Seed dormancy is an adaptive trait that widely exists in angiosperms and gymnosperms. The mechanisms for the release of seed dormancy have been less well studied. Using smoke tree (Cotinus coggygria var. Cinerea Engler) seeds, the effect of cold moist stratification (5 °C, 18.5 % humidity and 0–75 days) on dormancy release, changes of respiration rate, ABA and GA3 content, and the differentially expressed proteins during dormancy release were investigated. Seed dormancy was released during cold moist stratification, seed respiration rate was increased while both ABA and GA3 concentrations were decreased. A total of 28 protein spots with significant changes in relative expression abundance were detected by two-dimensional electrophoresis. Among these protein spots, four proteins of ATPase β subunit, heat-shock cognate protein 70, aspartic proteinase 1 and actin were successfully identified by the matrix assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. These four proteins were all down-expressed during dormancy release, and their possible implications for dormancy release of C. coggygria seeds was discussed. A better understanding of seed dormancy release and germination has practical benefits to seedling production for forest regeneration purposes.

Genetic diversity of Dipsacus asper of eight different areas in Enshi by using RAPD molecular markers.5 among 50 primers were selected to amplify about 53 segments DNA.Cluster analysis using NTSYS software,a similar coefficient of 0.85 as the standard,all varieties can be divided into three categories.GroupⅠincluding Longping,Yuanbao,Yutangba embankments,the Se mine enriched area of Yutangba,Lvcongpo;GroupⅡincluding Taiping and Wuliping;Shuanghe of Enshi City is divided into a class of independent.This study can provide a reference and basis for the identification of Dipsacus asper.

This article studied the improvement of rapid propagation system for Dendrobium candidum.The results showed that the best medium for the axillary bud induction of Dendrobium candidum was MS＋NAA 1.0 mg·L-1＋6-BA 3.0 mg·L-1,induction rate was 95%.Adding 2 g·L-1 activated carbon or 2.5 g·L-1 Vitamin C to the medium can effectively prevent the explants browning.The improved rooting medium was 1/2MS＋IBA 2.0 mg·L-1,and the rooting rate was over 90%.