Objective:To explore the mechanism of interferance of iron ion with the antifungal drug-Ciclopirox olamine. Methods:To utilize micro broth dilution method and disk diffusion method to determine whether the iron levels is critical factor for the antifungal action of Ciclopirox against Microsporum canis.Results:Iron ion depressed the antifungal action of Ciclopirox against Microsporum cani.Conclusion:Iron ion can join in the antifungal action of Ciclopirox against Microsporum cani.

头癣是儿童常见的浅部真菌病.我们调查了引起儿童头癣的病原真菌谱及病原真菌对常用抗真菌药物的敏感性.

Objective Study on the broth microdilution antifungal susceptibility testing condition about 182 Microsporum canis derived from patients in Beijing Children's Hospital.Methods The vitro method was used for testing filamentous fungi.We approached the broth microdilution antifungal susceptibility testing condition for Microsporum canis and tested the minimal inhibitory concentration(MIC) of 8 common antifungal drugs.Results The broth microdilution antifungal susceptibility testing condition for Microsporum canis: inoculum size ranged from 1×10~4 to 3×10~4 CFU/mL;MICs obtained from different medium at the same incubation time for the same isolate were significantly different,MICs were consistently higher with SDB than with RPMI-1640,when itraconazole,bifonazole,amorolfine were tested,no parameter had any influence on MICs;incubation temperature was 28 ℃,Microsporum canis produced clearly detectable growth after 5 d.Conclusion With good reproducibility and feasibility,the M38A procedure can be used for the antifungal susceptibility test to dermaphytes in the laboratories.

A 15-year-old female manifested cutaneous lesions on her face and hand for 10 years. Septate hyphae were observed microscopically in scrapings from the surfaces of the eruptions. Fusarium moniliforme was isolated from two skin biopsy specimens. Histological examination revealed the presence of numerous septate hyphae in the dermis. Fusarium infection was confirmed by cultures from the specimens. Combined therapy of surgery and oral terbinafine led to clinical and mycological cure.

为了解北京及其周边地区的头癣流行状况及病原菌分布,对508例头癣患者病发均做了真菌直接镜检和真菌培养.结果直接镜检阳性423例(83.3%),培养阳性357例(70.3%).分离出犬小孢子菌263株(73.7%),须癣毛癣菌72株(20.1%),红色毛癣菌8株(2.2%),石膏样小孢子菌7株(2.0%),紫色毛癣菌6株(1.7%),铁锈色小孢子菌1株(0.3%).结果示头癣是儿童常见的真菌感染性皮肤病,在北京地区主要致病菌为犬小孢子菌和须癣毛癣菌,未发现黄癣菌.

对52名脱发患儿头发中微量元素铜、铁、锌、钙、镁、铅含量进行测定,并与60名正常对照组健康儿童作对照.研究分析结果显示,脱发患儿头发中铜、锌的含量低于正常对照组,并具有统计学意义(P＜0.01),而其它几种元素,脱发患儿与对照组无明显差异(P＞0.05).提示:脱发儿童应及时进补微量元素铜、锌.