Objective:To establish an HPLC-ELSD method for the determination of peimine and peiminine in Hechan tablets.Methods:Chromatography was performed on a HiQsil C_(18) column(250 mm×4.6 mm,5μm)at 25 ℃.The mobile phase composition was acetonitrile-methanol-diethylamine(60:40:0.05)with a flow rate of 0.8 mL·min~(-1).An evaporate light-scattering detector(ELSD)was used as detector with gas flow rate of 2.0 L· min~(-1) and drift tube temperature of 80℃.Results:The calibration curves of peimine and peiminine were linear in the ranges of 0.29-2.80μg and 0.34-3.40μg,and their regressive equations were Y=1.557X+1.666(r= 0.9996)and Y=1.700X+1.571(r=0.9999)respectively.The average recoveries(n=5)of peimine and peimi- nine were 99.2%(RSD=2.7%),and 99.4%(RSD=3.1%),respectively.Conclusion:The method is reliable, simple,and accurate.It can be used to control this preparation.

OBJECTIVE Summarize the reserach progress on Genus paris.METHODS Sum up the progress in the research of chemical constituents and biological activities in Genus Paris.RESULTS and CONCLUSION The chemical constituents have new structures and good activities.Genus paris posses great value.