Objective:(1) To explore the difference of three kinds of adiponectin rs +16861194 (A / G ) genotype distribution in the type 2 diabetes patients and healthy people ; (2) To investigate the relationship of adiponectin rs+16861194 gene polymorphism with some biochemical indicators from T2DM and control.Methods:Selected 158 cases of type 2 diabetes patients as the experimental group, 132 cases of healthy people as the control group. The genotypes of adiponectin rs-16861194 in experimental group and the control group were detected; At the same time, fasting plasma glucose, triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) were measured seperately. Fasting insulin was measured by chemiluminescence and insulin resistance index was calculated.Results:①T2DM group and CON group adiponectin rs +16861194 genotype distribution were: AA (70.00%), AG (24.37%), G (5.63%) and AA (74.24%), AG (24.24%), GG (1.52%), the distribution showed no statistically significant difference (P> 0.05); ②Grouped by the genotype of adiponectin rs +16861194, the level of FBG, FIN, IRI, TG, CHOL, HDL-CH, LDL-CH showed no statistically significant difference (P> 0.05) between the genotypes in T2DM and CON group. ③ Compraring between T2DM group and CON group,.the level of FBG, FIN, IRI, TG, HDL in the AA type of T2DM group and CON group had significant difference (P<0.05), while the level of CHOL and LDL showed no statistically significant difference (P> 0.05) ; The level of FBG,IRI,TG,HDL in the AG+GG type of T2DM group and CON group had significant difference (P<0.05), while the level of FIN,CHOL,LOL showed no statistically significant difference (P> 0.05).Conclusions:(1) Three kinds of locus genotype distribution of rs +16861194 (A / G) had no significant difference in Type 2 diabetes patients and healthy people.(2) The genotype of adiponectin rs+16861194 had no effect on the level of FBG、FIN、IRI、TG、CHOL、HDL-CH、LDL-CH in type 2 diabetes patients and healthy people.

Objective To analyze unexpected antibody results of 1217 cases of blood transfusion patients from eight hospitals in Zengcheng ,and evaluate antibody distribution and compare different examination methods .Methods Antibody screen test and anti-body identification were simultaneously carried out with Tube Liss-IAT test and DiaMed-ID test,and the positive rate and sensitive rate of the two methods were compared .Results Seventy-two patients were found positive in antibody screen tests ,and the sensitive sam-ples of unexpected antibody identification of Tube Liss-IAT method were 65 cases(90.3%),and DiaMed-ID method tested 70 cases (97.2%).Conclusion Unexpected antibodies are mainly in the rhesus system ,and the result of DiaMed-ID method is more sensitive than that of tube method .

目的 评价幽门螺杆菌粪便抗原(HpSA)免疫快检卡的临床应用价值.方法 对300例上腹不适患者进行胃镜检查,采用快速尿素酶试验联合组织学检查作为金标准,选取Hp感染阳性180例,按照随机数字表法分为HpSA免疫快检卡组和14C-尿素呼气试验(UBT)组,每组各90例,给予1周三联治疗方案进行治疗,治疗后4周和12周分别采用HpSA免疫快检卡或14C-UBT进行检查,均与快速尿素酶试验联合组织学检查方法相对照.结果 HpSA免疫快检卡组86例为Hp阳性,灵敏度为95.6%,14C-UBT组87例为Hp阳性,灵敏度为96.7%.根除治疗后12周,HpSA免疫快检卡组71例根除成功,成功率为82.6%,14C-UBT组73例根除成功,成功率为83.9%.两组比较差异均无统计学意义(P>0.05).结论 HpSA免疫快检卡根除治疗前后检查Hp感染的特异度、灵敏度及总精确度与14C-UBT、金标准法相似;采用HpSA免疫快检卡进行检查,具有环保、操作简单、快速、无创、经济、结果稳定可靠、患者依从性好等优点,值得临床推广应用.

目的探讨乙型肝炎(简称乙肝)后肝硬化门静脉高压性胃病(PHG)患者抗幽门螺杆菌(Hp)感染治疗的临床意义。方法对60例临床及胃镜确诊为乙肝后肝硬化PHG患者(其中轻、重型各30例)分别应用粪便Hp抗原(HpSA)免疫快检卡检测Hp,同时对Hp感染阳性患者用三联疗法治疗1周,方案为洛赛克胶囊20 mg、克拉霉素0.5 g、阿莫西林1.0 g,每日2次,用药1周。4周后用同样方法复查Hp。结果60例患者中Hp感染阳性病例共39例,感染率为65.00%(39/60),其中PHG轻型者感染率为43.33%(13/30)、重型者感染率为86.67%(26/30),二者比较差异有统计学意义(P<0.05);用三联疗法抗Hp治疗的根治率为89.74%(35/39),其中PHG轻型根治率为92.31%(12/13)、重型根治率为88.46%(23/26),二者比较差异无统计学意义(P>0.05)。结论在乙肝后肝硬化患者中Hp感染可能参与PHG的发生和发展,且与PHG病变程度呈正相关;三联疗法根除Hp感染效果显著,有较好的效价比;HpSA免疫快检卡检测Hp具有快速、无创、简便、经济、标本易收集、结果可靠等特点,特别适宜在基层医院应用。

目的 检测结肠癌患者外周血CEA和CK20mRNA的表达水平及评估其对肿瘤转移的预测价值.方法 分别采用RT-PCR和传统定性PCR技术,检测结肠癌患者(85例)和健康志愿者(45例)外周血CEA和CK20mRNA的表达水平.结果 85例结肠癌患者外周血CEA和CK20mRNA的水平分别为(920±104)copies/ml和(532±91)copies/ml,健康志愿者未检测到CEA和CK20 mRNA表达;CEA和CK20 mRNA水平与Dukes分期、细胞分化程度相关,Logistic回归分析得随Dukes分期进展,肿瘤转移率逐渐增加(与A期相比OR值分别为1.087、1.983、2.876);RT-PCR检测灵敏度明显高于定性PCR.结论 RT-PCR检测结肠癌患者外周血CEA和CK20 mRNA表达水平灵敏度高,CEA和CK20 mRNA具有较好的肿瘤微转移预测价值.

目的 评价中性粒细胞体积分布宽度在急性细菌感染中的诊断价值.方法 采用VCS技术检测240例急性细菌感染实验组患者和120例对照组外周血白细胞计数(CBC)、中性粒细胞百分比(DC)和中性粒细胞体积分布宽度(NDW),比较三者对急性细菌感染的诊断价值.结果 实验组患者外周血CBC、DC、NDW均较对照组明显升高,两者差别具有统计学意义(P<0.05);NDW对急性细菌感染诊断效率高于CBC和DC.结论 与传统检测方法相比,VCS技术检测NDW可作为一项快速和准确的检测方法,对急性细菌感染具有较好临床应用价值,值得推广.

探讨HBV-DNA定量和定性聚合酶链反应的意义及其临床实用性.检测283例临床血清标本,其中125例用实时荧光定量PCR测HBV-DNA含量并比较不同乙型肝炎(乙肝)病型;另158例用定性PCR测HBV-DNA并用ELISA法规HBV血清标志物(HBVM),同时进行DNA定量及定性的对比研究.结果示HBV-DNA含量与乙肝病型无明显关系;DNA阳性率与乙肝病型及HBVM有关;HBV-DNA定量与定性相关性良好.FQ-PCR可真实反映HBV-DNA复制水平,有广泛的应用前景;定量与定性HBV-DNA检测相关性良好,在仅需了解HBV-DNA的复制与否的诊断中,可用定性PCR;对HBVM的意义应重新认识.