BACKGROUND:The global prevalence of autism spectrum disorder (ASD) is on the rise, and high levels of exposure to toxic heavy metals may be associated with this increase. Urine analysis is a noninvasive method for investigating the accumulation and excretion of heavy metals. The aim of this study was to identify ASD-associated urinary metal markers.METHODS:Overall, 70 children with ASD and 71 children with typical development (TD) were enrolled in this retrospective case-control study. In this metallomics investigation, inductively coupled plasma mass spectrometry was performed to obtain the urine profile of 27 metals.RESULTS:Children with ASD could be distinguished from children with TD based on the urine metal profile, with ASD children showing an increased urine metal Shannon diversity. A metallome-wide association analysis was used to identify seven ASD-related metals in urine, with cobalt, aluminum, selenium, and lithium significantly higher, and manganese, mercury, and titanium significantly lower in the urine of children with ASD than in children with TD. The least absolute shrinkage and selection operator (LASSO) machine learning method was used to rank the seven urine metals in terms of their effect on ASD. On the basis of these seven urine metals, we constructed a LASSO regression model for ASD classification and found an area under the receiver operating characteristic curve of 0.913. We also constructed a clinical prediction model for ASD based on the seven metals that were different in the urine of children with ASD and found that the model would be useful for the clinical prediction of ASD risk.CONCLUSIONS:The study findings suggest that altered urine metal concentrations may be an important risk factor for ASD, and we recommend further exploration of the mechanisms and clinical treatment measures for such alterations.

目的 探讨CARD11与NF-κB及Bcl-2在弥漫性大B细胞淋巴瘤（DLBCL）不同免疫分型中的表达情况和相互作用关系,了解3种分子标记物对DLBCL 的发展过程和临床特征的影响.方法 收集临床资料,选取DLBCL标本70例,反应性增生的淋巴结20例.应用免疫组织化学染色法和组织芯片技术对DLBCL进行免疫分型并检测分型中CARD11、NF-κB和Bcl-2的表达情况.对收集到的数据资料进行整理和统计学分析.结果 70例DLBCL组织标本中,GCB30例,non-GCB40例;Maxvision免疫组化结果显示在DLBCL组织标本70例中,CARD11阳性42例,阳性率60.0%;反应增生性淋巴结中CARD11阳性5例,阳性率25.0%.结论 在DLBCL亚型的分类中CARD11、NF-κB和Bcl-2具有重要的作用,为临床治疗和预防提供了重要参考.

目的:探讨实验室质量控制管理的重点及意义.方法:联系本所微生物实验室的实际情况,讲述实验室质量控制管理的要点.结论:通过从加强微生物检验室质量管理体系、检验人员以及检验质量控制前、中、后阶段的控制,以此来全面做好对微生物检验质量控制工作.

目的 为掌握石岩区域公共场所用品用具的卫生状况,便于更好地监督管理.方法 对2011年对该区域宾馆、旅社、招待所等公共场所公用脸盆、脚盆、毛巾、茶具和浴缸的卫生状况进行了大肠菌群监测.结论 石岩区域公共场所总体不错,但还是受污染的一定程度污染.应加强法制宣传力度,改善公共场所卫生质量.

目的了解石岩区域0～6岁儿童全血中钙、铁、锌、镁、铜、铅含量及相关因素,合理指导本区域儿童膳食。方法随机采集石岩区域2011年1～10月前来体检0～6岁儿童2100例,采集静脉血,使用北京博晖创新光电技术股份公司生产的BH7100型多通道原子吸收光谱仪及配套试测定血中钙、铁、锌、镁、铜及铅的含量及不同年龄组微量元素关系。结果本区域儿童全血中主要以铁、锌、钙缺乏为主,铜、镁基本正常,经统计分析0～3岁组儿童缺铁及缺锌人数高4～6岁组。结论石岩区域儿童存在着不同元素缺乏,提示应加大宣传力度,重视儿童保健,做到早期干预及早治疗。

[Objective]To analyze and evaluate the uncertainty components in measuring nitrates content in water.[Methods]Based on evaluation and denotation of measuring uncertainty(JJF1059-1999),mathematic model of uncertainty was established,and the synthesis uncertainty and expansion uncertainty of nitrate in water by ultraviolet spectrophotometry was obtained.[Results]In determination of nitrates content in water by ultraviolet spectrophotometry,when contents of nitrate were between 0.20~ 8.0 mg/L,synthesis uncertainty was 0.044 mg/L and expansion uncertainty was 0.09 mg/L.[Conclusion]Analysis of measurement uncertainty can control the confidence and accurateness of results,and ensure the quality of measurement.