试验研究了废橡胶颗粒品质对橡胶混凝土抗压强度及对改性剂改性效果的影响.结果表明,未改性前,掺加外胎橡胶颗粒混凝土的抗压强度较低,而掺加内胎和杂制品橡胶颗粒混凝土的抗压强度较高;橡胶颗粒经NaOH溶液和KH570改性后,掺加外胎橡胶颗粒混凝土的抗压强度增幅较大,而掺加内胎和杂制品橡胶颗粒混凝土的抗压强度却大幅下降;对外胎橡胶颗粒而言,NaOH改性溶液的较优浓度为1%~20%,KH570的较优用量为0.5%~1.5%.
目的 对采自河南省临颍县麦田的土壤样品进行苯磺隆耐受菌的筛选和初步鉴定.方法 利用选择性培养基,经富集培养,从长期施用苯磺隆的麦田土壤中筛选苯磺隆耐受菌.采用基于16S rRNA基因序列的系统发育分析和生物学特性研究,对菌株进行初步分类鉴定.结果 共分离到耐受苯磺隆的纯菌12株,其中一株LYBRD3-3对苯磺隆耐受能力最强,苯磺隆耐受浓度为150 μg/L.经过对该菌形态观察、生理生化特征及基于16S rRNA基因序列的系统发育分析表明,LYBRD3-3与无色菌属的Achromobacter insolitus LMG 6003T (AY170847)序列具有最高的相似性,同源性为99.58%,初步鉴定该菌为无色菌属菌.结论 分离自麦田土壤的苯磺隆耐受菌为进一步筛选苯磺隆降解功能菌株提供菌种资源,进而为污染土壤的原位生物修复提供基础研究.
A method is developed for the quantitative analysis of tribenuron-methyl by reversed-phase high performance liquid chromatography,in MS medium,on C18 Column with uv-absobance detector.The samples of ahead processing design adopt dichloromethane extraction and revolving evaporator and methanol dissolve.The linear relation was very good in the range of 25~200 μg/mL(R2=0.999).The samples standard deviation was 0.082,the coefficients of variation was 0.16 %,and the average recovery was 100.86 %.This method can accurately determinate tribenuron-methyl content in the medium.