Jute is an important fiber crop in textile industry and paper manufacture. The objective of this study is to establish sequence related amplified polymorphism( SRAP) fingerprints of 36 representative jute varieties at home and abroad. A total of 50 SRAP was used to survey the polymorphism,the result showed 34 pairs of SRAP primers could amplify polymorphism bands and revealed 1845 polymorphic loci in total,with an average of 51.25 polymorphic bands per primer pair. Then 14 pairs core primers were selected. They could distinguish the 36 jute varieties to establish the DNA fingerprints,in which each variety had its unique fingerprinting pattern.

Kenaf (Hibiscus cannabinus L.) is an important economic crop in China.To identify germplasm resources and establish the data base of DNA fingerprintings,we adopted six kenaf germplasm resources to screen 80 ISSR primers,20 of which were polymorphic.Then 20 ISSR primers were used to amplify 84 kenaf germplasm resources introduced and preserved from home and abroad.Totally 230 bands were produced,the average number of DNA bands amplified by each primer was 11.5,and the number of polymorphic DNA bands was 185.The polymorphic proportion of DNA bands was 80.43%,which indicated the abundant genetic diversity of kenaf germplasm resources preserved.Based on the DNA bands amplified from 84 kenaf germplasm resources,the Excel data base for PCR amplified bands of the kenaf germplasm resources was established.According to the principle of uniqueness of the fingerprintings,the DNA fingerprints of 82 kenaf germplasm were constructed with five ISSR primers (UBC813,UBC825,UBC836,UBC888,and UBC889) selected from 20 polymorphic primers,using the analyzing software designed by our laboratory programmed and based on image manipulation.The fingerprintings provide a basis of the molecular identification of kenaf germplasm resources.In this research,the DNA fingerprintings system was proved to be feasible and reliable.

论述了MCS-51系列单片机堆栈特点、开辟方法和应用技巧,并指出了堆栈的不当使用及其原因.