采用高效液相色谱(HPLC)法,对3个莲藕主栽品种根状茎膨大过程中内源激素、水杨酸(SA)和多胺含量进行了分析.结果表明:玉米素(ZT)在1节期及3节期的第1节段含量最高;GA3含量在1节期即快速上升,IAA和SA则在2节期后才快速升高,GA3、IAA和SA在3节期均达最大值;膨大过程中3品种IAA和SA含量均呈极显著正相关;ABA含量在3节期的第1节段最高,第2节段最低;成熟期大幅升高.精胺(Spm)和腐胺(Put)在膨大过程中含量高,尸胺(Cad)和亚精胺(Spd)含量较低.
随着农村产业结构的调整,许多水乡农民利用丰富的淡水资源挖塘养鱼,经过多年的高密度养殖,大量投放的精饲料和青饲料未被完全取食而沉积,加上鱼排泄的粪便,在鱼塘底部形成一层富含有机质的淤泥,使鱼塘变得越来越浅,而且淤泥中病菌繁殖,水土逐渐恶化,出现鱼类疾病重发、渔业减产等鱼类连作障碍,经济损失极为惨重[1].这类鱼塘地势低洼,难以改造成农田,导致大面积鱼塘无法合理利用,严重影响了农民的经济收入.
【Objective】The objective of this paper is to study the relationship of starch contents with its related key synthease’s activities in lotus rhizome.【 Method 】 Using four lotus cultivars,the activity changes of adenosine diphosphoglucose pyrophosphorylase(ADPGPase),starch synthase(SSase) and starch branching enzyme(Q-enzyme) in rhizome during the development and their relationships with starch synthesis were studied.【Result】The peaks of fructose,glucose and total soluble sugar,sucrose content reached at early,middle,end stage of rhizome development,respectively.Total starch,amylose and amylopectin content in rhizome increased quickly after middle stage,total starch content reached 11.1% to 12.7% in fresh rhizome at end stage,and 70% of total starch was amylopectin.ADPGPase and SSase activities at the same development stage in different cultivar’s rhizome were obviously different,but Q-enzyme activity had little difference.During rhizome development SSase activity peak appeared earlier than ADPGPase and Q-enzyme at early stage,while ADPGPase and Q-enzyme activity peaks appeared at middle stage.ADPGPase activity at middle stage and SSase activity at early stage were very significantly and significantly correlated with total starch content at end stage in rhizome,co-efficients were 0.9830(P 0.01;n=12) and 0.8458(P0.05;n=12),respectively.Q-enzyme activity at middle stage was significantly correlated with amylopectin content at end stage,with co-efficient 0.7690(P 0.05;n=12).【Conclusion】Total starch content in matured lotus rhizome was determined by ADPGPase and SSase activities at middle and early stage;amylopectin content was determined by Q-enzyme activity at middle stage during lotus rhizome development.
