[目的]探讨胃癌患者血清趋化因子L20(CCL20)、热休克蛋白70(HSP70)水平及临床意义.[方法]在本院和苏州附属第二医院治疗的胃癌患者92例(胃癌组),同时选取健康志愿者100例作为对照组,比较两组血清CCL20、HSP70水平并分析胃癌患者血清CCL20与HSP70水平的相关性.[结果]胃癌组血清CCL20、HSP70水平分别为(44.12±9.47)pg/mL和(2.40±0.65)pg/L,明显高于对照组(P<0.05);Ⅲ～Ⅳ期、有淋巴结转移、HP感染阳性胃癌患者血清CCL20和HSP70水平明显高于Ⅰ～Ⅱ期、无淋巴结转移、HP感染阴性患者(P<0.05);血清CCL20与HSP70呈正相关(r=0.433,P<0.05).[结论]胃癌患者血清CCL20、HSP70水平明显升高,且与患者临床分期、淋巴结转移及HP感染有一定的关系.
Objective Infection status one case of low concentrations of HIV RNA window period blood donor was confirmed by combined serological and molecular biological methods. Methods On serological ELISA results qualified blood specimens for nucleic acid detection, 1 case of low concentration of HIV window period were found via the method of qualitative and quantitative detection of nucleic acids (NAT), the donor's samples in different periods of blood specimens of HIV viral load were detected. Results The first time blood donor samples tested negative by ELISA serology, nucleic acid reactive qualitative test, followed by quantitative nucleic acid testing is less than 20 copies / mL; for follow-up samples collected serological and nucleic acid testing, final confirmation serum turn positive. Conclution The blood donor is a very low viral load of 1 HIV window period of blood donors, NAT detection technology and ELISA detection combined to further protect the safety of clinical use of blood.
目的：探讨苏州地区无偿献血员庚型肝炎病毒（HGV ）感染的血清流行病学现状及其输血安全。方法随机收集2013年4月至2014年11月该站经过血液筛查后的标本3520份，对其分别进行乙型肝炎表面抗原（HBsAg ）、抗丙型肝炎病毒（HCV）、抗HGV酶联免疫吸附试验（ELISA）检测，再将3种ELISA检测的阳性标本进行HGV RNA检测确认。结果3520份标本经HBsAg、抗HCV、抗HGV检测阳性数分别为10、9、3份，经HGV RNA检测确认阳性数为1份。结论苏州地区无偿献血员HGV感染率极低，无血清流行病学意义，但仍应采取有效措施，确保临床用血安全。
目的 探讨T淋巴细胞亚群、自然杀伤(NK)细胞及辅助T细胞(Th) 1/Th2细胞因子与先兆流产发病的关系.方法 采用免疫荧光标记和流式细胞术检测40例不明原因先兆流产患者(A组)和25例正常妊娠妇女(B组)外周静脉血CD3+T、CD4+T、CD8+T细胞及NK细胞,采用ELISA法检测血清中Th1型细胞因子(TNF-α和IL-2)及Th2型细胞因子(IL-4和IL-10)的表达水平.结果 两组CD3+T、CD4+T、CD8+T细胞百分比及CD4+/CD8+比较无统计学差异(P＞0.05).A组NK细胞百分比低于B组[(8.7±3.2)％ vs.(19.6±8.1)％](P＜0.05).A组血清Th1型细胞因子TNF-α和IL-2表达水平高于B组(P＜0.05).结论 细胞免疫异常及Th1/Th2型细胞因子的病理性失衡与先兆流产的发生有关联.
Objective To compare the detection methods of hepatitis C virus in blood screening and to explore its application value.Methods 41 cases of anti-HCV positive samples detected by enzyme-linked immunosorbent assay(ELISA) in blood screening during August-December 2011 were collected and performed the confirmatory assay by Western blot.At the same time,two kinds of anti-HCV ELISA reagent provided by Intec and Ortho companies and Architect anti-HCV reagent were adopted for detection.The differences in the results among three methods were compared.Results The statistical analysis showed that the results detected by three methods had statistical difference.In the recombinant immunoblot assay measurement,35 cases were HCV positive and 6 cases were negative,which conformed to the results by the Architect chemiluminescence immunoassay.In the Intec enzyme immunoassay test,41 cases were all positive.In the Ortho enzyme immunoassay test,33 cases were positive and 8 cases were negative.Conclusion The Ortho reagent is more specific than the Intec reagent for the HCV detection,the chemiluminescence immunoassay detection is more specific than the enzyme immunoassay.The combined detection of the chemiluminescence immunoassay and the enzyme immunoassay can improve the detection rate of HCV positive in blood screening.The suspicious sample is further analyzed by the recombinant immunoblot assay.
目的比较临床检验中乙型肝炎病毒检测方法,并探讨其临床应用价值。方法收集2011年12月期间临床酶免HBV阳性样本共50例,使用PCR荧光探针法进行确证;同时用新创、Ortho两种HBV酶免试剂以及Architect HBV Reagent化学发光试剂进行检测,比较三者结果的差异。结果统计学分析显示,三种检测方法的检测结果有差异。PCR实验测出HBV阳性44例,阴性6例,与Architect化学发光法结果相符,新创酶免法测50例全为阳性,Ortho酶免法测阳性42例,阴性8例。结论 Ortho试剂对HBV检测的特异性高于新创试剂,化学发光法对HBV检测的特异性高于酶免法,化学发光法与酶免法联合检测能提高临床检验中HBV的阳性检出率,对可疑样本再作PCR实验分析。
<正>精子蛋白17(sperm protein 17,Sp17)是一种高度保守的睾丸组织特异性蛋白,与在卵细胞透明带上的乙酰肝素糖基进行结合,参与顶体反应,促进受精过程[1]。近年来,Sp17作为一癌瘤—睾丸(CT)抗原,其作为肿瘤疫苗的前景被广泛研究
