The application effect of isoelectric points(pI) of peptide and protein drugs containing different molecular weights and complex charge heterogeneity was evaluated by capillary isoelectric focusing electrophoresis-whole column imaging detection(cIEF-WCID).The pI of standard polypeptide was mensured repeatedly to verify that cIEF-WCID had a high accuracy and a good repeatability (RSD ＜ 0.50％).Four serum protein isomers had achieved baseline separation.Gan Lai insulin was detected only a single characteristic peaks(pI 5.95 ±0.01) when repeating the test.The proportion differences of the characteristic peak'PI were siguificant,when comparing the recombinant human growth hormone bulk and final,and the final appeared new characteristic peaks.Comparing the imported and domestic Avastin,manufacturer 1 and 3 were found to be consistent with the innovator drug,and the migration behavior of main component in manufacturer 1 was highly consistent with the innovator drug,however,the charge heterogeneity of manufacturer 2 was influenced because of lose K,N-term pyro-Glu.The optimized pI detecting conditions of the recombinant human follicle stimulating hormone were obtained by studying co-solvent concentration,electrolyte and focusing time,namely Co-solvent:2 mol/L urea,ampholytes at pH 2.5-5.0 and pH 3.0-10.0 mixing by 1 ∶ 1,and focus voltage:1 000 V(1 min) to 1 800 V(4 min) and to 2 200 V(1 min).cIEF-WCID was fast and accurate,and it owned excellent resolution and reproducibility,especially,it could trace the focusing process of the analytes.Therefore,it was fit for analysing complex charge heterogeneity of protein drugs.

Objective To make the foundation of further research on the mechanism of AD,we studied the differential proteins in temporal lobes between Alzheimer disease(AD) patients and normal elderly individuals.Methods Human temporal lobes in 12 autopsied individuals were obtained through the Tissue Bank of the Institute of Geriatrics,Chinese PLA General Hospital.They were divided into two groups :normal elderly individuals(n= 6)and Alzheimer' s disease patients(n= 6).We carried out the isobaric tags for relative and absolute quantitation(iTRAQ) coupled with two‐dimensional liquid chromatography‐tandem mass spectrometry(2D LC‐MS/MS) to identify the main differential proteins of the temporal lobe tissue in AD individuals versus the normal elderly individuals.Results Among the 3071 proteins identified, a total of 146 proteins were differentially expressed in AD patients versus normal elderly individuals.We comparatively analyzed the total 146differential proteins using the bioinformatics analysis and identified 7 candidate proteins of interest.ConclusionsThrough the technique of iTRAQ, we can select some differential proteins related with AD, quickly and effectively,which provide the basic data for the effective prevention or targeted therapy of AD.

探寻职业学校班级管理模式,针对150多名来自省内外、经历和家庭背景各异、学习底子薄、基础差、厌学情绪严重、思想复杂、习惯较差的学生,从学生思想和心理工作入手,通过关注细节、交流沟通、加强管理,着力加强班集体建设和班干部培养,促进学生良好习惯和学风的形成。

词汇作为语言的表意单位,是造句、作文的基本.在英语学习及教学过程中,我体会到:大量实质性的困难并非来自语音或语法,而是来自于没有掌握汉英两种语言在词汇方面的差异.由这种差异引起的具体困难尤为明显地表现在英汉翻译方面.

总结自己十几年的班主任工作经验,笔者深深体会到,作为一名特色专业的班主任,要发挥自己在特色专业建设上的职能作用,就要从以下几方面去全身心地投入工作.

目的了解北京市男男性接触者(MSM)关于性传播疾病/艾滋病(STD/AIDS)的知识、态度、信念、行为情况,为在该人群中开展有针对性的干预工作提供依据.方法经过培训的访谈员对符合条件的MSM进行一对一的访谈,并留取唾液样本进行艾滋病病毒Ⅰ型抗体(抗-HIV-1)检测.结果共有481名MSM接受了调查.北京市MSM具有一定的STD/AIDS知识,64.1%的被调查者同时与女性有性接触.近6个月与女性发生阴道交和肛交时每次使用安全套率为27.6%和36.4%,与男性进行肛交或接受肛交时每次使用安全套率为40.1%和38.4%.21%的被调查者自诉患过STD,唾液检测抗-HIV-1阳性率为3.1%.结论北京市MSM人群中存在着STD/AIDS流行,并有向普通人群扩散的潜在危险.