The seeds and the top of stalk of terrestrial orchid as explants were cultured on medium.The tissue culture of terrestrial orchid were studied.The results were as follows: The best culture medium for seed germinate of terrestrial orchid was 1/2 MS+NAA 0.3 mg/L+IBA 3.0 mg/L +active carbon(AC) 2 g/L+1 g/L(culture liquid of the orchifd from Thailand);The best medium in inducing protocorm was 1/2 MS+NAA 0.4 mg/L+6-BA 3.0 mg/L +(AC) 2 g/L+fresh coconut juice(CM) 250 mL/L+1% glucose+micro-vitamin C,The best medium in adventitious buds induced was 1/2 MS+NAA 0.4 mg/L+6-BA 4.0 mg/L +AC 2 g/L;The best medium in rooting of adventitious buds was 1/2 MS+NAA 0.2 mg/L+AC 2 g/L.