To investigate the impact of ionizing radiation on the immune system,healthy C57BL/6J mice were irradiated at a dosage of 8 Gy to establish an acute radiation injury model.The survive curve was drawn,and the femurs,the peripheral blood,the thymus and the spleen were obtained at different time points after irradiation.The organ coefficient and the pathology of the spleen and the thymus were examined,the number of hematopoietic stem cells and white blood cells in the bone marrow were determined,and the number of blood cells and levels of GM-CSF and TNF-α in the serum were detected.The results showed that the hematopoietic stem cells and white blood cells in the bone marrow on day 3.5 after irradiation were decreased(P0.05).The number of white blood cells and platelets in peripheral blood are decreased dramatically soon after irradiation(P0.05).Different populations of immune cells in the spleen and the thymus showed obvious differences in sensitivity to radiation with lymphocytesmonocytes,B lymphocytesT cellsNK cells,CD4+ T cellsCD8+ T cells and DCM.The above results show,in addition to the bone marrow,other organs and cells of the immune system are very sensitive to radiation also.So difference in the sensitivity to radiation among various subsets of immune cells may be the main cause of radiation-induced immune disorders and related pathological injuries.

Objective To investigate and analyze the RhD(-),RhE(-) and RhC(-) of Rh blood group system and to provide the experiment basis for preventing hemolytic transfusion reaction(HTR) and newborn hemolytic disease(NHD).Methods 11 035 cases of blood samples were examined for ABO,RhD,RhE and RhC blood groups by using micro-column gel method.Results Among all of the detected samples,0.21%(23/11 035) were with RhD(-),47.8%(5 275/11 035)with RhE(-) and 7.6%(839/11 035) with RhE(-).Conclusion To take Rh blood group system,including RhD,RhE and RhC,as routine blood group tests might have important clinical significance in the prevention of HTR and NHD,as well as in the improvement of the safety of a blood transfusion treatment.

本文建立氚水染毒小鼠模型，采用甲基化DNA免疫沉淀-芯片(MeDIP-chip)杂交技术分析小鼠肝组织基因启动子CpG甲基化情况，并检测了肺、肝、肾脏组织DNMT1的表达水平。结果显示，不同浓度氚染毒10 d的小鼠肝脏组织基因组出现了新的甲基化位点，而且高浓度和低浓度染毒组的共同发生甲基化的基因有19个，17个发生在启动子的CpG。随着氚水初始注入量增加，累积剂量增大，肺、肝、肾组织DNMTl表达量相应降低。氚水初始注入量为5.55×105 Bq/g时，随注入后时间延长，累积剂量增大，DNMTl表达量下降后再恢复。实验结果提示，甲基化改变的差异基因可能在氚水内照射损伤时起调控作用，不同组织中DNMT1的检测可用于辐射损伤的早期检测或损伤恢复的评估。