Cells of Dictyostelium discoideum are trigged to undergo development when they are depleted their food source.Cell cytoskeleton proteins play an important role for completing the development of Dic- tyostelium discoideurn.To study the change of cytoskeleton proteins during Dictyostelium discoideum de- velopment,we used the wild-type cells KAx-3 and mutant cells AK127;cytoskeleton ghost have been pre- pared by biochemical extraction with TrotonX-100,cytoskeleton proteins were separated using SDS-PAGE and scan by BIO-PROFILE of VILBER LOURMAT.The datum showed that the 103.4 kD、49.6 kD、44.2 kD、30.8 kD、19. 8kD and 13.5 kD were shared by KAx-3 and AK127 cells;in that 44.2 kD and 30.8 kD were the most stable.They may take some support for cell development with other cytoskeleton proteins. The most different existed in some proteins,for example 87.2 kD,68.2 kD and 40.7 kD,those proteins can not be expressed in the mutant cells at the same developmental stage of wild type cells.This result indicates those proteins are required for Dictyostelium cells development.It is noteworthy that mutant cell showed the characteristic 21.1 kD proteins after 12 h development.These obvious difference related to the process of development,in other hand,also related to the mutant cell lacking gp150 protein.

Studying the morphogenesis of Dictyostelium discoideum development, we found that there were distinct differences between the wild cells KAX-3 and the mutant cells AK127( LagC gene was knockout). The mutant cells AK127 was blocked at loose aggregation stage for lacking gp150 during developmental stages. However, the wild cells KAX-3 completed the whole developmental stages successfully. Three isoenzymes were obtained from developing cells of Dictyostelium discoideum which included a wild type cells (KAX-3) and a mutant type cells Compared these isoenzymes by gel electrophoresis, we found that two different esterases appreared in AK127 while only one in KAX-3. Furthermore, the content of malate dehydro-genase (MDH) is more in AK127 than in KAX-3 at different developing stage. At 14h during development, the content of glutamate dehydrogenase(GDH) in AK127 is higher than in KAX-3; but at 16h GDH in AK127 is lower than in KAX-3. From the results, we learned that the pattern of some isoenzymes were changed between wild cells and mutant cells during development stage, indicating some changes in the expression of some genes. So it seemed that gp150 was an important factor in development.

本文综述了盘基网柄菌(Dictyostelium dis-coideum)发育过程中细胞类型的诱导和分化,细胞外cAMP及其四种位于细胞表面的受体及PKA(蛋白激酶A)、GSK-3(糖原合成酶激酶)和STATa等在网柄菌发育过程中的作用.