目的 探讨虚拟仿真实验项目与CBL及TBL相结合的混合式教学方法在“医学形态学实验”教学中的应用效果.方法 以2016级和2017级选课学生为研究对象,随机分为对照组和实验组,对照组采用传统教学方法,实验组采用混合式教学方法,分别进行“医学形态学实验”教学;课程结束后,对学生进行问卷调查及综合考核,比较两种方法的教学效果.结果 实验组学生的综合考核成绩显著高于对照组(P＜0.05);问卷调查显示,实验组学生对混合式教学方法满意度较高(P＜0.05).结论 在“医学形态学实验”教学中,混合式教学的教学效果明显优于传统教学方法.

0 引言 脑脂肪酸结合蛋白(BLBP/B-FABP,FABP7)通常在处于发育期中枢神经系统中的放射状胶质细胞和已分化的成年胶质细胞群例如神经胶质介膜细胞和Bergmann胶质细胞中表达[1-2],在成人中枢神经系统中的表达明显减少[3].有研究发现FABP7的表达对构建和维持放射状神经胶质纤维系统是必需的[4].乳腺癌细胞和脑细胞同样富含脂肪,我们通过观察FABP7基因对人乳腺癌MCF-7细胞增殖能力的影响,为进一步研究FABP7的功能提供依据.

Objective To construct a eukaryotic expression vector for the gene encoding human brain fatty acid binding pro-tein 7(FABP7)and observe its effect on the proliferation of human breast cancer MCF-7 cells.Methods Amplify FABP7 gene from stellate cell tumor tissue by RT-PCR,digest with BamHⅠand Hind Ⅲ and insert downstream to the promoter of linearized pcDNA3.1 vector.Tranfect the constructed recombinant plasmid pcDNA3.1-FABP7 to human breast cancer MCF-7 cells and determine the ex-pression of FABP7 mRNA by semi-quantitative RT-PCR.Count the cells 48,72 and 96 h after transfection respectively,and deter-mine the cell proliferation activity by MTT method and the change of cell cycle by flow cytometry.Results Both restriction analysis and sequencing proved that recombinant plasmid pcDNA3.1-FABP7 was constructed correctly.The count and A490 value of cells 48,72 and 96 h after transfection with pcDNA3.1-FABP7 were significantly lower,while the percentages of cells at G1 stage were signifi-cantly higher,than those with empty plasmid pcDNA3.1.Conclusion The eukaryotic expression vector for FABP7 gene was suc-cessfully constructed,which inhibited the proliferation of human breast cancer MCF-7 cells significantly.

Background: At present most chips use the glass film base by Cy3 and Cy5 mark, their price is so high that are only applied in scientific researches, and clinical application cannot be promoted massively. The topic-based group uses the nylon membrane as the carrier to manufacture gene chips, in order to obtain one kind of chip that has high sensitivity and accuracy, fast simple, may simultaneously examine many kinds of illnesses to get sick, and costs low. Objective: To investigate the feasibility of taking the nylon membrane as the carrier to obtain gene chip, and simultaneously optimize the quantity of the probe and the goal gene. Design, time and setting: Experiments at genetic engineering probe design were carried out from October 2005 to September 2006 in the laboratory of Lanzhou University Science and Technology Garden Baiyuan Gene Company. Materials: Fresh colon cancer samples were excised from Gansu Cancer Hospital between October 2005 and April 2006, while the normal tissues apart from tumor edge above 5-10 cm were taken as controls. The pathology confirmed that the cancer cell had not infiltrated. The nylon membrane belt (from Amersco Corporation) was positive charge. Methods: 200 mg cryopreserved tissues were taken out of liquid nitrogen, total RNA extraction was performed using Trizol method, and mRNA was purified through the Oligo dT chromatography. The first and the second chain of cDNA was synthesized with reverses transcription by using TaKaRa's M-MLV Rtase cDNA synthesis Kit (code D6130). The product was divided into two parts, a part as the template for the digoxin mark PCR, the obtained product served as the goal gene. Another part was taken as the template to manufacture probe. As for the resultant GAPDH, Actin, CyclinD1, the gene fragment PCR product was processed into the purification. Using the positively charged nylon membrane as the carrier, three kind of gene fragment at different density were taken as probes on the carrier, the obtained chip hybridizated separately with the different densities of digoxin mark goal gene. Results: When the goal gene (2.5 μ L and 1.25 μ L) was sampled and added with the hybrid fluid, the goal gene's density was so small as to increase the density on each probe, which was unable to reflect the correct colored tendency. When the goal gene was sampled 5 μ L and the probe density was 10-20 μ L, three kinds of probes exhibited a colored depth tendency arranging from strong to weak, ACTIN>CyclinD1>GAPDH. When the goal gene was sampled 10 μ L, the probe density was 15 μ L, 20 μ L was a reasonable dose to reflect a strong-weak tendency. When the gord gene was sampled 20 μ L, the density of goal gene in the hybrid fluid was excessively high regarding ACTIN and CyclinD1, which was unable to reflect the changes of coloration depth. Conclusion: Using the nylon membrane as the carrier to produce gene chip, the chip background is clear. The reasonable amount of goal gene used is 5 μ L, with the optimized probe amount which it matches is 10-20 μ L.