Background:The vaginal microbiota plays a crucial role in women's reproductive health, but its composition and function throughout different female reproductive phases remain poorly understood. This study aimed to investigate the dynamic variations in vaginal microbial communities corresponding to different hormonal states throughout the female reproductive phase. Methods:Vaginal samples were collected from 150 healthy women across five reproductive phases: follicular phase, luteal phase, early pregnancy, lactation, and menopause (30 samples per group). 16S rRNA sequencing was used to analyze the microbial composition. Alpha and beta diversity analyses were performed, and random forest models were constructed to identify key microbial taxa associated with each phase. Functional profiling was conducted using Clusters of Orthologous Groups of Proteins (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Results:Microbial communities show significant variations across the different reproductive phases. Firmicutes dominate in the early reproductive phases but decrease notably during lactation and menopause. Conversely, Bacteroidetes, Actinobacteria, and Proteobacteria increase in later phases. At the genus level, Lactobacillus maintains a higher abundance during reproductive phases but declines during lactation and menopause. Peak microbial diversity occurs during lactation. Functional predictions reveal distinct phase-specific microbial functions. The follicular phase is enriched in pathways associated with nitrotoluene degradation and flavonoid biosynthesis, potentially reflecting estrogen-mediated regulation. Lactation shows enrichment in pathways related to protein digestion and fatty acid metabolism, consistent with increased nutritional demands. Menopause is characterized by enrichment in steroid hormone biosynthesis pathways. In early pregnancy, enrichment of functions related to the adenine nucleotide translocator and phosphate carrier reversible complex suggests functional adaptation of the microbiota to early gestational physiology. Conclusion:Our study reveals dynamic variations in vaginal microbiota across reproductive phases, closely linked to hormonal states, and highlights potential microbial targets for enhancing women's reproductive health.

Background The collagen 3 alpha 1 (COL3A1) rs1800255 polymorphism has been reported to be associated with women pelvic organ prolapse (POP) susceptibility, but the results of these previous studies have been contradictory. The objective of current study is to explore whether COL3A1 rs1800255 polymorphism confers risk to POP. Methods Relevant literatures were searched by searching databases including Pubmed, Embase, Google academic, the Cochrane library, China National Knowledge Infrastructure (CNKI). Search time is from database foundation to March 2021. Results A total of seven literatures were enrolled in the present meta-analysis, including 1642 participants. Overall, no significant association was found by any genetic models. In subgroup analysis based on ethnicity, significant associations were demonstrated in Caucasians by allele contrast (A vs. G: OR = 1.34, 95%CI = 1.03-1.74,), homozygote comparison (AA vs. GG: OR = 3.25, 95%CI = 1.39-7.59), and recessive genetic model (AA vs. GG/GA: OR = 3.22, 95%CI = 1.40-7.42). Conclusions The present meta-analysis suggests that the COL3A1 is a candidate gene for POP susceptibility. Caucasian individuals with A allele and AA genotype have a higher risk of POP. The COL3A1 rs1800255 polymorphism may be risk factor for POP in Caucasian population.

目的 探讨经机器人辅助腹腔镜下阴道骶骨固定术治疗盆腔器官脱垂的效果及安全性.方法 收集2013年1月至2018年12月于解放军总医院第一医学中心妇产科采用经机器人辅助腹腔镜下阴道骶骨固定术治疗盆腔器官脱垂13例患者的临床资料.比较手术前后盆腔器官脱垂治疗效果及安全性,盆腔器官脱垂术后患者采用整体印象改善评分量表(PGI-I)评估主观满意度.结果 13例患者中,平均术中出血量(86.9±98.3) ml(20～300 ml),手术时间(143.5±47.3) min (60～240 min),平均住院时间(10.4±2.1)d(8～16d),平均随访时间(40.8±22.0)个月(6～72个月).脱垂客观治愈率85％,主观治愈率100％,PGI-I评分非常好者9例(9/13),很好者4例(4/13).网片暴露4例(4/13),其中暴露直径小于1 cm且无症状者3例,暴露直径1～2 cm有症状者1例,暴露发生时间均在术后2～12个月,暴露位置均位于阴道缝合范围内.结论 机器人辅助腹腔镜下阴道骶骨固定术治疗盆腔器官脱垂出血量少,住院时间短,术后效果良好,是治疗盆腔器官脱垂可选择的术式之一.