Transgenic expression of Cre recombinase driven by a specific promoter is normally used to conditionally knockout a gene in a tissue- or cell-type-specific manner. In αMHC-Cre transgenic mouse model, expression of Cre recombinase is controlled by the myocardial-specific α-myosin heavy chain (αMHC) promoter, which is commonly used to edit myocardial-specific genes. Toxic effects of Cre expression have been reported, including intro-chromosome rearrangements, micronuclei formation and other forms of DNA damage, and cardiomyopathy was observed in cardiac-specific Cre transgenic mice. However, mechanisms associated with Cardiotoxicity of Cre remain poorly understood. In our study, our data unveiled that αMHC-Cre mice developed arrhythmias and died after six months progressively, and none of them survived more than one year. Histopathological examination showed that αMHC-Cre mice had aberrant proliferation of tumor-like tissue in the atrial chamber extended from and vacuolation of ventricular myocytes. Furthermore, the αMHC-Cre mice developed severe cardiac interstitial and perivascular fibrosis, accompanied by significant increase of expression levels of MMP-2 and MMP-9 in the cardiac atrium and ventricular. Moreover, cardiac-specific expression of Cre led to disintegration of the intercalated disc, along with altered proteins expression of the disc and calcium-handling abnormality. Comprehensively, we identified that the ferroptosis signaling pathway is involved in heart failure caused by cardiac-specific expression of Cre, on which oxidative stress results in cytoplasmic vacuole accumulation of lipid peroxidation on the myocardial cell membrane. Taken together, these results revealed that cardiac-specific expression of Cre recombinase can lead to atrial mesenchymal tumor-like growth in the mice, which causes cardiac dysfunction, including cardiac fibrosis, reduction of the intercalated disc and cardiomyocytes ferroptosis at the age older than six months in mice. Our study suggests that αMHC-Cre mouse models are effective in young mice, but not in old mice. Researchers need to be particularly careful when using αMHC-Cre mouse model to interpret those phenotypic impacts of gene responses. As the Cre-associated cardiac pathology matched mostly to that of the patients, the model could also be employed for investigating age-related cardiac dysfunction.

Supplementary Figure 1. An overview of the study workflow; Supplementary Figure 2. The principal components analyses (PCA) of samples in the discovery stage and reference samples from the 1000 Genomes Project data. Supplementary Figure 3. Manhattan plots of the genome-wide P values from the association tests on HBV-related HCC. Supplementary Figure 4. Quantile-quantile plots of the observed P values from the association tests on HBV-related HCC. Supplementary Figure 5. Forest plots for rs10272859 across all studies. Supplementary Figure 6. Expression levels of CDK14 mRNA were significantly higher in HCC tissues compared with adjacent non-tumor liver tissues. Supplementary Figure 7. The at-risk G allele of rs10272859 was significantly associated with higher mRNA levels of CDK14 in liver tissues based on the TCGA data. Supplementary Figure 8. The SNPs at 7q21.13 interact physically with the promoter region of CDK14 in GM12878 cells. Supplementary Figure 9. Kaplan-Meier estimates of the overall survival time for the HCC patients stratified by CDK14 mRNA expression levels. Supplementary Figure 10. Kaplan-Meier estimates of disease-free survival time for patients with HBV-related HCC stratified by genotypes of rs10272859. Supplementary Table 1. Summary description of the populations used in this study. Supplementary Table 2. Summary of the quality controls in proband-parent trios. Supplementary Table 3. Summary of SNP imputation in the discovery stage. Supplementary Table 4. The amount of SNPs with various P values in TDT for the 189 trios in the discovery stage. Supplementary Table 5. Summary of the SNPs that have been reported significantly associated with HBV-related HCC in previous GWASs. Supplementary Table 6. Summary of SNPs that have been reported to be significantly associated with HBV-related phenotypes in previous GWASs. Supplementary Table 6. Summary of SNPs that have been reported to be significantly associated with HBV-related phenotypes in previous GWASs (Continued). Supplementary Table 7. Summary of the top 15 SNPs in the discovery stage. Supplementary Table 8. Primers and probes used in this study. Supplementary Table 9. Summary of the replication studies for the 14 SNPs newly identified in the discovery stage. Supplementary Table 10. Stratification analyses of rs10272859 by gender and age. Supplementary Table 11. The associations between genotypes of rs10272859 and mRNA levels of nearby genes. Supplementary Table 12. The predicted functional relevance of rs10272859 and the other 74 SNPs in strong linkage disequilibrium with rs10272859 at the ~110 Kb region of 7q21.13. Supplementary Table 13. Details for the genotypes of rs10272859 and the prognosis of patients with HBV-related HCC. Supplementary Table 14. Multivariate analyses of overall survival time and disease-free survival time of patients with HBV-related HCC. Supplementary Table 15. Genotype distribution of rs10272859 across TNM stages in patients with HBV-related HCC. Supplementary Table 16. Powers for various genetic effects and various minor allele frequencies. Supplementary Table 17. The allele and genotype frequencies of rs10272859 in different populations.

