独脚金粗多糖(Strigaasiatica (L.) O.Ktze.polysaccharide,SKPc)经DEAE-Sephadex A-25以及SephadexG-100柱层析分离纯化后得到独脚金多糖组分(SKP1*).采用高效液相色谱(HPLC)法检测SKP1*,表明多糖SKP1*极性、相对分子质量是均一的,且其相对分子质量为14852u.采用气相色谱(GC)分析法研究SKP1*的单糖组成为葡萄糖(Glu)、阿拉伯糖(Ara)、半乳糖(Gal)、木糖(Xyl)、甘露糖(Man)、鼠李糖(Rha),相对摩尔比是2.5∶2.0∶0.85∶0.4∶0.5∶1.0;可能主要以β型糖苷键连接.采用化学分析和仪器分析结合进行SKP1*结构分析,表明SKP1*结构是Ara以p-(1→3)糖苷键连接构成主链之一,2,4或2,5位有支链;Gal以β-(1→6)糖苷键连接构成SKP1*的主链之一,且在3位上有分枝.支链由β-(1→4或1→5)Xyl、β-(1→4,1→6,1→2)Glc构成.Rha、Ara、Man、Gal、Glc都有一部分构成糖分子的末端残基.体外清除羟基实验结果表明,SKP1*有较好地清除羟基自由基的活性.

为了获取独脚金多糖(DJPc)的最佳提取工艺条件,采用正交试验设计对水提醇沉法提取DJPc进行优化,并对DJPc进行GC分析和体外清除·OH自由基、DPPH·自由基活性的测定.结果发现得到DJPc的最佳提取工艺条件为料液比1∶18,提取时间2.5h,提取温度80℃,得率为10％;DJPc的单糖组成为Rha、Ara、Xyl、Man、Glc、Gal,其摩尔比为1.0∶0.7∶1.6∶0.8∶1.2∶1.5;在DJPc浓度为2500μg/mL时,得到对·OH自由基和DPPH·自由基的最大清除率,分别为64.9％、50.2％,说明DJPc具有很好的体外抗氧化作用.

Brassica rapa L. is widely used by the Uygur as herbs. Recently more research concentrates on its chemical constituents. The chemical constituents and biological activity of Brassica rapa L. were summarized so as to provide theoretical basis for its further development and utilization.

Objective To study the extraction process of Mappianthus iodoies Hand.MaZZ.Polysaccharide and Its Scavenging Effect on Radical.Methods Based on single factor test,the extraction conditions were optimized by orthogonal test.The scavenging capacity of Mappianthus iodoies Hand.MaZZ.Polysaccharide was investigated using.OH and DPPH free radical system.Results The optimal extraction conditions were as follows: extraction temperature of 80℃,liquid ratio of 1∶ 8,extraction duration of 2 h.As concentration of polysaccharide was 1200μg/ml,its scavenging rate on.OH reached 70.65%.As concentration of polysaccharide was 500μg/ml its scavenging rate on DPPH reached 33.54%.Conclusion The optimum extraction technology of Mappianthus iodoies Hand.MaZZ.polysaccharid with water extraction was obtained in this study,and it had strong scavenging effect on hydroxyl radical

Objective: to isaolate,purify and structurally characterize a water-soluble polysaccharide from the whole herbs of Ligularia hodgsonii.Methods: crude polysaccharide named as LW was extracted from Ligularia hodgsonii.A ploysaccharide fraction named as LW21 was obtained after acidic ethanol fractionation and DEAE-Sephadex A-25 gel filtration.The purity of LW21 was identified by paper chromatography,Sepharose CL-4B chromatography and cellulous acetate electrophoresis,and its structure was analyzed by HIO4 oxidation,Smith degradation,methylation,IR,NMR,GC and GC-MS.Results: LW21 was a homogenous polysaccharide.LW21 was composed of rhamose(Rha),arabinose(Ara),mannose(Man),glucose(Glc) and galactose(Gal) with a molar ratio of 7.4: 11.9: 25.7: 40.0: 14.9.Its average molecular weight was 110 kD.Its main chain was made up of β-(1→2)-linked or β-(1→6)-linked Man,and β-(1→2)-linked orβ-(1→6)-linked Glc residues.The β-(1→2)-linked Man was substituted at 2-O and 4-O;β-(1→6)-linked Man was substituted at 3-O and 6-O;and β-(1→2)-linked Glc was substituted at 6-O;β-(1→6)-linked Gal was substituted at 2-O.The side chain was composed of Rha,Ara and Gal.The non-reduced end was composed of Gal,Ara,Rha and Glc.Conclusion: LW21 is a new polysaccharide isolated from Ligularia hodgsonii for the first time.

OBJECTIVE:To provide scientific cue for the use of Halenia elliptica.METHODS:The crude polysaccharide was extracted from Halenia elliptica with hot water and precipitated by ethanol. The crude polysaccharide has been eliminated protein and fractionated by acidic ethanol. Four fractions HM1, HM2, HM3 and HM4 were got respectively.RESULTS:By using DEAE-Sephadex A-25 gel filtration, HM1 was got. GC analysis indicated that HM1, HM2, HM3, HM4 and HM41 were composed of Rha, Ara, Xyl, Man, Gal and Glc. But their molar ratios were different.CONCLUSION:Polysaccharide HM41 is isolated from Halenia elliptica for the first time.

[Objective] The research aimed extract polysaecharide from Halenia elliptica D.Don.to improve the comprehensive utilization rate of Halenia elliptica D.Don.[Method] Hot water was employed as extracting agent.Three factors,including ratio of liquid-solid,temperature,and time were investigated.[Result] Optimum conditions for extraction was as follows:the ratio of liquid-solid was 10:l,temperature was 80℃,and time for extraction was 1.5h.Under the optimum conditions,the extraction ability of polysaeeharides in Halenia was 2.3%.[Conclusion] The optimized technology could provide reference for the further study of polysaceharides from Halenia elliptica D.Don.