目的:研究中药添加剂降低烟气悬凝物诱导16HBE细胞TNF-α、IL-8及MUC5AC mRNA高表达的作用.方法:CCK-8法检测细胞活力,RT-PCR法检测TNF-α、IL-8及MUC5AC mRNA表达水平.结果:CSC染毒后细胞活力下降,TNF-α、IL-8及MUC5AC mRNA表达水平升高.中药添加剂组细胞活力高于普通烟组,mRNA表达水平有所下降.结论:中药添加剂具有抑制CSC诱导16HBE细胞活力下降及TNF-α、IL-8及MUC5AC mRNA高表达的作用.

Objective: To investigate the effect of Chinese herbal additives on 16HBE cell injuries induced by smoke condensate.Methods: Methods: Human bronchial epithelial cell line 16HBE cells were used to establish smoke condensate exposure model to detect the cell viability,oxidative stress and secretion of TNF-α,IL-8 after exposure to toxin.Results: After exposure to smoke condensate,the cell viability decreased,oxidative stress levels were elevated,the secretion of TNF-α,IL-8 increased.In Chinese herbal additive group,after exposure to smoke condensate,cell viability was higher than that of normal group,LDH release and oxidative stress levels decreased,the secretion of TNF-α and IL-8 reduced.Conclusion: 16HBE cells exposed to smoke condensate can decrease the cell viability and increase the oxidative stress levels and cytokine secretion,Chinese herbal additives can alleviate the above condition.