Tumor-associated macrophages (TAMs) are essential components of the immune cell stroma of hepatocellular carcinoma. TAMs originate from monocytic myeloid-derived suppressor cells, peripheral blood monocytes, and kupffer cells. The recruitment of monocytes to the HCC tumor microenvironment is facilitated by various factors, leading to their differentiation into TAMs with unique phenotypes. TAMs can directly activate or inhibit the nuclear factor-κB, interleukin-6/signal transducer and signal transducer and activator of transcription 3, Wnt/β-catenin, transforming growth factor-β1/bone morphogenetic protein, and extracellular signal-regulated kinase 1/2 signaling pathways in tumor cells and interact with other immune cells via producing cytokines and extracellular vesicles, thus affecting carcinoma cell proliferation, invasive and migratory, angiogenesis, liver fibrosis progression, and other processes to participate in different stages of tumor progression. In recent years, TAMs have received much attention as a prospective treatment target for HCC. This review describes the origin and characteristics of TAMs and their mechanism of action in the occurrence and development of HCC to offer a theoretical foundation for further clinical research of TAMs.

Supplementary Data, including Sup. Tables 1-5 and Sup. Figures 1-30.

Objectives. Previous experiments have shown that growth factor receptors play important role in tumor proliferation, metastasis, and therapeutic effect of chemotherapeutic drugs. At the same time, forkhead box D1 (FOXD1) plays an important role in a variety of signal transmission, but its expression profile was known little about head and neck cancer. The purpose of this experiment was to explore the regulation of FOXD1 on the tumor progression of head and neck cancer and to explore the correlation of FOXD1 on the expression of growth factor receptors (EGFR). Methods. The bioinformatics online database analyzed the expression of FOXD1 and EGFR in tumor tissues and nontumor tissues. Real-time quantitative PCR was used to detect the FOXD1 and EGFR expression in 45 tumor tissues and 15 nontumor tissues. The plasmid was used to construct FOXD1 overexpressing head and neck squamous cell cancer lines and observe the clonal formation and invasion of tumor cells under the intervention of EGFR-specific antibody—cetuximab. Results. The expression of FOXD1 and EGFR in tumor tissues was higher than that in nontumor tissues. The higher expression of FOXD1 and EGFR was not conducive to the prognosis of patients. The expression of FOXD1 and EGFR was positively correlated, and immunohistochemical analysis showed the high expression of FOXD1 and EGFR has close relation to the advanced stage of the tumor. In vitro cell experiments proved that overexpression of FOXD1 can partially offset the cloning ability of cetuximab on head and neck tumor cells. Conclusion. FOXD1 has an important regulatory role in the progression of head and neck cancer, and its abnormally high expression was not conducive to the prognosis of cancer patients. FOXD1 can regulate the expression of growth factor receptors in head and neck cancer, which provides a new idea for the better use of tumor growth factor receptor-specific antibodies for collaborative therapy.

Liver hepatocellular carcinoma (LIHC) remains a global health challenge with poor prognosis and high mortality. FKBP1A was first discovered as a receptor for the immunosuppressant drug FK506 in immune cells and is critical for various tumors and cancers. However, the relationships between FKBP1A expression, cellular distribution, tumor immunity, and prognosis in LIHC remain unclear. Here, we investigated the expression level of FKBP1A and its prognostic value in LIHC via multiple datasets including ONCOMINE, TIMER, GEPIA, UALCAN, HCCDB, Kaplan–Meier plotter, LinkedOmics, and STRING. Human liver tissue microarray was employed to analyze the characteristics of FKBP1A protein including the expression level and pathological alteration in cellular distribution. FKBP1A expression was significantly higher in LIHC and correlated with tumor stage, grade and metastasis. The expression level of the FKBP1A protein was also increased in LIHC patients along with its accumulation in endoplasmic reticulum (ER). High FKBP1A expression was correlated with a poor survival rate in LIHC patients. The analysis of gene co-expression and the regulatory pathway network suggested that FKBP1A is mainly involved in protein synthesis, metabolism and the immune-related pathway. FKBP1A expression had a significantly positive association with the infiltration of hematopoietic immune cells including B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells. Moreover, M2 macrophage infiltration was especially associated with a poor survival prognosis in LIHC. Furthermore, FKBP1A expression was significantly positively correlated with the expression of markers of M2 macrophages and immune checkpoint proteins such as PD-L1, CTLA-4, LAG3 and HAVCR2. Our study demonstrated that FKBP1A could be a potential prognostic target involved in tumor immune cell infiltration in LIHC.

