目的 了解苏州市无偿献血者Rh阴性的血清学表型和意外抗体分布情况.方法 应用血清学方法,对献血者进行Rh阴性筛选及确认、血清学表型鉴定及意外抗体筛选的检测分析.结果 在94 108 份标本中共确认Rh阴性578 例(0.61%),其中检出血清含有意外抗体的标本有8 例(1.38%),Rh阴性献血者中血清学表型分布:ccdee 339 例(58.65%),Ccdee 175 例(30.28%),CCdee 28 例(4.84%),ccdEe 23 例(3.98%),CcdEe 10 例(1.73%),CCdEe 1 例(0.17%),ccdEE 1 例(0.17%),CcdEE 1 例(0.17%).ABO血型分布的结果:A型169 例(29.24%),B型141 例(24.39%),O型192 例(33.22%),AB型76 例(13.15%).结论 苏州市无偿献血者稀有的Rh阴性人群的血清学表型和意外抗体筛查情况为血源管理和采供血单位资源共享提供支持.
目的：探讨苏州地区无偿献血员庚型肝炎病毒（HGV ）感染的血清流行病学现状及其输血安全。方法随机收集2013年4月至2014年11月该站经过血液筛查后的标本3520份，对其分别进行乙型肝炎表面抗原（HBsAg ）、抗丙型肝炎病毒（HCV）、抗HGV酶联免疫吸附试验（ELISA）检测，再将3种ELISA检测的阳性标本进行HGV RNA检测确认。结果3520份标本经HBsAg、抗HCV、抗HGV检测阳性数分别为10、9、3份，经HGV RNA检测确认阳性数为1份。结论苏州地区无偿献血员HGV感染率极低，无血清流行病学意义，但仍应采取有效措施，确保临床用血安全。
Objective To analyze the epidemic characteristics of volunteer blood donors with HIV infection in Suzhou city, in order to make countermeasures to recruit appropriate volunteer blood donors. Methods Infectious situation and population characteristics of volunteer blood donors with HIV infection were explored by a retrospective analysis method in Suzhou city between 2012 and 2013. Results Among 755 643 volunteer blood donors from 2002 to 2013, 51 donors were HIV infection, the positive rate was 6.75/100000, and the highest detection rate was found in 2013 (23.25/10万), following as 2012 for 9.71/100000, and the lowest was 1.66/100000. There was a statistically significant differ-ence among these years(χ2=37.96, P<0.01). 42 cases of 51 cases with HIV infection were men, the other 9 were women, sex ratio between male and female was nearly 5:1. The donors with 18 to 30 years old (60.79%) were predominant pop-ulation, unmarried people were as high as 62.75%, the staff were 56.86%, the donors with high school, technical sec-ondary school degrees and following degrees primarily accounted for 50.98%, and individual donors accounted for 70.59% of HIV infections. Conclusion There is an upward trend of HIV infection rate from voluntary blood donors in Suzhou in recent years. Blood collection organization should strengthen the health consultation of voluntary blood donors before the blood donation, especially among young adults, strict screening for HIV, which guarantee for clinical safety of blood transfusion.
脂肪血是临床输血及血液制品生产的禁忌,输注脂肪血可发生过敏、发热、脂肪栓塞等不良反应。用脂肪血分离蛋白及制品,不能保证产品的质量。随着医学事业发展临床用血不断增加,降低脂肪血引起的血液报废,保护血液资源非常重要。中华人民共和国国家标准:GB18469-2001全血及成份血质量标准为外观无重度乳糜。而脂肪血与甘油三脂（TG）密切相关[1]。全血快速干化学纸片法TG检测其操作简便快速已用于脂肪血筛检[2],但未有筛检参考值及应用的相关报道,笔者就此方面进行探
目的探讨血站血液筛查中阳性样本与其血辫检测的符合性研究。方法收集2011年4～7月期间血站血液筛查中ELISA4项(HBsAg、抗HCV、抗HIV、梅毒螺旋体抗体)检测结果阳性样本共188例,对其血辫(含血液保存液,-20℃保存)进行ELISA4项检测,比较二者结果的差异。结果统计学分析显示,188例阳性样本的血辫经检测有31例为ELISA4项全阴性,表明阳性样本与其血辫的ELISA4项检测结果有差异,弱阳性样本差异显著。结论血站血液筛查中阳性样本与其血辫的ELISA4项检测有显著差异,血液保存液会造成弱阳性标本漏检;应采取有效措施,确保临床用血安全。
OBJECTIVE To set up western blot method for the detection of syphilis antibodies and evaluate its effect. METHODS Gelatin particle agglutination test was conducted towards the following two groups,13 shares in Group 1 adopted Toluidine red non-heating test and enzyme-linked immunity and adsorption assay test;28 shares of spacious samples in Group 2 screened by double enzyme-linked immunity and adsorption assay test performed the gelatin particle agglutination test and confirmatory test and compared the above mentioned test with TP-WB method toward two groups. RESULTS The confirmatory results obtained through gelatin particle agglutination test were 6 cases positive,2 cases suspicious,5 cases negative and 12 cases positive,2 cases suspicious,14 cases negative;the positive rates were 46.2%,42.9%;while the results obtained through TP-WB were 11 cases positive,2 cases suspicious,and 18 cases positive,2 cases suspicious,8 cases negative;the positive rates were 78.4%,64.3%. CONCLUSION TP-WB method is simple in operation,easy to observe the results without special equipments,good at sensitivity and specificity,and more suitable for syphilis confirmatory test in all levels of laboratory.
全血快速干化学法丙氨酸氨基转移酶(ALT)筛检已广泛应用于街头无偿献血,为减少因ALT不合格的血液报废发挥有效作用.
