Objective To study the extraction process of Mappianthus iodoies Hand.MaZZ.Polysaccharide and Its Scavenging Effect on Radical.Methods Based on single factor test,the extraction conditions were optimized by orthogonal test.The scavenging capacity of Mappianthus iodoies Hand.MaZZ.Polysaccharide was investigated using.OH and DPPH free radical system.Results The optimal extraction conditions were as follows: extraction temperature of 80℃,liquid ratio of 1∶ 8,extraction duration of 2 h.As concentration of polysaccharide was 1200μg/ml,its scavenging rate on.OH reached 70.65%.As concentration of polysaccharide was 500μg/ml its scavenging rate on DPPH reached 33.54%.Conclusion The optimum extraction technology of Mappianthus iodoies Hand.MaZZ.polysaccharid with water extraction was obtained in this study,and it had strong scavenging effect on hydroxyl radical

OBJECTIVE:To provide scientific cue for the use of Halenia elliptica.METHODS:The crude polysaccharide was extracted from Halenia elliptica with hot water and precipitated by ethanol. The crude polysaccharide has been eliminated protein and fractionated by acidic ethanol. Four fractions HM1, HM2, HM3 and HM4 were got respectively.RESULTS:By using DEAE-Sephadex A-25 gel filtration, HM1 was got. GC analysis indicated that HM1, HM2, HM3, HM4 and HM41 were composed of Rha, Ara, Xyl, Man, Gal and Glc. But their molar ratios were different.CONCLUSION:Polysaccharide HM41 is isolated from Halenia elliptica for the first time.

[Objective] The research aimed extract polysaecharide from Halenia elliptica D.Don.to improve the comprehensive utilization rate of Halenia elliptica D.Don.[Method] Hot water was employed as extracting agent.Three factors,including ratio of liquid-solid,temperature,and time were investigated.[Result] Optimum conditions for extraction was as follows:the ratio of liquid-solid was 10:l,temperature was 80℃,and time for extraction was 1.5h.Under the optimum conditions,the extraction ability of polysaeeharides in Halenia was 2.3%.[Conclusion] The optimized technology could provide reference for the further study of polysaceharides from Halenia elliptica D.Don.

OBJECTIVE:The study was to provided the reference for further development and utilization of Ligularia hodgsonii.METHODS:The crude polysaccharide LW was extracted from Lugularia hodgsonii with hot water, and prectated by ethanol. The crude polysaccharide has been fractionted and purified, so LW21 was got. Its homogeneity was confirmed by chromatography on Sepharose CL-4B, paper chromatography and cellulous actate electrophoresis. The scavenging effets of the polysacehardes on * OH and O2-* were determined by fluorescence method.RESULTS:Antioxidative experiment shows that LW and LW21 were effective in scavenging superoxide radical and hydroxyl radical. The scavenging capacity for superoxide radical and hydroxyl radical of LW21 had higher than that of LW.CONCLUSION:The polysaceharide from Ligularia hodgsonii had stronger antioxidant actioxdant activities, and their antioxidant activities had certain dose-relationship.

In this paper we analyzed the polysaccharide extracted from Dichondra repens Fonst,it contains of no starch,The contents of polysaccharide was 25.17% by using phenol-vitriol method,the content of galactan in polysaccharide was 29.67%.The refined polysaccharide(DF1) was prepared after decolorization and protein being separated.GC analysis indicated that DF1 was composed of Glc.In order to estimate the antioxidation of polysaccharide in D.repens Fonst,two systems of generating superoxide radical,hydroxyl radical respectively in vitro were designed.The result showed that both DF and DF1 were effective in scavenging superoxide radical and hydroxyl radical.The scavenging capacity for superoxide radical and hydroxyl radical of DF1 was higher than that of DF.