BackgroundAldehyde dehydrogenase (ALDH) 1 is an important enzyme involved in the regulation of several cellular mechanisms via aldehyde detoxification. High ALDH1 levels were correlated with tumorigenesis and stemness maintenance in cancer.MethodsWe used UALCAN, Human Protein Atlas, Kaplan–Meier plotter, TISIDB, TIMER, and KOBAS databases to investigate the expression and role of ALDH1 in thyroid cancer progression. In addition, quantitative real-time polymerase chain reaction was performed to detect the expression of the target genes in thyroid cancer cell lines and cancer tissues.ResultsExpression of ALDH1A1/B1 was significantly decreased based on individual cancer stages and tumor histology, and high levels of ALDH1A1/B1 were associated with poor overall survival in thyroid cancer patients. Moreover, ALDH1A1/B1 expression was negatively correlated with immune-stimulating genes, major histocompatibility complex, chemokines, and receptors.ConclusionsThese results suggest that ALDH1A1/B1 might serve as potential prognostic biomarkers for thyroid cancer diagnosis.

Background: Hepatocellular carcinoma (HCC) is characterized by rapid progression, high recurrence rate and poor prognosis. Early prediction for the prognosis and immunotherapy efficacy is of great significance to improve the survival of HCC patients. However, there is still no reliable predictor at present. This study is aimed to explore the role of centromere protein L (CENPL) in predicting prognosis and its association with immune infiltration in HCC. Methods: The expression of CENPL was identified through analyzing the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) data. The association between CENPL expression and clinicopathological features was investigated by the Wilcoxon signed-rank test or Kruskal Wallis test and logistic regression. The role of CENPL in prognosis was examined via Kaplan-Meier method and Log-rank test as well as univariate and multivariate Cox regression analysis. Besides, in TIMER and GEPIA database, we investigated the correlation between CENPL level and immunocyte and immunocyte markers, and the prognostic-related methylation sites in CENPL were identified by MethSurv. Results: CENPL had a high expression in HCC samples. Increased CENPL was prominently associated with unfavorable survival, and maybe an independent prognostic factor of worse overall survival (OS), disease-specific survival (DSS), disease-free interval (DFI), progression-free interval (PFI). Additionally, CENPL expression was significantly correlated with immune cell infiltration and some markers. CENPL also contained a methylation site that was notably related to poor prognosis. Conclusions: Elevated CENPL may be a promising prognostic marker and associate with immune infiltration in HCC.

Endoscopic techniques are promising in breast surgery. In order to create working space, liposuction is widely used in video-assisted breast surgery (VABS). However, the use of liposuction is likely associated with side effects that may partly limit the application of VABS. Therefore, a new technique of endoscopic axillary lymphadenectomy without prior liposuction was developed by our group. A total of 106 female patients underwent VABS, with special adaptation of the video-assisted surgical procedures previously described. Differing from other endoscopic surgery techniques, our adaptations of VABS included the selection of the working instruments, trocar placement, creation of working space, order of axillary lymph node dissection and method of mastectomy. The operative time was 50-180 min (mean, 85.5 min). The intraoperative blood loss ranged from 20 to 100 ml (mean, 48 ml). The mean lymph node number harvested was 11.5 (range, 6-31). No serious intra- or postoperative complications were recorded. There was no axillary tumor relapse, trocar site tumor implantation or upper limb edema. Without prior liposuction, our new technique of VABS reduced the blood loss volume, endoscopic surgery time, total volume of drainage fluid and, most importantly, the risk of port-site metastases. This new technique appears to have great clinical potential and good prospects for future endoscopic breast surgery development.

