(TNF)-α on the expression of vascular endothelial growth factor in Raji cells and their effect on angiogenesis in human umbilical vein endothelial cells(HUVECs)-derived cell line(ECV304).METHODS VEGF secreted by Raji cell line was determined by ELISA.Angiogenesis was tested by network formation of endothelial cells on Matrigel.Levels of VEGF mRNA in Raji cells and Notch1 mRNA in ECV304 cells were measured by RT-PCR.RESULTS The content of VEGF secreted by Raji cells was increased by TNF-α and suppressed by curcumin(P0.01).The VEGF mRNA expression,mainly VEGF165 and VEGF121 in Raji cells(containing 165 and 121 amino acid residues,respectively) was detected in all fractions.TNF-α augmented the expression of VEGF165 and VEGF121 mRNA and curcumin reduced the expression(P0.01).No network and cord were formed in control and curcumin group.There was tube formation on matrigel in the supernatants of Raji culture group and the supernatants groups treated by VEGF and TNF-α in Raji cell.Notch1 mRNA was detected in ECV304 cell line but no difference in VEGF group compared with control(P0.05).CONCLUSION The expression of VEGF in Raji cells was increased by TNF-α and suppressed by curcumin.VEGF and TNF-α can induce angiogenesis and curcumin can inhibit angiogenesis in ECV304 cells.

AIM: To investigate the effect of triptolide on the expression of vascular endothelial growth factor (VEGF) in Raji cell line and on angiogenesis in cell line (ECV304). METHODS: The experiment was performed at the Molecular Biology Laboratory of Public Health Constitute, Tongji Medical College, Huazhong University of Science and Technology from August 2005 to February 2006. ①The antiproliferative effects of triptolide (3.125, 6.25, 12.5, 25 and 50 nmol/L) against Raji cells were determined by the MTT method at 12, 24, 36, 48, 60 and 72 hours. ②After 24 hours incubations treated by different concentrations of triptolide (12.5, 25, 50 nmol/L), the mRNA level of VEGF by Raji cells was measured by reverse transcription-polymerase chain reaction (RT-PCR). ③After 24 hours treatment with 25 nmol/L triptolide, VEGF concentrations in cell culture supernatants were determined by enzyme-labeled immunosorbent assay (ELISA). ④ Effect of triptolide on angiopoiesis of ECV304 cells in matrigel was detected by angiopoiesis test. RESULTS: ①The triptolide significantly inhibited the proliferation of Raji cells in time- and dose-dependent manner. IC50 value was 25 mmol/L. ②The mRNA expression of VEGF165 and VEGF121 (containing 165 and 121 amino acid residues, respectively) was detected in all groups. Triptolide reduced the mRNA expression of VEGF165 and VEGF121 in a dose-dependent manner. ③ Triptolide significantly suppressed VEGF expression in Raji cells (P < 0.01). ④There was blood vessel formation on matrigel in the supernatants from Raji culture group and VEGF group. No network and cord formed were seen in control and triptolide groups. CONCLUSION: Triptolide could inhibit the proliferation of Raji cells in time- and dose-dependent manners as well as the expressions of VEGF in Raji cells and angiogenesis in ECV304 cells.

The financial crisis in Asia, beginning with pressures on the Thai baht and spreading to other countries in the region, has intensified the interest in impulse-contagion mechanism of financial crisis. In this article, first we discuss speculative attack, which is the main form of external impact to financial crisis. Then we construct the time and spatial contagion frame of financial crisis. Finally we analyze the general contagion mechanism of financial crisis systematically.

OBJECTIVE To investigate the effect of trichostatin A,one kind of HDAC inhibitors,on expression of IκB-α induced by TNF-α and activation of NF-κB in U937 cells.METHODS U937 cells were treated with TSA,TNF-α respectively or with both,the protein expression and subcellular localization of NF-κB/p65 were observed by western blot and fluorescence microscopy respectively;the expression and degradation of IκB-α were investigated through by flow cytometry and western blot.RESULTS ①Under fluorescence microscopy,NF-κB/p65 was only localized in cytoplasm of empty control and group-treated with TSA for 45 min,p65 was localized in nucleus of U937 cells treated with TSA and TNF-α,while it was expressed in both cytoplasm and nucleus of cells treated with TNF-α.Western blot also showed the same result:TNF-α-treated group cells nuclears expressed much more p65 protein than those treated with TNF-α plus TSA for 45 min.②After being treated with TSA and TNF-α for 45 min,the protein expression of IκBα in U937 cells detected by flow cytometry was stronger than those only treated with TNF-α(P<0.05).Through Western blot,it was found that IκB-α expression was fewer in TNF-α-treated group compared to control;which was obviously enhanced in group treated with TSA plus TNF-α than that treated with TNF-α for 45 min,but it was no differences between them after being treated for 2 h.CONCLUSION TSA can partially inhibit degradation of IκB-α induced by TNF-α and NF-κB activation in U937 cells.TSA can work through NFκB signal pathway against tumor,which can be a target of TSA for cancer therapy.

文章介绍了第一代货币危机模型和第二代货币危机模型的基本观点,在此基础上讨论了亚洲货币危机中货币危机的蔓延机理,并对我国货币危机的防范提出了几点建议。

The study was purposed to investigate the effect of deguelin on expression of nucleoporin 98 (nup98) in leukemia K562 cells. MTT assay was used to assess the effects of deguelin on cell proliferation. FCM and RT-PCR were used to analyze the changes of nup98 mRNA and protein in K562 cells after treating with deguelin. The results showed that deguelin inhibited the proliferation of K562 cells in a time- and dose-dependent manner; mean fluorescence intensity of nup98 in blank group (34.22 +/- 1.63) was significantly higher than that in control group (2.83 +/- 0.02, P < 0.01), 10 nmol/L deguelin could significantly inhibit the expression of nup98 protein; 10 nmol/L could not inhibit the expression of nup98 mRNA, 20, 40, 80, 160 nmol/L deguelin could significantly inhibit the expression of nup98 mRNA in a dose-dependant manner. It is concluded that deguelin inhibits the proliferation of K562 cell through inhibiting the expression of nup98, and may be considered as a new target for therapy of acute leukemia.

目的:探讨鱼藤素对DNA拓扑异构酶Topo Ⅰ表达的影响,进一步明确鱼藤素抑制肿瘤细胞增殖的作用机制.方法:流式细胞术及RT-PCR法分析鱼藤素作用K562细胞后Topo Ⅰ蛋白及mRNA含量的变化.结果:流式细胞术分析结果发现Topo Ⅰ蛋白的平均荧光强度随着鱼藤素作用浓度的增高而逐渐减少,与空白对照组比较有显著差异(P＜0.05);RT-PCR半定量检测结果显示,鱼藤素作用K562细胞引起Topo Ⅰ mRNA含量减少,并与其浓度正相关.结论:鱼藤素可通过抑制肿瘤细胞内Topo Ⅰ而发挥作用.

Early warning systems help detect financial vulnerabilities and prevent financial crises. This paper establishes a new Early Warning System(EWS)for predicting financial crises based on P-S method and conducts empirical researches with the data of China and those countries that broke out financial crisis in 1997 like Thailand, Korea, Indonesia, Malaysia from 1992 to 1996.