AIM:To compare three different solvent extraction procedures affecting the extraction rate of polysaccharide from Schisandra chinensis (Turcz.). METHODS: We used single factor experiments, optimized the extraction process of polysaccharide from Schisandra chinensis (Turcz.) by alkali solution and ethanol-alkali solution, respectively, and contrasted the two methods with the water extraction method that had been reported, determined the extraction rate of polysaccharide by optimum condition of three kinds of solvent. RESULTS: These results showed that ethanol-alkali extraction procedure had the highest extraction rate, 1.12 times more than water extraction procedure and 1.06 times more than alkali extraction procedure. CONCLUSION: Ethanol-alkali extraction procedure is a high effective and stable for polysaccharide from Schisandra chinensis (Turcz.).

目的:比较3种不同溶剂提取的五味子多糖的抗氧化和抑菌作用。方法:通过多糖对O2-.和.OH的抑制作用来确定多糖的抗氧化效果,通过抑菌圈实验来确定多糖的抑菌效果。结果:醇碱法所提取的五味子多糖的抗氧化效果优于水提法和碱水法所得多糖,而抑菌效果则没有明显差异。结论:醇碱法是一种所得多糖抗氧化效果和抑菌效果较好的五味子多糖提取方法。