Hepatocellular carcinoma (HCC) is a common cause of cancer-related death worldwide, and its incidence continues to increase. However, the mechanism underlying the development and progression of HCC remains unknown. The suppressor of cytokine signaling 2 (SOCS2) is a member of the SOCS family and influences the carcinogenesis of multiple types of tumors, but the biological roles of SOCS2 in HCC remain unclear. In this study, we found that SOCS2 expression was reduced in HCC tissues compared with matched noncancerous liver tissues. Moreover, decreased SOCS2 expression was significantly associated with the presence of intrahepatic metastasis and high histological grade in HCC patients. Colony formation assays and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays demonstrated that overexpression of SOCS2 or knockdown of endogenous SOCS2 did not significantly affect cell proliferation and tumorigenicity in HCC cells in vitro and in vivo. However, SOCS2 overexpression significantly inhibited the migration and invasion of HCC cells in vitro and inhibited metastasis in vivo. Consistent with these findings, the knockdown of endogenous SOCS2 enhanced migration and invasion in HCC cells in vitro. Our study demonstrated that SOCS2 inhibited human HCC metastasis, and SOCS2 might provide a new potential therapeutic strategy for treating HCC.

目的：观察干扰素-α联合窄谱中波紫外线治疗皮肤 T 细胞淋巴瘤的临床疗效。方法我院2012年1月至2013年12月期间所收治的皮肤 T 细胞淋巴瘤患者中选取20例作为此次研究对象，随机分为观察组和对照组，两组患者的年龄、性别等一般情况比较没有明显的差异，有可比性，P>0.05，具有统计学意义。结果观察组患者的治疗有效率为90.0%，对照组患者的治疗有效率为60.0%，观察组患者治疗有效率明显高于对照组患者，P<0.05，差异具有统计学意义。结论对于皮肤 T 细胞淋巴瘤患者，采用干扰素-α联合窄谱中波紫外线治疗，有助于提高患者治疗总有效率，安全性较高，具有一定的应用价值和治疗优势，值得大力推广使用。

We investigated the levels of target lymphocyte subsets in peripheral blood lymphocyte samples from patients with hepatocellular carcinoma (HCC). A total of 715 high-risk patients with primary HCC were recruited in Guangxi, China as the case group. The control group included 100 patients who received health examinations at the same hospital during the same period. Fasting elbow venous blood (10 mL) was collected from each participant, and flow cytometry was used to detect the levels of NK cells and CD3(+), CD4(+) and CD19(+) T cells in peripheral blood samples. All included patients with prmary HCC were treated by surgical resection, and followed up for one year. The levels of CD19+ and NK cells were lower in cases than in controls (both P < 0.05). In addition, the level of CD8(+) cells was greater in the case group than in the control group (P < 0.05). In the high-HCC-risk population, CD8(+), CD19(+) and NK cell levels all differed between male and female patients, patients in TNM stages I-II and stages III-IV, patients with and without extrahepatic metastasis, and patients with and without HBV infection (all P < 0.05). After follow-up, detected recurrence and survival rate was 33.71% and 83.64%, respectively. CD8(+) levels was reduced following surgical resection, whereas the levels of CD19(+) and NK cells were increased (all P < 0.05). In conclusion, altered levels of CD8(+), CD19(+) and NK cell levels may be used as reference values for monitoring immune function in certain populations with high HCC risk, and as potential evidence for the clinical diagnosis and treatment of HCC.

To investigate the treatment methods of retaining reproductive function in cesarean scar pregnancy. Clinical datus of 46 casesSin our hospital during 2000―2014Swere analyzed retrospectively.SThere were 45 cases of first-trimesterSpregnancy andS1 case of second-trimester pregnancy. 13 casesS(28.9 %)Sof first-trimesteSpregnancy were misdiagnosed and had received artificial abortion before hospitalization. According to CSP classificationS,S19Scases were type I,S13 cases were Stype II,Sall cases wereSsuccessful inSretaining the uterus. 63.1 % of type IScases receivedSuterine curettage under ultrasoundSmonitoring,S21%Sof type I casesSreceivedSuterineScurettageSafter uterine artery embolizationS(UAE)S. 84.6 %Sof type IIScases received surgery in which lesion were resected and uterine scar were repaired. The csp patient of second-trimester pregnancy suffered from thrombosis of lower extremity and rebleeding 30 days after UAE, she received the surgery of resecting lesion. Conclusion: STheSkeySforStheSconservativeStreatmentSSofS CSPSSis early diagnosis and early treatment. TreatmentSshouldSbeSindividualizedSaccording to CSP clinicSclassification ,β-HCG and so on.Furthermore, there are still someSrisk of UAE.WeSshould be careful to select UAESfor patients.

目的 探讨鼻咽癌患者外周血淋巴细胞亚群和NK细胞活性与治疗前后EB病毒IgA/VCA抗体水平高低、是否转移及不同临床分期的关系及其意义.方法 采用流式细胞术(FCM)检测58例鼻咽癌患者治疗前后外周血淋巴细胞亚群和NK细胞的百分含量.结果 ①鼻咽癌患者治疗后和治疗前比较,CD8+细胞水平显著升高,CD4+和CD19+细胞水平和CD4+/CD8+的比值显著下降,差异有统计学意义(P＜0.05),CD3+的差异无统计学意义(P＞0.05);②EB病毒IgA/VCA抗体高滴度和低滴度的鼻咽癌患者比较,在治疗前,NK细胞和CD19+细胞水平显著升高,差异有统计学意义(P＜0.05),其余指标差异无统计学意义(P＞0.05);在治疗后,各项指标差异无统计学意义(P＞0.05);③有淋巴结转移和无淋巴结转移的鼻咽癌患者比较,在治疗前,NK细胞和CD19+细胞水平显著升高,差异有统计学意义(P＜0.05),其余指标差异无统计学意义(P＞0.05);在治疗后,各项指标差异无统计学意义(P＞0.05);④Ⅲ～Ⅳ期和Ⅰ～Ⅱ期的鼻咽癌患者比较,在治疗前,CD19+细胞水平显著升高,差异有统计学意义(P＜0.05),其余指标差异无统计学意义(P＞0.05);在治疗后,各项指标差异无统计学意义(P＞0.05).结论 鼻咽癌患者机体细胞免疫状态与机体是否存在局部淋巴结和远处转移无明显相关性,但在进行治疗后机体细胞免疫抑制状态较治疗前明显改善.

Nasopharyngeal carcinoma (NPC) is the most commonly diagnosed head and neck malignancy and is prevalent worldwide. Previous studies have demonstrated the antitumor properties of cepharanthine hydrochloride (CH) in several human cancer cells. However, the action of CH in NPC cells has yet to be determined. In the present study, we investigated the effects of CH in human NPC cell lines including CNE-1 and CNE-2 on cell growth and apoptosis in vitro. Using MTT and ATP-tumor chemosensitivity assays it was found that CH inhibited cell viability. Additionally, flow cytometric and analysis electron microscopy revealed the inhibition of cell cycle progression and reduction of apoptosis, respectively, in human NPC cell lines including CNE-1 and CNE-2 in vitro. To identify the potential action mechanisms of CH, the cDNA microarray analysis results were confirmed by quantitative PCR analysis using a number of genes, including CDKN1A/P21, NR4A1/TR3 and DAXX. In total, 138 upregulated and 63 downregulated genes in CNE-2 cells were treated with CH. According to their biological function, the genes were classified as: i) cell cycle-related genes; ii) DNA repair‑related genes; iii) apoptosis-related genes and iv) nuclear factor-κB (NF-κB) transcription factors signal pathways. The results of the present study showed that CH is a potential therapeutic agent against human NPC, and provide rational explanations and a scientific basis for the study of the development of CH in the treatment of NPC.

Objective: explore expression of peripheral blood t lymphocytes and nK cell levels in patients with liver cancer and hepatitis B and its significance. Method: By fiow cytometry (fcM) detected 47 cases of liver cancer, 58 cases of hepatitis B patients and 42 healthy percentage of peripheral blood t lymphocytes and nK cells. Results: either hepatitis B or liver cancer patient group and healthy people of cd3 + and cd4 + decline, cd8 + increased nK levels also decreased, but no significant difference in liver cancer decline; HBV dna low copy group and the high-copy group,and of HBV dna (-) group, or healthy people compare both of cd3 + cd4 +, and cd4 + / cd8 + the ratio decreased cd8 + increased nK level also decreased compared between low-copy group and high copy of the group, as well as of HBV of dna (-)group and healthy people, the indicators were not statistically different; Hcc patients, HBV dna (-) group of HBV dna low copy and high copy group, pairwise comparisons between groups, the indicators areno statistically significant difference. Conclusion: compared with healthy people, hepatitis B virus infection and liver cancer cause varying degrees lead to immune dysfunction, indirectly hepatitis B virus infection play a role in the process of the development of primary liver cancer.

鼻咽癌是一种在我国广东和广西地区高发的肿瘤，一般与饮食、地域、遗传、EB病毒（ EBV ）的感染有关〔1〕。鼻咽癌的临床分期对治疗方式以及预后有着重要的作用，即使是同一分期的鼻咽癌，其预后也有一定的差别〔2〕，所以发现、选择一种或者几种判断肿瘤进展情况的分子标志物显得尤为重要。本文就血管内皮生长因子（VEGF）以及生存素（survivin）与鼻咽癌的相关性研究进展进行综述。

BACKGROUND:Genetic variations in microRNAs may alter their processing, expression, and binding to target mRNAs, consequently affecting many cancer-related biological processes. Recently, a polymorphism rs11614913 in MIR196A2 was shown to affect the processing of the precursor microRNA into its mature forms and the repertoire of target mRNAs with which it interacts. We examined whether this polymorphism was relevant to the risk of occurrence or progression of nasopharyngeal carcinoma (NPC) in the Chinese population.METHODS:We genotyped the MIR196A2 rs11614913 in a case-control study of 1084 patients with NPC and 1036 cancer-free controls using the TaqMan assay. The genetic associations with the risk of occurrence and progression of NPC were analyzed by logistic regression.RESULTS:We observed a significantly increased occurrence of NPC associated with the rs11614913 T allele (odds ratio [OR]=1.15, 95% confidence interval [CI]=1.02-1.32, p=0.026) compared with the C allele. The T allele was also significantly associated with the advanced local tumor invasion (T₃+T₄ vs. T₁+T₂; OR=1.27, 95% CI=1.04-1.54, p=0.015) and advanced lymph node metastasis (N₂+N₃ vs. N₀+N₁; OR=1.23, 95% CI=1.02-1.49, p=0.031) of NPC compared with the C allele. Furthermore, stratified analysis indicated that the increased susceptibility to advanced lymph node metastasis of NPC related to the T allele was more pronounced in patients with a positive family history (N₂+N₃ vs. N₀+N₁; p=0.016, test for homogeneity).CONCLUSIONS:Our study suggests that the functional polymorphism rs11614913 in the MIR196A2 gene may contribute to the risk of occurrence and progression of NPC in the Chinese population.

p73, a structural and functional homolog of p53, plays an important role in modulating cell cycle control and apoptosis. We examined whether the p73 G4C14-to-A4T14 polymorphism was related to the risk of nasopharyngeal carcinoma (NPC) among Chinese populations. The G4C14-to-A4T14 polymorphism was genotyped in 593 NPC cases and 480 controls, and in 102 NPC trios. Logistic regression analysis and transmission/disequilibrium tests (TDT) were performed to evaluate whether there was an association between the polymorphism and NPC, respectively. Functional analyses were conducted to verify the biological relevance of the polymorphism. We observed that compared with the GC/GC genotype, the genotypes containing AT allele (GC/AT + AT/AT genotypes) were associated with significantly increased susceptibility to NPC [odds ratio (OR) = 1.51; 95% confidence interval (CI) = 1.16-1.95; P = 0.002]. Furthermore, compared with the GC/GC genotype, the GC/AT + AT/AT genotypes were significantly associated with the advanced lymph node metastasis (OR = 1.47; 95% CI = 1.02-2.11; P = 0.041). A significantly greater than expected transmission of the AT allele from heterozygous parents to offspring was also observed (P = 0.049) using the TDT. By using the TdT-mediated dUPT-biotin nick end labeling assay, we observed lower apoptosis in NPC tissues from the AT allele carriers compared with that from non-carriers. Furthermore, the relative TAp73 RNA levels of the AT allele were lower than those of the GC allele in heterozygous cells. Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may play a role in mediating the susceptibility to NPC in Chinese populations.

腹腔镜手术在外科领域得到迅速发展,成为目前主要的手术方式之一.为进一步减少腹腔镜手术的创伤和提高美容效果,自然腔道内镜手术(natural orificetransluminal endoscopic surgery,NOTES)成为内镜手术的热点[1],经脐单孔多通道腹腔镜(laparoendoscopic single-site,LESS)技术被逐渐应用于临床.我科于2012年7月、9月完成2例单孔多通道腹腔镜输卵管通液检查手术,效果满意,现总结报告如下.